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The QuantStudio 12K Flex Real-Time PCR System is an all-in-one instrument designed for high throughput real-time PCR. It has five interchangeable blocks that allow it to analyze from 1 to over 12,000 samples in a single run. The OpenArray format can analyze up to four plates with 3,072 reactions each, generating over 12,000 data points per run. The system offers flexibility in sample volume and format, easy block changes, and software for integrated analysis and sample tracking from loading to results.

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The all-in-one qPCR instrument QuantStudio 12K Flex Real-Time PCR System
Expand the boundaries of your research Life Technologies is taking qPCR to the next level. Designed for maximum throughput, flexibility, and scalability, the QuantStudio 12K Flex Real-Time PCR System sets the new standard for what a real-time PCR instrument should be. Combining flexible throughput capabilities with a streamlined workflow, intuitive software, and a state-of-the-art industrial design, this is the one instrument that can take you from targeted discovery through confirmation and screening, and everything in between. 2
Maximum throughput, minimal resources The QuantStudio 12K Flex system can simultaneously run up to four 3,072-reaction QuantStudio 12K Flex OpenArray速 Plates in about 4hours. A streamlined workflow and automated plate handling minimize start-up and hands-on times. Outstanding flexibility TaqMan速 Assays are available for all 5 thermal cycling blocks: OpenArray速 , TaqMan速 Array Card, 384-well, and standard or Fast 96-well blocks. Block changes typically take less than 1 minute and require no tools, providing the ultimate in format flexibility. Achieve consistent, high-quality results across different plating formats as your research progresses. Scales to your research With five interchangable thermal cycling blocks, you can analyze from 1 to over 12,000 data points per run in the assay format thats right for your projecttoday and in the future. 3
Maximum throughput, minimal resources Ultimate combination of speed, throughput, and data analysis capabilities Maximum speed and throughput. The OpenArray速 format can accelerate genomic confirmation and screening programs by generating up to 110,000 data points or more per 8hour day, completing your project in days rather than weeks. Simple workflow. Load your samples onto the plate (Figure 1) using the QuantStudio 12K Flex AccuFill System, then run up to four plates to generate over 12,000 data points per run. Integrated software tracking features help you easily map and track your samples, giving you the ultimate confidence in your results. Reduce start-up and hands-on time. Start a 12,000 data point experiment in typically less than 20 minutes using automated sample loading with the QuantStudio 12K Flex AccuFill System (Figure 2). Integrated analysis and quality systems. Comprehensive software analysis tools for gene expression, genotyping, and digital PCR are available at your fingertips. Laboratory Information Management Systems (LIMS) and features that assist with 21CFR Part 11 compliance are enabled for high-throughput, validated environments. Economical processing. Save precious samples and reduce reagent costs through cost-effective usage of nanoliter volumes. The QuantStudio 12K Flex Real-Time PCR System sets a new standard for automated analysis for researchers conducting large genotyping or gene expression studies. The streamlined OpenArray速 workflow helps save time and resources compared to running experiments in multiple 384-well plates. When equipped with the OpenArray速 block and the QuantStudio 12K Flex OpenArray速 AccuFill System, the QuantStudio 12K Flex system can produce up to 110,000 data points or more in an 8-hour day with minimal training, as little as 20 minutes of hands-on time per run, and no third-party robotics. Figure 1. QuantStudio 12K Flex OpenArray速 Plates are supplied in an alloy case for easy handling. After loading samples, place a lid on the array and fill with immer- sion fluid. The QuantStudio 12K Flex system can run up to four plates at once. 4
OpenArray速 technology OpenArray速 technology is a broadly applicable nanoliter fluidics platform for low-volume, solution-phase reactions. Benefit from the parallelism of microarrays and the data quality of solution-phase reactions such as PCR with the QuantStudio 12K Flex system. QuantStudio 12K Flex OpenArray速 plates are microscope slidesized and are arranged in 48subarrays of 64 through-holes, with a total of 3,072through-holes for individual 33nL reactions (Figure 3). Plates are coated with hydrophilic and hydrophobic compounds to retain reagents in through-holes via surface tension. Subarray Through-holes retain 33 nL reaction mixtures via surface tension Hydrophobic Hydrophilic Figure 3. OpenArray速 plate technology. Figure 2. The QuantStudio 12K Flex AccuFill System simplifies sample loading into QuantStudio 12K Flex OpenArray速 Plates. 5
