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DSM eBOOST, Industrially Proven Yeast Technology

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Phillip Bureman
Phillip Bureman

This document discusses DSM's yeast technologies for ethanol production. It introduces several DSM sales and technical representatives. DSM offers two yeast products, eBOOST and eBOOST GT, which can increase ethanol yields by 3% on average compared to standard yeast. These yeasts also reduce glycerol production by up to 68% and decrease levels of contaminants like acetaldehyde. Commercial trial data shows higher ethanol levels and yields from eBOOST yeast. Charts are presented comparing eBOOST GT to a competitor's yeast showing eBOOST GT's higher sugar consumption and ethanol tolerance.

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CONFIDENTIAL Advanced Yeast Technologies 息 2020 DSM. All rights reserved. Same Corn, More Value: Industrially proven Yeast Technology Phil Bureman Sales Manager DSM Bio-Products & Services Phil.Bureman@DSM.com Cell: +1 913-708-4969 September 11, 2020
Page CONFIDENTIAL Confidential Phil Bureman Sales Manager Started in biofuels industry in 1993 25 yrs Technical Sales at Nalco with focus on process chemicals and fuel quality 10+yrs RFA Technical Committee Began with DSM in March 2020 Our Local Team DSM Biobased Product & Services Michael Mszal Bioprocess and Mfg Specialist Started in biofuels industry in 2014 8 years experience in production, R&D, scale-up, and other roles in various fermentation houses as a supervisor and process development engineer Began with DSM in May 2016 1 Pat Moran Sales Manager Started in biofuels industry in 2005 Held various positions with Walling Water/ HOH Water, US Water including General Manager/President, Regional Sales Manager Began with DSM in August 2019 Who is DSM? Arvind Kaul Bioprocess and Mfg Specialist Started in biofuels industry in 2016 Held technical positions with Roche Vitamins, Novozymes, Genomatica, DSM Overall 25+ years of technical experience in fermentation based processes. Began with DSM in late May 2016
Page CONFIDENTIAL Confidential Our purpose is to create brighter lives for people today and generations to come We connect our unique competences in Life Sciences and Materials Sciences to create solutions that nourish, protect and improve performance DSM: A Global Innovation and Sustainability Leader #2 currently on Forbes Sustainability Index 2 Animal Nutrition & Health Human Nutrition & Health; Food Specialties; Personal Care Materials Sustainability Leader Fortune Magazine: 2nd in Change the World index Intrinsically Innovative 21% sales from innovation products Global presence 22,174 employees worldwide Strong performance $10 billion sales up 2% from 2018 Other PEOPLE PLANET - PROFIT What can DSM offer to your site?
Page CONFIDENTIAL Confidential DSM in biofuels 150 years of yeast experience Vision: Accelerate the growth of biofuels as global fuel source Mission: Deliver the technologies and expertise that enhance biofuel production History: A rich history in ethanol production; began as Gist-Brocades producing yeast and spirits since 1869 150 years Gist-Brocades, NL Elgin, IL We are a relative new comer in USA Bioethanol so we must Innovate! Let me show you what we have created so far
Page CONFIDENTIAL Confidential Page 4 DSM technology in biofuels Ethanol industry - Corn process Pretreatment Enzymatic hydrolysis Fermentation Distillation Corn stover Ethanol Saccharification Fermentation Distillation Corn kernel Ethanol Corn mash Corn fiber Pretreatment & hydrolysis Fermentation DDGS Corn oil 1.5G EtOH 1G EtOH 2G EtOH Corn stover Project Liberty Todays discussion will focus on our Yeast products, but. Adv. Yeast
Page CONFIDENTIAL Confidential Why Should You Consider DSM BP&S We are the newcomer to the USA Ethanol Industry So we have to innovate and work harder to earn and keep your business! DSM DOES NOT charge Technology or Ferm Fees All In $/kg Pricing! DSM Yeast produces a Cleaner Ethanol! DSM Yeast delivers an average of 3% more ethanol compared to non GMO industry standard with the same or lower amounts of acetaldehyde & acetal & other fusels Glycerol reduction (up to 68%) Lower energy costs by increasing drying and evaporator efficiency Improves whole stillage separation efficiency & corn oil recovery We believe our nitrogen uptake pathway converts more carbon to useful amino acids & polypeptides thereby improving the quality of your DDGS We offer Monthly Lab Analysis, Quarterly Business Reviews & Regular Operator Training. We are MORE than a delivery & an invoice! 5
