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COMPARATIVE STUDY OF BACTERIA FOR
BIOSURFACTANT PRODUCTION BY USING
DIFFERENT CARBON SOURCES
BY:
-ABHISHEK M SIRSIKAR
-SAMEER S. HADAWALE
-ANSHULKUMAR GUPTA
VIT UNIVERSITY, VELLORE
Synopsis:
Introduction
Importance of biosurfactant in bacteria
Pathways for biosurfactant production
Objective
Experimental Details
Applications
Introduction
 Surfactants both chemically and biologically are
amphiphilic compounds.
 Biosurfactants possess different chemical
structures--lipopeptides, glycolipids, neutral lipids
and fatty acids.
 They are nontoxic biomolecules that are
biodegradable.
Importance of biosurfactant in bacteria
The low water solubility of these
hydrophobic compounds limits their
availability to microorganisms.
 They also have vital importance to the
microbes in adhesion, emulsification,
bioavailability, desorption and defense
strategy.
It plays an essential role in the swarming
motility of microorganisms and participate
in cellular & physiological processes of
signalling and differentiation as well as in
the biofilm formation.
 It have antibacterial and antimicrobial
property.
Pathways for biosurfactant production:
Examples of biosurfactant
Objective
Our moto is to identify the efficient
biosurfactant producing bacteria under
controlled supply of different Carbon sources.
And see the ability of bacteria how much
yield it produced by giving different carbon
sources such as dextrose, yeast,beef and
peptone.
Screening
Isolation and culturing
Inoculum Preparation
Inoculation and Incubation
Extraction
Qualitative Analysis
Experimental Details
Tests
Oil Displacement
Emulsification Index
Hemolytic Activity
Biosurfactant Yield
Agriculture (pest control, biofertilizers)
Washing, hygienic, cosmetic means
Inhibition of corrosion
Pharmaceutical industry
Food industry
Bioremediation of soils and water
Applications:
The directions of R&D
Investigation of synthesis of biosurfactants, their
properties and application in biomedicine, agriculture,
food, pharmaceutical and cosmetic industry.
Development of methods of biotechnological
remediation of water and soil
Biosynthesis and studying of microbial enzymes and
their compositions
Creation and investigation of new polymeric materials
Monitoring of petroleum-contaminated water and soil
Thank You very much!

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Biosurfactant

  • 1. COMPARATIVE STUDY OF BACTERIA FOR BIOSURFACTANT PRODUCTION BY USING DIFFERENT CARBON SOURCES BY: -ABHISHEK M SIRSIKAR -SAMEER S. HADAWALE -ANSHULKUMAR GUPTA VIT UNIVERSITY, VELLORE
  • 2. Synopsis: Introduction Importance of biosurfactant in bacteria Pathways for biosurfactant production Objective Experimental Details Applications
  • 3. Introduction Surfactants both chemically and biologically are amphiphilic compounds. Biosurfactants possess different chemical structures--lipopeptides, glycolipids, neutral lipids and fatty acids. They are nontoxic biomolecules that are biodegradable.
  • 4. Importance of biosurfactant in bacteria The low water solubility of these hydrophobic compounds limits their availability to microorganisms. They also have vital importance to the microbes in adhesion, emulsification, bioavailability, desorption and defense strategy. It plays an essential role in the swarming motility of microorganisms and participate in cellular & physiological processes of signalling and differentiation as well as in the biofilm formation. It have antibacterial and antimicrobial property.
  • 7. Objective Our moto is to identify the efficient biosurfactant producing bacteria under controlled supply of different Carbon sources. And see the ability of bacteria how much yield it produced by giving different carbon sources such as dextrose, yeast,beef and peptone.
  • 8. Screening Isolation and culturing Inoculum Preparation Inoculation and Incubation Extraction Qualitative Analysis Experimental Details
  • 11. Agriculture (pest control, biofertilizers) Washing, hygienic, cosmetic means Inhibition of corrosion Pharmaceutical industry Food industry Bioremediation of soils and water Applications:
  • 12. The directions of R&D Investigation of synthesis of biosurfactants, their properties and application in biomedicine, agriculture, food, pharmaceutical and cosmetic industry. Development of methods of biotechnological remediation of water and soil Biosynthesis and studying of microbial enzymes and their compositions Creation and investigation of new polymeric materials Monitoring of petroleum-contaminated water and soil
  • 13. Thank You very much!