Flexibility No compromises necessaryfull compatibility with diverse applications, plus single-tube to 12,000 reaction/run throughput Five interchangeable blocks. Choose and load the thermal-cycling block you need for the experiment at hand. Additional blocks are available to scale your throughput capability to up to 12,000 data points in a single run. Provides a seamless switch from qPCR to digital PCR. Increase the precision and sensitivity of your experiments by switching from real- time PCR to digital PCR mode using QuantStudio digital PCR kits and DigitalSuite Software with the QuantStudio OpenArray速 block. From late Ct values to undetermined copy numbers, digital PCR provides a method to rescue ambiguous qPCR results, increasing the success rate of your experiments. Multiplexing flexibility. With up to six independent excitation and emission filter channels, the QuantStudio 12K Flex system flexibly accommodates your real-time chemistry needs and provides ample multiplexing capability. Dual-color detection is enabled for QuantStudio 12K Flex OpenArray速 plates. Impressive throughput without complicated robotics. With 384-well TaqMan速 Array Cards, theres no need for liquid-handling robotics or complex pipetting to load; simply add your sample and master mix and run on the QuantStudio 12K Flex system. Its often the unexpected results in the lab that lead to the important breakthroughs. With this one instrument, your lab can be ready for many types of experiments and users, from low- to high-throughput sample processing and virtually any PCR application that requires fluorescence detection. 6
Figure 4. Real-time PCR reproducibility and 9-log dynamic range. Amplification plot shows results from real-time PCR of 18S DNA in 10-fold dilutions starting at 25,000 copies using the 384-well block. The data show highly reproducible results for input target quantities cover- ing nine orders of magnitude (9 logs). These data illustrate the broad dynamic range of the system. Cycle 24 4020161210 388 364 6 342 32302826221814 Rn 0.1 1 10 0.01 Enhanced OptiFlex速 System The QuantStudio 12K Flex Real-Time PCR System uses a white light LED optics system enabling powerful, extremely accurate and sensitive data collection (Figure 4). Additionally, the white light LED provides a broad spectrum of light for maximum resolution of 12,000 data points. The Enhanced OptiFlex速 System can also capture QuantStudio 12K Flex OpenArray速 plate identities from their barcodes. You can instantly load the appropriate experiment files for one-touch runs to help ensure that the plates were loaded correctly. 7
Take on the experiments youve always wanted to do, the QuantStudio 12K Flex system has the capabilities youll need to follow your research leads with ease. Start your project with lower-throughput feasibility experiments and then scale up to get statistically significant answers for publishing groundbreaking results. Quick, simple block changes. Thermal blocks are easy to change for all users in the laband no tools or service calls are needed. In just minutes, you can get the QuantStudio 12K Flex system ready for experiments requiring just a few data points per runor thousands. Ample data storage. Know that your data are always secure. The QuantStudio 12K Flex system can store data from over 100 runs without an external database, so your productivity wont be limited by data storage concerns. Fully compatible with TaqMan速 Assay formats and chemistry. As you scale your experiments and increase throughput, be assured that TaqMan速 Assay sensitivity, specificity, and wide dynamic range are optimized across all QuantStudio 12K Flex system block formats. Automation capability. With the QuantStudio 12K Flex Twister速 II Automation Robot (Figure 5), you can automate your real-time PCR runs to accommodate sample throughput increases. Scalability A single instrument for your real-time PCR needs today with the capabilities youll need tomorrow Figure 5. The QuantStudio 12K Flex System can be fully integrated with the Twister速 II Robot for real-time PCR in automated environments. 8
Single-tube TaqMan速 Assays 384-well TaqMan速 Array Cards TaqMan速 Assays in 96- and 384-well plates QuantStudio 12K Flex OpenArray速 plates 9
QuantStudio 12K Flex Software User-friendly Feature-rich touch screen interface makes it easy to get started and keep going (Figure 6). Intuitive interface. Easily identifiable icons on the touch screen guide you through the workflow to set up runs and analyze experiments. The graphical interface makes it easy to edit thermal cycling conditions for your 96-well, 384-well, and TaqMan速 Array Card blocks, and enables one-touch runs for the OpenArray速 block. Sample tracking from start through analysis. The OpenArray速 SampleTracker helps you take your samples from 96-well plate format, to visually planning your OpenArray速 plate configuration, to the AccuFill Software for loading your OpenArray速 plates, and finally integrates with QuantStudio 12K Flex Software for one-touch runs. Real-time monitoring. Monitor up to 15 instruments simultaneously and view amplification plots from subarrays. A data analysis suite at your fingertips Innovative software applications to analyze your data. Enhanced gene expression analysis. ExpressionSuite Software v1.0 can analyze data from small or large projects, even 100+ runs. Quickly check data quality and perform sophisticated statistical analysis to understand expression patterns and relationships between samples. Real-time control for genotyping cluster analysis. You can optimize your genotyping run times using a real-time PCR progress monitor to determine the ideal cycle for cluster analysis. Straightforward transition to digital PCR. With DigitalSuite Software v1.0 you can increase the precision and sensitivity of your qPCR experiments by switching to digital PCR mode (Figure 7). Digital PCR provides new capabilities to help you get more from your experiments. 10