Page CONFIDENTIAL Confidential DSM Yeast has very good acid tolerances showing up to 0.5% LA + 0.2% AA with negligible inhibition to finish DSM Yeast DO NOT contribute to the production of Acetic or Lactic acids eBoostTM GT offers high GA reductions (~50%) compared to traditional yeasts, and further increases shown over other GA expressing yeasts A recent mini-ferm trial showed additional 25% GA reduction eBOOST & eBOOST GT are produced in the U.S. / All regulatory approvals in place (MCAN*, GRAS) At this time we offer only Active Dry Yeast (ADY) 6 I can share some Performance Data but first Lets talk about what is important to you *Microbial Commercial Activity Notices (MCANs) Why Should You Consider DSM BP&S
Page CONFIDENTIAL Confidential 7 Lets focus on your interest What is most important to you when choosing a yeast supplier? What are your expectations from a yeast supplier? What do you wish a yeast supplier would offer or innovate? Other thoughts/questions/coaching for us? Are you making or considering making hand sanitizer? Do you have an ability to test our yeast in a mini ferm or lab trial? If so, do you place much value is such tests? Is there an appetite now to trial one or both of our yeast? Have you had challenges optimizing your plant to a new yeast in the past? If so, what expectations do you have for DSM in a potential trial or switch? We are offering very low cost trials at this time!
Page CONFIDENTIAL Confidential DSM eBOOST yields CLEAN Ethanol Lab Test Measurements in Fermentation Broth 8 Mash Yeast Acetaldehyde (mg/l) % of ER Corn Mash #1 Ethanol Red 77.5 100 Corn Mash #1 eBOOST 65.9 85 Corn Mash #2 Ethanol Red 101 100 Corn Mash #2 eBOOST 73.6 73 Mash Yeast (Acetal) 1,1-dieth oxyethane (mg/l) % of ER Corn Mash #1 Ethanol Red 6.77 100 Corn Mash #1 eBOOST 7.56 112 Corn Mash #2 Ethanol Red 10.9 100 Corn Mash #2 eBOOST 8.21 75 Temperature Profile for this testing was SOF to 22 Hrs: @ 94 F 22 Hrs to 36 Hrs @ 92.5 F 36 Hrs to 69 Hrs (EOF) @ 89.6 F Measurements were done from fermentation drop samples Drop samples were centrifuged and supernatants collected and frozen Samples sent to a certified third-party analytical lab for acetaldehyde and 1,1-Diethoxyethane (acetal) analysis by GC Lets talk Commercial Results
Page CONFIDENTIAL Confidential Commercial trial results with Same corn, more value eBOOST compared to market standard: About 3% avgerage EtOH yield improvement Down to 68% glycerol reduction 32% 45% 38% 50% 40% 41% 38% 41% 0% 20% 40% 60% 80% 100% Market standard Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average Low glycerol levels eBOOST 2.0% 5.0% 4.7% 3.5% 2.1% 1.9% 2.5% 3.1% 98% 100% 102% 104% 106% Market standard Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average EtOH yield improvement eBOOST Equal or very similar performance observed with eBOOST GT In todays low price market your plant could make the same ethanol with less corn input cost! How does DSM compare with other Market Leaders?
Page CONFIDENTIAL Confidential Page 10 Outperforms competitors Best yield potential in the market 2.5 - 5% 50 - 60% Equal or very similar performance observed with eBOOST GT Let me share details on in Plant Performance Data for eBoost GT Vs a Competitor Advanced Yeast
Page CONFIDENTIAL Confidential Charts at Fermentation drop Trial-O-Rama eBOOST delivers higher EtOH and EtOH/Liq solids at drop 11 息 2019 DSM. All rights reserved. eBOOST delivers consistently higher EtOH titers and yields at drop vs. the other two strains. Compt 1 - Baseline Compt 2 eBOOST Compt 2 eBOOST Ethanol Ethanol/Liq solids Compt 1 Re-baseline Compt 1 - Baseline Compt 1 Re-baseline
Page CONFIDENTIAL Confidential eBOOST GT Recipe Key Operating Conditions 12 Propagator Liq solids ~ 20%; Yeast Pitch 1 g/l with opportunity for optimization. Urea ~ 1 g/l No GA Propagation time of ~ 8-9 hrs Temperature: 90-93 F Main Fermenter Mash solids of 32-34% Urea ~1.0- 0.8 g/l 50% of normal GA dosage Plant standard temperature staging 92 88 F targeted ranges; In sustained commercial use, eBOOST GT had good finish with high ethanol titers even when fermentation temperatures occasionally peaked at 95F First, let me share some sugar consumption data vs a competitor using this recipe
Page CONFIDENTIAL Confidential 13 eBOOST GT vs. Comp Yeast - Sugar Profiles @ Recent Ferm Trial Equal or very similar performance observed with eBOOST
Page CONFIDENTIAL Confidential 14 Evaluation of performance eBOOST GT vs. Comp Yeast @ Recent Ferm Trial This data is @ a 50% reduction in GA which can be optimized up to 70%. eBOOST GT has a higher tolerance for glucose peak compared to competitor yeast eBOOST GT produces less Glycerol during fermentation No significant difference in lactic and acetic acid production compared to current plant levels. Equal or very similar performance observed with eBOOST
DSM eBOOST, Industrially Proven Yeast Technology

