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Collection of fish blood

Naveen Rajeshwar B
Naveen Rajeshwar B

Different methods of fish blood collection followed in aquaculture and aquatic animal health management. Basics of clinical pathology study in fish. Blood-Serum-Plasma.

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Collection of Fish Blood Submitted by Submitted to B.Naveen Rajeshwar Dr TJ Abraham Dept of AAH Dept of AAH
Introduction Blood constitute 5% of total body weight. The regular monitoring of fish blood is a very useful diagnostic tool in establishing the health status of the fish farm stocks. There are several different techniques which can be used to collect blood samples from fish. The presence of humoral antibody and cellular cytophilic antibody can be assessed from blood serum or plasma.
Different methods of Blood Collection Tail ablation of euthanized fish Caudal vein Puncture Cardiac Puncture Dorsal Aorta Puncture
Equipment's Required Fish Anaesthetising agent (MS 222) Sterile needle Syringe or vacutainer tube Dip net Gloves Thermometer Soft sponge or mat Disinfectant Sterile knife for tail ablation Empty tubes
Tail Ablation This sampling technique is suitable for smaller fish (<10 cm). Small fishes have small blood vessels and very low volume of blood. Sometimes, its difficult to collect blood efficiently by this, method due to rapid coagulation. In this procedure, the fish is killed or sacrificed by administering with an anaesthetic solution. Cut dorso-ventrally through the Caudal peduncle. Wipe the caudal area with absorbent tissue to avoid contamination with mucus and water
Place the heparinised collection tube or capillary tube at the end of caudal vessel which you have just cut. In case of capillary tube allow the tube to fill by means of capillary action.
Caudal Vein Puncture This method which can be used to repeatedly to take blood samples from larger fish ( usually >10 cm long). From 0.5 to 1 ml blood can be drawn from fish of 200g every week without causing much mortalities. Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. Insert the needle on the mid- ventral line behind the anal fin at an angle of 45. Push the needle into the musculature until the spinal column is reached
Keeping the steady vacuum in the syringe, slowly withdraw the needle until blood enters the syringe. Remove the needle from the fish completely and empty the contents of the syringe into a tube held on ice. Return the fish to the recovery tank and monitor the recovery.
Cardiac Puncture This method which can be used to take blood samples from larger fish (usually >10 cm long). Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. Hold the fish with ventral side on top. Insert the needle vertically, midway between the anterior base of pectoral fins. Keeping the steady vaccum in the syringe, slowly withdraw the needle until blood enters the syringe.
Slowly remove the needle from the fish completely. Remove the needle and empty the contents of the syringe into a tube held on ice.
Dorsal Aorta Puncture This method which can be used to repeatedly to take blood samples from larger fish ( usually >10 cm long). Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. The needle is inserted midway between posterior base of anal fin and the beginning of the caudal fin. When the needle is stopped by the backbone, the aorta will be penetrated and the blood will flow into the syringes.
Slowly remove the needle from the fish completely. Remove the needle and empty the contents of the syringe into a tube held on ice
Vacutioner tubes- Types Red Top- No additives. Used for serum collection. Green Top- Anticoagulant Heparin. Used for plasma collection. Purple Top- Anticoagulant EDTA. Used for Haematology purposes. Grey Top- Additive Sodium Fluoride. Used for Glucose measurements. Light Blue top- Additive Sodium Citrate. Used for Coagulation Studies
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Collection of fish blood

  • 1. Collection of Fish Blood Submitted by Submitted to B.Naveen Rajeshwar Dr TJ Abraham Dept of AAH Dept of AAH
  • 2. Introduction Blood constitute 5% of total body weight. The regular monitoring of fish blood is a very useful diagnostic tool in establishing the health status of the fish farm stocks. There are several different techniques which can be used to collect blood samples from fish. The presence of humoral antibody and cellular cytophilic antibody can be assessed from blood serum or plasma.
  • 3. Different methods of Blood Collection Tail ablation of euthanized fish Caudal vein Puncture Cardiac Puncture Dorsal Aorta Puncture
  • 4. Equipment's Required Fish Anaesthetising agent (MS 222) Sterile needle Syringe or vacutainer tube Dip net Gloves Thermometer Soft sponge or mat Disinfectant Sterile knife for tail ablation Empty tubes
  • 5. Tail Ablation This sampling technique is suitable for smaller fish (<10 cm). Small fishes have small blood vessels and very low volume of blood. Sometimes, its difficult to collect blood efficiently by this, method due to rapid coagulation. In this procedure, the fish is killed or sacrificed by administering with an anaesthetic solution. Cut dorso-ventrally through the Caudal peduncle. Wipe the caudal area with absorbent tissue to avoid contamination with mucus and water
  • 6. Place the heparinised collection tube or capillary tube at the end of caudal vessel which you have just cut. In case of capillary tube allow the tube to fill by means of capillary action.
  • 7. Caudal Vein Puncture This method which can be used to repeatedly to take blood samples from larger fish ( usually >10 cm long). From 0.5 to 1 ml blood can be drawn from fish of 200g every week without causing much mortalities. Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. Insert the needle on the mid- ventral line behind the anal fin at an angle of 45. Push the needle into the musculature until the spinal column is reached
  • 8. Keeping the steady vacuum in the syringe, slowly withdraw the needle until blood enters the syringe. Remove the needle from the fish completely and empty the contents of the syringe into a tube held on ice. Return the fish to the recovery tank and monitor the recovery.
  • 9. Cardiac Puncture This method which can be used to take blood samples from larger fish (usually >10 cm long). Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. Hold the fish with ventral side on top. Insert the needle vertically, midway between the anterior base of pectoral fins. Keeping the steady vaccum in the syringe, slowly withdraw the needle until blood enters the syringe.
  • 10. Slowly remove the needle from the fish completely. Remove the needle and empty the contents of the syringe into a tube held on ice.
  • 11. Dorsal Aorta Puncture This method which can be used to repeatedly to take blood samples from larger fish ( usually >10 cm long). Render the fish unconscious in an anaesthetic solution. Flush out the syringe with Anticoagulant like Heparin solution. The needle is inserted midway between posterior base of anal fin and the beginning of the caudal fin. When the needle is stopped by the backbone, the aorta will be penetrated and the blood will flow into the syringes.
  • 12. Slowly remove the needle from the fish completely. Remove the needle and empty the contents of the syringe into a tube held on ice
  • 13. Vacutioner tubes- Types Red Top- No additives. Used for serum collection. Green Top- Anticoagulant Heparin. Used for plasma collection. Purple Top- Anticoagulant EDTA. Used for Haematology purposes. Grey Top- Additive Sodium Fluoride. Used for Glucose measurements. Light Blue top- Additive Sodium Citrate. Used for Coagulation Studies