Figure 6. Easy analysis of OpenArray速 runs: toggle between targets, samples, and subarray. High-throughput capable Easily keep track of your samples and your results High-throughput experiment setup. Create multiple experiment files simultaneously with the push of a single button and match them with your unique sample files. You can import your plate setup files from other Applied Biosystems速 real-time systems to facilitate experiment setup. Easy, automated data export. Set up experiment templates with export preferences that direct the system software to auto-export your results to a file or email them to you. LIMS compatibility. Open application program interfaces (API) allow integration with third-party systems such as LIMS (laboratory integration management systems) or custom automated platforms. The optional 21 CFR Part 11 compliance module assists with security, auditing, and e-signature records for data traceability. Figure 7. Analyze your digital PCR results using DigitalSuite Software for absolute quantitation with greater sensitivity and precision of rare- target detection. 1/400 1/200 1/100 1/10 0.039copies/rxn 0.062copies/rxn 0.122copies/rxn 1.210copies/rxn 0.059copies/rxn 0.116copies/rxn 0.286copies/rxn 3.060copies/rxn 0.097copies/rxn 0.165copies/rxn 0.362copies/rxn 3.508copies/rxn 0.1 1 10 100 Concentration (copies/nL) 1 10 100 Concentration (copies/nL) 1 10 100 1000 Concentration (copies/nL) 11
Applications & technologies A wide range of TaqMan速 Assays offer superior performance for both routine and challenging applications Gene expression analysis. TaqMan速 Gene Expression Assays are the most comprehensive set of quantitative gene expression assays available, providing more than 1.3 million primer/probe sets for 23 species, in four sizes, including your choice of FAM or VIC速 dye labels. Custom assays are available for studying the expression of any gene or splice variant in any organism. SNP genotyping. The precision of TaqMan速 probebased chemistry makes SNP genotyping studies easy. Choose from over 4.5 million predesigned human and mouse TaqMan速 SNP Genotyping Assays and 2,700 TaqMan速 DME Genotyping Assays, or Custom TaqMan速 SNP Genotyping Assays, in various sizes. Copy number analysis. TaqMan速 Copy Number Assays are a collection of over 1.6 million predesigned assays with genome-wide coverage for human, over 180,000 assays targeting exons for mouse, and Custom Plus and standard Custom TaqMan速 Copy Number Assays when a predesigned assay is not readily available. Digital PCR technology. Ideal for sensitive detection of rare targets such as in cancer mutations, viruses, and GMO detection. In digital PCR, reactions are split into hundreds to thousands of mini-reactions to provide a digital readout of real-time PCR results. castPCR technology. TaqMan速 Mutation Detection Assays are designed to detect and measure DNA mutations with extremely high specificity against a background of wild- type genomic DNA (gDNA). These assays are powered by competitive allele-specific TaqMan速 PCR technology, known as castPCR technology, for detection of mutations present at less than 0.1% in gDNA. MicroRNA and other noncoding RNA analysis. Innovative TaqMan速 Assays and tools are available for long noncoding RNA quantification, miRNA profiling, pri-miRNA quantification, small RNA quantification, and targeted miRNA quantification. High resolution melt (HRM) analysis. HRM analysis is a rapid, economical method for mutation scanning and methylation analysis in genomic DNA. 12
Pharmacogenomics panels. For researchers involved in pharmacogenomics, our fixed-content panel of selected TaqMan速 DME assays is ideal for high-throughput analysis of the effects of genes on human responses to drugs. This panel contains all known high-value DME pharmacogenetics markers, including PharmaADME core markers. Barcoding panels. For high-throughput tracking and confirmation of sample identity and gender within a large biorepository setting, our fixed-content OpenArray速 panels contain a defined set of selected autosomal and Y chromosome SNP markers common to commercial arrays. Gene expression pathway panels. For researchers performing disease studies, these fixed-content gene expression OpenArray速 Pathway Panels are for high- throughput analysis of commonly studied, disease-related gene families. These include human cancer, kinome, inflammation, signal transduction, and stem cell panels, and mouse inflammation panels. Innovative assay panels to take your research to the next level Life Technologies research and development scientists continue to develop new collections of TaqMan速 Assays to use with Applied Biosystems速 real-time PCR technology. Most recently weve introduced the following novel products: 13
Description Specification Block configurations and volumes 96-well (10100 袖L reactions), Fast 96-well (1030 袖L reactions) 384-well (520 袖L reactions) TaqMan速 Array Cards (~1 袖L reactions) OpenArray速 Plates (33 nL reactions) Block change design Block change from front in less than 1 min; no tools or service call required Excitation source Enhanced OptiFlex速 system, white LED Instrument control Instrument touch screen, networked computer, or attached computer Detection channels Decoupled: 6 emission, 6 excitation (96-well/Fast, 384-well, TaqMan速 blocks) Coupled: 4 emission, 4 excitation (OpenArray速 blocks) 21 CFR p11 enablement Optional software module Dimensions (W x D x H) 50.5 cm x 67.2 cm x 73.8 cm Weight 69kg(152lb) Power 100240 V Remote monitoring Available to monitor up to 15 networked instruments simultaneously Data export format User configurable: *.xls, *xlsx, *.txt 96-well, 96-well Fast, 384-well, TaqMan速 Array Card blocks OpenArray速 block Detection channels Decoupled: 6 emission, 6 excitation Coupled: 4 emission, 4 excitation Well-to-well variability 賊 0.25属C 賊 0.75属C Max block ramp rate 3.0属C/sec (384-well) 3.0属C/sec Run time 30 min expected (Fast 96-well block) 35 min (384-well block, using Fast master mix) 2 hr (gene expression) 4 hr (genotyping) Demonstrated sensitivity To 1 copy To 1 copy Dynamic range To 9 logs To 7 logs Resolution As low as 1.5-fold change for singleplex reaction As low as 2-fold change for singleplex reaction Hardware specifications QuantStudio 12K Flex Real-Time PCR System 14