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DSM eBOOST, Industrially Proven Yeast Technology

  • 1. CONFIDENTIAL Advanced Yeast Technologies 息 2020 DSM. All rights reserved. Same Corn, More Value: Industrially proven Yeast Technology Phil Bureman Sales Manager DSM Bio-Products & Services Phil.Bureman@DSM.com Cell: +1 913-708-4969 September 11, 2020
  • 2. Page CONFIDENTIAL Confidential Phil Bureman Sales Manager Started in biofuels industry in 1993 25 yrs Technical Sales at Nalco with focus on process chemicals and fuel quality 10+yrs RFA Technical Committee Began with DSM in March 2020 Our Local Team DSM Biobased Product & Services Michael Mszal Bioprocess and Mfg Specialist Started in biofuels industry in 2014 8 years experience in production, R&D, scale-up, and other roles in various fermentation houses as a supervisor and process development engineer Began with DSM in May 2016 1 Pat Moran Sales Manager Started in biofuels industry in 2005 Held various positions with Walling Water/ HOH Water, US Water including General Manager/President, Regional Sales Manager Began with DSM in August 2019 Who is DSM? Arvind Kaul Bioprocess and Mfg Specialist Started in biofuels industry in 2016 Held technical positions with Roche Vitamins, Novozymes, Genomatica, DSM Overall 25+ years of technical experience in fermentation based processes. Began with DSM in late May 2016
  • 3. Page CONFIDENTIAL Confidential Our purpose is to create brighter lives for people today and generations to come We connect our unique competences in Life Sciences and Materials Sciences to create solutions that nourish, protect and improve performance DSM: A Global Innovation and Sustainability Leader #2 currently on Forbes Sustainability Index 2 Animal Nutrition & Health Human Nutrition & Health; Food Specialties; Personal Care Materials Sustainability Leader Fortune Magazine: 2nd in Change the World index Intrinsically Innovative 21% sales from innovation products Global presence 22,174 employees worldwide Strong performance $10 billion sales up 2% from 2018 Other PEOPLE PLANET - PROFIT What can DSM offer to your site?
  • 4. Page CONFIDENTIAL Confidential DSM in biofuels 150 years of yeast experience Vision: Accelerate the growth of biofuels as global fuel source Mission: Deliver the technologies and expertise that enhance biofuel production History: A rich history in ethanol production; began as Gist-Brocades producing yeast and spirits since 1869 150 years Gist-Brocades, NL Elgin, IL We are a relative new comer in USA Bioethanol so we must Innovate! Let me show you what we have created so far
  • 5. Page CONFIDENTIAL Confidential Page 4 DSM technology in biofuels Ethanol industry - Corn process Pretreatment Enzymatic hydrolysis Fermentation Distillation Corn stover Ethanol Saccharification Fermentation Distillation Corn kernel Ethanol Corn mash Corn fiber Pretreatment & hydrolysis Fermentation DDGS Corn oil 1.5G EtOH 1G EtOH 2G EtOH Corn stover Project Liberty Todays discussion will focus on our Yeast products, but. Adv. Yeast
  • 6. Page CONFIDENTIAL Confidential Why Should You Consider DSM BP&S We are the newcomer to the USA Ethanol Industry So we have to innovate and work harder to earn and keep your business! DSM DOES NOT charge Technology or Ferm Fees All In $/kg Pricing! DSM Yeast produces a Cleaner Ethanol! DSM Yeast delivers an average of 3% more ethanol compared to non GMO industry standard with the same or lower amounts of acetaldehyde & acetal & other fusels Glycerol reduction (up to 68%) Lower energy costs by increasing drying and evaporator efficiency Improves whole stillage separation efficiency & corn oil recovery We believe our nitrogen uptake pathway converts more carbon to useful amino acids & polypeptides thereby improving the quality of your DDGS We offer Monthly Lab Analysis, Quarterly Business Reviews & Regular Operator Training. We are MORE than a delivery & an invoice! 5
  • 7. Page CONFIDENTIAL Confidential DSM Yeast has very good acid tolerances showing up to 0.5% LA + 0.2% AA with negligible inhibition to finish DSM Yeast DO NOT contribute to the production of Acetic or Lactic acids eBoostTM GT offers high GA reductions (~50%) compared to traditional yeasts, and further increases shown over other GA expressing yeasts A recent mini-ferm trial showed additional 25% GA reduction eBOOST & eBOOST GT are produced in the U.S. / All regulatory approvals in place (MCAN*, GRAS) At this time we offer only Active Dry Yeast (ADY) 6 I can share some Performance Data but first Lets talk about what is important to you *Microbial Commercial Activity Notices (MCANs) Why Should You Consider DSM BP&S
