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Dr. Stickle

an EST-Database
Dr. Stickle
an EST-Database
The Goal
The Goal


 Creating a pipeline for EST-Analysis
The Goal


 Creating a pipeline for EST-Analysis
 Displaying the results via an online
 framework
wtf is a pipeline?
Different steps of analysis performed in an
             automated fashion




       wtf is a pipeline?
wtf is a pipeline?


Different steps of analysis performed in an
             automated fashion
Analysis:
Assembly of EST-Reads into contigs
SNP-Detection
               MIRA
               But:
       Takes ages
       not well documented
       buggy
Assembly of EST-Reads into contigs
SNP-Detection
               MIRA
               But:
       Takes ages
       not well documented
       buggy
Assembly of EST-Reads into contigs
SNP-Detection
               MIRA
               But:
       Takes ages
       not well documented
       buggy
MIRA
Assembly of EST-Reads into contigs
SNP-Detection


               But:
       Takes ages
       not well documented
       buggy
MIRA
Assembly of EST-Reads into contigs
SNP-Detection


               But:
       Takes ages
       not well documented
       buggy
MIRA
Assembly of EST-Reads into contigs
SNP-Detection

               But:
       Takes ages
       not well documented
       buggy
MIRA
Assembly of EST-Reads into contigs
SNP-Detection

               But:
       Takes ages
       not well documented
       buggy
SNPs   ORFs   Contigs BLAST
               MIRA




       PFAM        BLAST2GO
MIRA




SNPs   ORFs   Contigs BLAST




       PFAM           BLAST2GO
MIRA
              Contigs




SNPs   ORFs             BLAST




       PFAM        BLAST2GO
MIRA
              Contigs




SNPs   ORFs             BLAST




       PFAM        BLAST2GO
MIRA
              Contigs




SNPs   ORFs             BLAST




       PFAM        BLAST2GO
MIRA
              Contigs




SNPs   ORFs             BLAST




       PFAM        BLAST2GO
MIRA
              Contigs




SNPs   ORFs             BLAST




       PFAM        BLAST2GO
BLAST
Basic Local Alignment Search Tool
BLAST
Basic Local Alignment Search Tool
BLAST
              Basic Local Alignment Search Tool




 Standard for searching sequences against
 a database
BLAST
            Basic Local Alignment Search Tool




 Standard for searching sequences against
  a database
 emphasizes speed over sensitivity
BLAST
            Basic Local Alignment Search Tool




 Standard for searching sequences against
  a database
 emphasizes speed over sensitivity
BLAST
            Basic Local Alignment Search Tool




 Standard for searching sequences against
  a database
 emphasizes speed over sensitivity
BLAST
            Basic Local Alignment Search Tool




 Standard for searching sequences against
  a database
 emphasizes speed over sensitivity
Tools


                   gene ontology
Blast2GO
                     mapping


              open reading frame
  ORF
                  prediction


 PFAM          domain annotation
Blast2GO
Tools


                   gene ontology
Blast2GO
                     mapping


              open reading frame
  ORF
                  prediction


 PFAM          domain annotation
ORF
Tools


                   gene ontology
Blast2GO
                     mapping


              open reading frame
  ORF
                  prediction


 PFAM          domain annotation
PFAM
Dr. Stickle
Ugly *.ace-output generated via MIRA
What weve got here:
What weve got here:

Different tools
many different output-files
What weve got here:

     Different tools
     many different output-files

             What we want:

a structured database containing all the
              information
How to parse


Class 束Parser損
 Function BLAST-Parser
 Function PFAM-Parser
 Function FASTA-Parser
 ...
                                         Data



Script
 read input
 use parser
 insert db
How to parse


Class 束Parser損
 Function BLAST-Parser
 Function PFAM-Parser
 Function FASTA-Parser
 ...
                                         Data



Script
 read input
 use parser
 insert db
How to parse


Class 束Parser損
 Function BLAST-Parser
 Function PFAM-Parser
 Function FASTA-Parser
 ...
                                         Data



Script
 read input
 use parser
 insert db
How to parse


Class 束Parser損
 Function BLAST-Parser
 Function PFAM-Parser
 Function FASTA-Parser
 ...
                                         Data



Script
 read input
 use parser
 insert db
How to parse

                                Data
Class 束Parser損
 Function BLAST-Parser
 Function PFAM-Parser
 Function FASTA-Parser
 ...            Script
                  read input
                  use parser
                  insert db


                           Database
>abc_123
agtagtacgtacgtggacgtatgact
>def_456
agtagtacgtacgtggacgtatgact
Dr. Stickle
Dr. Stickle
Dr. Stickle
Summary & Results
Summary & Results

created the pipeline
analysed data
started filling the database
Summary & Results

created the pipeline
analysed data
started filling the database

        To be done


wait for MIRA
SNP-parser
thx to:


Marvin, for 束time till scooter損 and sending us to Lothar
Lothar, for providing always friendly and calm advice
Suse, for actually having used MIRA at least once
Andrew, for Andreas
Andreas, for Andrew
Bastien Chevreux, for not fixing those damn bugs in MIRA
k,thx,bai
Dr. Iglo
Dr. Iglo
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