Software Description Blocks supported Embedded in QuantStudio 12K Flex Software v1.0 SampleTracker v1.0 Software Facilitate sample tracking from 96-well to 384-well plates Illustrates mapping to OpenArray速 plates QuantStudio 12K Flex OpenArray速 block HRM Software Module for QuantStudio 12K Flex system High resolution melt analysis 96-well, 384-well blocks 21 CFR Part 11 Software Module for QuantStudio 12K Flex system Quality control system compliance features All blocks Downstream applications accessible from QuantStudio 12K Flex software home screen DigitalSuite Software Digital PCR analysis that enables duplex analysis, one-click launch, amplification curves, and heat maps All QuantStudio 12K Flex system blocks ExpressionSuite Software Gene expression analysis software supporting up to 100 files with enhanced performance time. The software features relative quantification with p-value, correlation plots, box plots, heat maps, and volcano plots All QuantStudio 12K Flex system blocks TaqMan速 Genotyper Software Improved version of existing software product that includes single-plate views All QuantStudio 12K Flex system blocks Software specifications Download from www.appliedbiosystems.com 15
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Kevin Munelly, Life Technologies The highly flexible QuantStudio 12K Flex system allows you to perform the experiments that were difficult in the past. 17
Applied Biosystems速 service plans offer: Highly valued field services Protect your technology investment with a wide range of service plans that fit your laboratory requirements and budget needs. Highly trained, certified service engineers use the latest technology to quickly diagnose and fix your instruments. Realize up to 45% savings on instrument care through purchase of a service plan. Award-winning remote services Remotely and proactively monitor, alert, diagnose, and correct your instrument performance for maximum uptime. Avoid unplanned interruptions in lab operations with automatic alerts sent to your field service engineer (FSE). Remotely track critical system parameters with real-time reporting. Compliance and validation services Broad range of audit-quality services, including installation qualification (IQ), operation qualification/instrument performance verification (OQ/IPV), pure dye calibration, on-site temperature verification, computer system validation, and risk assessment. Comprehensive packages with rigorously executed protocol documentation including all the data collected. A partnership with you that utilizes the latest technology to move quickly into testing and production using audit-quality expert support to minimize the frustration and burden of developing and maintaining compliance and change control. Applied Biosystems速 service plans Applied Biosystems速 service & support AB Complete AB Assurance AB Maintenance Repair response time (business days) Guaranteed next day: on-site repairs Guaranteed 2-day: on-site repairs Target 2-day: remedial repairs Remote instrument monitoring and diagnostics + + Priority phone and email access to instrument support + + Priority phone and email access to application technical support + + + Instrument operating software upgrades + Instrument qualification services + Planned maintenance + + + Parts, labor, and travel Included Included Discount with Plus plan 18
Instrument systems (with desktop computer) Cat. No. QuantStudio 12K Flex Real-Time PCR System with OpenArray速 Block (includes AccuFill system) 4471090 QuantStudio 12K Flex Real-Time PCR System with OpenArray速 Block (without AccuFill system) 4472380 QuantStudio 12K Flex Real-Time PCR System with TaqMan速 Array Card Block 4471089 QuantStudio 12K Flex Real-Time PCR System with 384-Well Block 4471134 QuantStudio 12K Flex Real-Time PCR System with 96-Well Fast Block 4471088 QuantStudio 12K Flex Real-Time PCR System with 96-Well Block 4471087 Additional thermal cycling blocks and accessories 96-Well Block Upgrade Kit 4453543 96-Well Fast Block Upgrade Kit 4453544 384-Well Block Upgrade Kit 4453545 TaqMan速 Array Card Block Upgrade Kit 4453546 OpenArray速 Block Upgrade Kit with AccuFill System 4471067 QuantStudio 12K Flex AccuFill Upgrade Kit (For existing AccuFill system users) 4471022 QuantStudio 12K Flex Twister 速 II Automation Robot 4471066 Applied Biosystems速 Service Plans Applied Biosystems速 Complete Service Plan Contact us Applied Biosystems速 Assurance Service Plan Contact us Applied Biosystems速 Maintenance Service Plan Contact us Ordering information 19
lifetechnologies.com For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 息 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan速 is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Twister is a registered trademark of Caliper Life Sciences Inc. Printed in the USA. CO01523 1211 For more information about the QuantStudio 12K Flex Real-Time PCR System or any of our real-time PCR products, contact your Life Technologies representative or visit www.appliedbiosystems.com/quantstudio