  • 8. Page CONFIDENTIAL Confidential 7 Lets focus on your interest What is most important to you when choosing a yeast supplier? What are your expectations from a yeast supplier? What do you wish a yeast supplier would offer or innovate? Other thoughts/questions/coaching for us? Are you making or considering making hand sanitizer? Do you have an ability to test our yeast in a mini ferm or lab trial? If so, do you place much value is such tests? Is there an appetite now to trial one or both of our yeast? Have you had challenges optimizing your plant to a new yeast in the past? If so, what expectations do you have for DSM in a potential trial or switch? We are offering very low cost trials at this time!
  • 9. Page CONFIDENTIAL Confidential DSM eBOOST yields CLEAN Ethanol Lab Test Measurements in Fermentation Broth 8 Mash Yeast Acetaldehyde (mg/l) % of ER Corn Mash #1 Ethanol Red 77.5 100 Corn Mash #1 eBOOST 65.9 85 Corn Mash #2 Ethanol Red 101 100 Corn Mash #2 eBOOST 73.6 73 Mash Yeast (Acetal) 1,1-dieth oxyethane (mg/l) % of ER Corn Mash #1 Ethanol Red 6.77 100 Corn Mash #1 eBOOST 7.56 112 Corn Mash #2 Ethanol Red 10.9 100 Corn Mash #2 eBOOST 8.21 75 Temperature Profile for this testing was SOF to 22 Hrs: @ 94 F 22 Hrs to 36 Hrs @ 92.5 F 36 Hrs to 69 Hrs (EOF) @ 89.6 F Measurements were done from fermentation drop samples Drop samples were centrifuged and supernatants collected and frozen Samples sent to a certified third-party analytical lab for acetaldehyde and 1,1-Diethoxyethane (acetal) analysis by GC Lets talk Commercial Results
  • 10. Page CONFIDENTIAL Confidential Commercial trial results with Same corn, more value eBOOST compared to market standard: About 3% avgerage EtOH yield improvement Down to 68% glycerol reduction 32% 45% 38% 50% 40% 41% 38% 41% 0% 20% 40% 60% 80% 100% Market standard Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average Low glycerol levels eBOOST 2.0% 5.0% 4.7% 3.5% 2.1% 1.9% 2.5% 3.1% 98% 100% 102% 104% 106% Market standard Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average EtOH yield improvement eBOOST Equal or very similar performance observed with eBOOST GT In todays low price market your plant could make the same ethanol with less corn input cost! How does DSM compare with other Market Leaders?
  • 11. Page CONFIDENTIAL Confidential Page 10 Outperforms competitors Best yield potential in the market 2.5 - 5% 50 - 60% Equal or very similar performance observed with eBOOST GT Let me share details on in Plant Performance Data for eBoost GT Vs a Competitor Advanced Yeast
  • 12. Page CONFIDENTIAL Confidential Charts at Fermentation drop Trial-O-Rama eBOOST delivers higher EtOH and EtOH/Liq solids at drop 11 息 2019 DSM. All rights reserved. eBOOST delivers consistently higher EtOH titers and yields at drop vs. the other two strains. Compt 1 - Baseline Compt 2 eBOOST Compt 2 eBOOST Ethanol Ethanol/Liq solids Compt 1 Re-baseline Compt 1 - Baseline Compt 1 Re-baseline
  • 13. Page CONFIDENTIAL Confidential eBOOST GT Recipe Key Operating Conditions 12 Propagator Liq solids ~ 20%; Yeast Pitch 1 g/l with opportunity for optimization. Urea ~ 1 g/l No GA Propagation time of ~ 8-9 hrs Temperature: 90-93 F Main Fermenter Mash solids of 32-34% Urea ~1.0- 0.8 g/l 50% of normal GA dosage Plant standard temperature staging 92 88 F targeted ranges; In sustained commercial use, eBOOST GT had good finish with high ethanol titers even when fermentation temperatures occasionally peaked at 95F First, let me share some sugar consumption data vs a competitor using this recipe
  • 14. Page CONFIDENTIAL Confidential 13 eBOOST GT vs. Comp Yeast - Sugar Profiles @ Recent Ferm Trial Equal or very similar performance observed with eBOOST
  • 15. Page CONFIDENTIAL Confidential 14 Evaluation of performance eBOOST GT vs. Comp Yeast @ Recent Ferm Trial This data is @ a 50% reduction in GA which can be optimized up to 70%. eBOOST GT has a higher tolerance for glucose peak compared to competitor yeast eBOOST GT produces less Glycerol during fermentation No significant difference in lactic and acetic acid production compared to current plant levels. Equal or very similar performance observed with eBOOST

Editor's Notes

  • #4: Biofuels included in