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Biosystem prosedur

  • 1. The all-in-one qPCR instrument QuantStudio 12K Flex Real-Time PCR System
  • 2. Expand the boundaries of your research Life Technologies is taking qPCR to the next level. Designed for maximum throughput, flexibility, and scalability, the QuantStudio 12K Flex Real-Time PCR System sets the new standard for what a real-time PCR instrument should be. Combining flexible throughput capabilities with a streamlined workflow, intuitive software, and a state-of-the-art industrial design, this is the one instrument that can take you from targeted discovery through confirmation and screening, and everything in between. 2
  • 3. Maximum throughput, minimal resources The QuantStudio 12K Flex system can simultaneously run up to four 3,072-reaction QuantStudio 12K Flex OpenArray速 Plates in about 4hours. A streamlined workflow and automated plate handling minimize start-up and hands-on times. Outstanding flexibility TaqMan速 Assays are available for all 5 thermal cycling blocks: OpenArray速 , TaqMan速 Array Card, 384-well, and standard or Fast 96-well blocks. Block changes typically take less than 1 minute and require no tools, providing the ultimate in format flexibility. Achieve consistent, high-quality results across different plating formats as your research progresses. Scales to your research With five interchangable thermal cycling blocks, you can analyze from 1 to over 12,000 data points per run in the assay format thats right for your projecttoday and in the future. 3
  • 4. Maximum throughput, minimal resources Ultimate combination of speed, throughput, and data analysis capabilities Maximum speed and throughput. The OpenArray速 format can accelerate genomic confirmation and screening programs by generating up to 110,000 data points or more per 8hour day, completing your project in days rather than weeks. Simple workflow. Load your samples onto the plate (Figure 1) using the QuantStudio 12K Flex AccuFill System, then run up to four plates to generate over 12,000 data points per run. Integrated software tracking features help you easily map and track your samples, giving you the ultimate confidence in your results. Reduce start-up and hands-on time. Start a 12,000 data point experiment in typically less than 20 minutes using automated sample loading with the QuantStudio 12K Flex AccuFill System (Figure 2). Integrated analysis and quality systems. Comprehensive software analysis tools for gene expression, genotyping, and digital PCR are available at your fingertips. Laboratory Information Management Systems (LIMS) and features that assist with 21CFR Part 11 compliance are enabled for high-throughput, validated environments. Economical processing. Save precious samples and reduce reagent costs through cost-effective usage of nanoliter volumes. The QuantStudio 12K Flex Real-Time PCR System sets a new standard for automated analysis for researchers conducting large genotyping or gene expression studies. The streamlined OpenArray速 workflow helps save time and resources compared to running experiments in multiple 384-well plates. When equipped with the OpenArray速 block and the QuantStudio 12K Flex OpenArray速 AccuFill System, the QuantStudio 12K Flex system can produce up to 110,000 data points or more in an 8-hour day with minimal training, as little as 20 minutes of hands-on time per run, and no third-party robotics. Figure 1. QuantStudio 12K Flex OpenArray速 Plates are supplied in an alloy case for easy handling. After loading samples, place a lid on the array and fill with immer- sion fluid. The QuantStudio 12K Flex system can run up to four plates at once. 4
  • 5. OpenArray速 technology OpenArray速 technology is a broadly applicable nanoliter fluidics platform for low-volume, solution-phase reactions. Benefit from the parallelism of microarrays and the data quality of solution-phase reactions such as PCR with the QuantStudio 12K Flex system. QuantStudio 12K Flex OpenArray速 plates are microscope slidesized and are arranged in 48subarrays of 64 through-holes, with a total of 3,072through-holes for individual 33nL reactions (Figure 3). Plates are coated with hydrophilic and hydrophobic compounds to retain reagents in through-holes via surface tension. Subarray Through-holes retain 33 nL reaction mixtures via surface tension Hydrophobic Hydrophilic Figure 3. OpenArray速 plate technology. Figure 2. The QuantStudio 12K Flex AccuFill System simplifies sample loading into QuantStudio 12K Flex OpenArray速 Plates. 5
  • 6. Flexibility No compromises necessaryfull compatibility with diverse applications, plus single-tube to 12,000 reaction/run throughput Five interchangeable blocks. Choose and load the thermal-cycling block you need for the experiment at hand. Additional blocks are available to scale your throughput capability to up to 12,000 data points in a single run. Provides a seamless switch from qPCR to digital PCR. Increase the precision and sensitivity of your experiments by switching from real- time PCR to digital PCR mode using QuantStudio digital PCR kits and DigitalSuite Software with the QuantStudio OpenArray速 block. From late Ct values to undetermined copy numbers, digital PCR provides a method to rescue ambiguous qPCR results, increasing the success rate of your experiments. Multiplexing flexibility. With up to six independent excitation and emission filter channels, the QuantStudio 12K Flex system flexibly accommodates your real-time chemistry needs and provides ample multiplexing capability. Dual-color detection is enabled for QuantStudio 12K Flex OpenArray速 plates. Impressive throughput without complicated robotics. With 384-well TaqMan速 Array Cards, theres no need for liquid-handling robotics or complex pipetting to load; simply add your sample and master mix and run on the QuantStudio 12K Flex system. Its often the unexpected results in the lab that lead to the important breakthroughs. With this one instrument, your lab can be ready for many types of experiments and users, from low- to high-throughput sample processing and virtually any PCR application that requires fluorescence detection. 6
  • 7. Figure 4. Real-time PCR reproducibility and 9-log dynamic range. Amplification plot shows results from real-time PCR of 18S DNA in 10-fold dilutions starting at 25,000 copies using the 384-well block. The data show highly reproducible results for input target quantities cover- ing nine orders of magnitude (9 logs). These data illustrate the broad dynamic range of the system. Cycle 24 4020161210 388 364 6 342 32302826221814 Rn 0.1 1 10 0.01 Enhanced OptiFlex速 System The QuantStudio 12K Flex Real-Time PCR System uses a white light LED optics system enabling powerful, extremely accurate and sensitive data collection (Figure 4). Additionally, the white light LED provides a broad spectrum of light for maximum resolution of 12,000 data points. The Enhanced OptiFlex速 System can also capture QuantStudio 12K Flex OpenArray速 plate identities from their barcodes. You can instantly load the appropriate experiment files for one-touch runs to help ensure that the plates were loaded correctly. 7
  • 8. Take on the experiments youve always wanted to do, the QuantStudio 12K Flex system has the capabilities youll need to follow your research leads with ease. Start your project with lower-throughput feasibility experiments and then scale up to get statistically significant answers for publishing groundbreaking results. Quick, simple block changes. Thermal blocks are easy to change for all users in the laband no tools or service calls are needed. In just minutes, you can get the QuantStudio 12K Flex system ready for experiments requiring just a few data points per runor thousands. Ample data storage. Know that your data are always secure. The QuantStudio 12K Flex system can store data from over 100 runs without an external database, so your productivity wont be limited by data storage concerns. Fully compatible with TaqMan速 Assay formats and chemistry. As you scale your experiments and increase throughput, be assured that TaqMan速 Assay sensitivity, specificity, and wide dynamic range are optimized across all QuantStudio 12K Flex system block formats. Automation capability. With the QuantStudio 12K Flex Twister速 II Automation Robot (Figure 5), you can automate your real-time PCR runs to accommodate sample throughput increases. Scalability A single instrument for your real-time PCR needs today with the capabilities youll need tomorrow Figure 5. The QuantStudio 12K Flex System can be fully integrated with the Twister速 II Robot for real-time PCR in automated environments. 8
  • 9. Single-tube TaqMan速 Assays 384-well TaqMan速 Array Cards TaqMan速 Assays in 96- and 384-well plates QuantStudio 12K Flex OpenArray速 plates 9
  • 10. QuantStudio 12K Flex Software User-friendly Feature-rich touch screen interface makes it easy to get started and keep going (Figure 6). Intuitive interface. Easily identifiable icons on the touch screen guide you through the workflow to set up runs and analyze experiments. The graphical interface makes it easy to edit thermal cycling conditions for your 96-well, 384-well, and TaqMan速 Array Card blocks, and enables one-touch runs for the OpenArray速 block. Sample tracking from start through analysis. The OpenArray速 SampleTracker helps you take your samples from 96-well plate format, to visually planning your OpenArray速 plate configuration, to the AccuFill Software for loading your OpenArray速 plates, and finally integrates with QuantStudio 12K Flex Software for one-touch runs. Real-time monitoring. Monitor up to 15 instruments simultaneously and view amplification plots from subarrays. A data analysis suite at your fingertips Innovative software applications to analyze your data. Enhanced gene expression analysis. ExpressionSuite Software v1.0 can analyze data from small or large projects, even 100+ runs. Quickly check data quality and perform sophisticated statistical analysis to understand expression patterns and relationships between samples. Real-time control for genotyping cluster analysis. You can optimize your genotyping run times using a real-time PCR progress monitor to determine the ideal cycle for cluster analysis. Straightforward transition to digital PCR. With DigitalSuite Software v1.0 you can increase the precision and sensitivity of your qPCR experiments by switching to digital PCR mode (Figure 7). Digital PCR provides new capabilities to help you get more from your experiments. 10
  • 11. Figure 6. Easy analysis of OpenArray速 runs: toggle between targets, samples, and subarray. High-throughput capable Easily keep track of your samples and your results High-throughput experiment setup. Create multiple experiment files simultaneously with the push of a single button and match them with your unique sample files. You can import your plate setup files from other Applied Biosystems速 real-time systems to facilitate experiment setup. Easy, automated data export. Set up experiment templates with export preferences that direct the system software to auto-export your results to a file or email them to you. LIMS compatibility. Open application program interfaces (API) allow integration with third-party systems such as LIMS (laboratory integration management systems) or custom automated platforms. The optional 21 CFR Part 11 compliance module assists with security, auditing, and e-signature records for data traceability. Figure 7. Analyze your digital PCR results using DigitalSuite Software for absolute quantitation with greater sensitivity and precision of rare- target detection. 1/400 1/200 1/100 1/10 0.039copies/rxn 0.062copies/rxn 0.122copies/rxn 1.210copies/rxn 0.059copies/rxn 0.116copies/rxn 0.286copies/rxn 3.060copies/rxn 0.097copies/rxn 0.165copies/rxn 0.362copies/rxn 3.508copies/rxn 0.1 1 10 100 Concentration (copies/nL) 1 10 100 Concentration (copies/nL) 1 10 100 1000 Concentration (copies/nL) 11
  • 12. Applications & technologies A wide range of TaqMan速 Assays offer superior performance for both routine and challenging applications Gene expression analysis. TaqMan速 Gene Expression Assays are the most comprehensive set of quantitative gene expression assays available, providing more than 1.3 million primer/probe sets for 23 species, in four sizes, including your choice of FAM or VIC速 dye labels. Custom assays are available for studying the expression of any gene or splice variant in any organism. SNP genotyping. The precision of TaqMan速 probebased chemistry makes SNP genotyping studies easy. Choose from over 4.5 million predesigned human and mouse TaqMan速 SNP Genotyping Assays and 2,700 TaqMan速 DME Genotyping Assays, or Custom TaqMan速 SNP Genotyping Assays, in various sizes. Copy number analysis. TaqMan速 Copy Number Assays are a collection of over 1.6 million predesigned assays with genome-wide coverage for human, over 180,000 assays targeting exons for mouse, and Custom Plus and standard Custom TaqMan速 Copy Number Assays when a predesigned assay is not readily available. Digital PCR technology. Ideal for sensitive detection of rare targets such as in cancer mutations, viruses, and GMO detection. In digital PCR, reactions are split into hundreds to thousands of mini-reactions to provide a digital readout of real-time PCR results. castPCR technology. TaqMan速 Mutation Detection Assays are designed to detect and measure DNA mutations with extremely high specificity against a background of wild- type genomic DNA (gDNA). These assays are powered by competitive allele-specific TaqMan速 PCR technology, known as castPCR technology, for detection of mutations present at less than 0.1% in gDNA. MicroRNA and other noncoding RNA analysis. Innovative TaqMan速 Assays and tools are available for long noncoding RNA quantification, miRNA profiling, pri-miRNA quantification, small RNA quantification, and targeted miRNA quantification. High resolution melt (HRM) analysis. HRM analysis is a rapid, economical method for mutation scanning and methylation analysis in genomic DNA. 12
  • 13. Pharmacogenomics panels. For researchers involved in pharmacogenomics, our fixed-content panel of selected TaqMan速 DME assays is ideal for high-throughput analysis of the effects of genes on human responses to drugs. This panel contains all known high-value DME pharmacogenetics markers, including PharmaADME core markers. Barcoding panels. For high-throughput tracking and confirmation of sample identity and gender within a large biorepository setting, our fixed-content OpenArray速 panels contain a defined set of selected autosomal and Y chromosome SNP markers common to commercial arrays. Gene expression pathway panels. For researchers performing disease studies, these fixed-content gene expression OpenArray速 Pathway Panels are for high- throughput analysis of commonly studied, disease-related gene families. These include human cancer, kinome, inflammation, signal transduction, and stem cell panels, and mouse inflammation panels. Innovative assay panels to take your research to the next level Life Technologies research and development scientists continue to develop new collections of TaqMan速 Assays to use with Applied Biosystems速 real-time PCR technology. Most recently weve introduced the following novel products: 13
  • 14. Description Specification Block configurations and volumes 96-well (10100 袖L reactions), Fast 96-well (1030 袖L reactions) 384-well (520 袖L reactions) TaqMan速 Array Cards (~1 袖L reactions) OpenArray速 Plates (33 nL reactions) Block change design Block change from front in less than 1 min; no tools or service call required Excitation source Enhanced OptiFlex速 system, white LED Instrument control Instrument touch screen, networked computer, or attached computer Detection channels Decoupled: 6 emission, 6 excitation (96-well/Fast, 384-well, TaqMan速 blocks) Coupled: 4 emission, 4 excitation (OpenArray速 blocks) 21 CFR p11 enablement Optional software module Dimensions (W x D x H) 50.5 cm x 67.2 cm x 73.8 cm Weight 69kg(152lb) Power 100240 V Remote monitoring Available to monitor up to 15 networked instruments simultaneously Data export format User configurable: *.xls, *xlsx, *.txt 96-well, 96-well Fast, 384-well, TaqMan速 Array Card blocks OpenArray速 block Detection channels Decoupled: 6 emission, 6 excitation Coupled: 4 emission, 4 excitation Well-to-well variability 賊 0.25属C 賊 0.75属C Max block ramp rate 3.0属C/sec (384-well) 3.0属C/sec Run time 30 min expected (Fast 96-well block) 35 min (384-well block, using Fast master mix) 2 hr (gene expression) 4 hr (genotyping) Demonstrated sensitivity To 1 copy To 1 copy Dynamic range To 9 logs To 7 logs Resolution As low as 1.5-fold change for singleplex reaction As low as 2-fold change for singleplex reaction Hardware specifications QuantStudio 12K Flex Real-Time PCR System 14
  • 15. Software Description Blocks supported Embedded in QuantStudio 12K Flex Software v1.0 SampleTracker v1.0 Software Facilitate sample tracking from 96-well to 384-well plates Illustrates mapping to OpenArray速 plates QuantStudio 12K Flex OpenArray速 block HRM Software Module for QuantStudio 12K Flex system High resolution melt analysis 96-well, 384-well blocks 21 CFR Part 11 Software Module for QuantStudio 12K Flex system Quality control system compliance features All blocks Downstream applications accessible from QuantStudio 12K Flex software home screen DigitalSuite Software Digital PCR analysis that enables duplex analysis, one-click launch, amplification curves, and heat maps All QuantStudio 12K Flex system blocks ExpressionSuite Software Gene expression analysis software supporting up to 100 files with enhanced performance time. The software features relative quantification with p-value, correlation plots, box plots, heat maps, and volcano plots All QuantStudio 12K Flex system blocks TaqMan速 Genotyper Software Improved version of existing software product that includes single-plate views All QuantStudio 12K Flex system blocks Software specifications Download from www.appliedbiosystems.com 15
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  • 17. Kevin Munelly, Life Technologies The highly flexible QuantStudio 12K Flex system allows you to perform the experiments that were difficult in the past. 17
  • 18. Applied Biosystems速 service plans offer: Highly valued field services Protect your technology investment with a wide range of service plans that fit your laboratory requirements and budget needs. Highly trained, certified service engineers use the latest technology to quickly diagnose and fix your instruments. Realize up to 45% savings on instrument care through purchase of a service plan. Award-winning remote services Remotely and proactively monitor, alert, diagnose, and correct your instrument performance for maximum uptime. Avoid unplanned interruptions in lab operations with automatic alerts sent to your field service engineer (FSE). Remotely track critical system parameters with real-time reporting. Compliance and validation services Broad range of audit-quality services, including installation qualification (IQ), operation qualification/instrument performance verification (OQ/IPV), pure dye calibration, on-site temperature verification, computer system validation, and risk assessment. Comprehensive packages with rigorously executed protocol documentation including all the data collected. A partnership with you that utilizes the latest technology to move quickly into testing and production using audit-quality expert support to minimize the frustration and burden of developing and maintaining compliance and change control. Applied Biosystems速 service plans Applied Biosystems速 service & support AB Complete AB Assurance AB Maintenance Repair response time (business days) Guaranteed next day: on-site repairs Guaranteed 2-day: on-site repairs Target 2-day: remedial repairs Remote instrument monitoring and diagnostics + + Priority phone and email access to instrument support + + Priority phone and email access to application technical support + + + Instrument operating software upgrades + Instrument qualification services + Planned maintenance + + + Parts, labor, and travel Included Included Discount with Plus plan 18
  • 19. Instrument systems (with desktop computer) Cat. No. QuantStudio 12K Flex Real-Time PCR System with OpenArray速 Block (includes AccuFill system) 4471090 QuantStudio 12K Flex Real-Time PCR System with OpenArray速 Block (without AccuFill system) 4472380 QuantStudio 12K Flex Real-Time PCR System with TaqMan速 Array Card Block 4471089 QuantStudio 12K Flex Real-Time PCR System with 384-Well Block 4471134 QuantStudio 12K Flex Real-Time PCR System with 96-Well Fast Block 4471088 QuantStudio 12K Flex Real-Time PCR System with 96-Well Block 4471087 Additional thermal cycling blocks and accessories 96-Well Block Upgrade Kit 4453543 96-Well Fast Block Upgrade Kit 4453544 384-Well Block Upgrade Kit 4453545 TaqMan速 Array Card Block Upgrade Kit 4453546 OpenArray速 Block Upgrade Kit with AccuFill System 4471067 QuantStudio 12K Flex AccuFill Upgrade Kit (For existing AccuFill system users) 4471022 QuantStudio 12K Flex Twister 速 II Automation Robot 4471066 Applied Biosystems速 Service Plans Applied Biosystems速 Complete Service Plan Contact us Applied Biosystems速 Assurance Service Plan Contact us Applied Biosystems速 Maintenance Service Plan Contact us Ordering information 19
  • 20. lifetechnologies.com For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 息 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan速 is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Twister is a registered trademark of Caliper Life Sciences Inc. Printed in the USA. CO01523 1211 For more information about the QuantStudio 12K Flex Real-Time PCR System or any of our real-time PCR products, contact your Life Technologies representative or visit www.appliedbiosystems.com/quantstudio