Experience with Results
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Kristy Wendt has extensive experience characterizing materials using various microscopy techniques. She has worked with clients from Washington University to analyze samples ranging from nanoparticles to biological tissues. Wendt enjoys developing new processing protocols to image challenging samples. She also trains others and collaborates across facilities to further characterization capabilities.
Experience with Results
- 1. Kristy D. Wendt Material Surface Characterization: Partnership Building and Characterization Experience
- 2. Nano Research Facility Core Laboratories FEI NovaTM 230 FE Fabrication Laboratory NanoSEM (EDS) Elemental Mapping Particle Technology Laboratory FEI TecnaiTM Spirit TEM Leica UC7 Ultramicrotome Surface Characterization Laboratory Veeco NanomanTM AFM Bio-Imaging Laboratory Zeiss LSM 710 Confocal Microscope
- 3. Starting On Campus: Training Protocol Development EM Core Facility Collaboration
- 4. Image and Technique Portfolio: Image Processing FEI Tecnai SpiritTM Quantifying Size Dispersion TEM of ~200 nm Ag Nanocubes Binary Image with Global Threshold Client: Dr. Younan Xia, Washington University Sample: ”Bottom up” shape controlled synthesis silver and gold nanoparticles Expectations: Quantification of size dispersion 500 nm 500 nm
- 5. Image and Technique Portfolio: FEI NovaNanoTM SEM Finding the Right Angle Client: Dr. Brent Riggs, NRF Sample: Photolithography, Fabrication Expectations: Measure the thickness, density, and composition of films, identify profile shape, identify contamination and trace impurities
- 6. Image and Technique Portfolio: FEI NovaNanoTM SEM Troubleshooting Difficult Samples Client: Dr. Pratim Biswas, Washington University Sample: TiO2 crystal films Expectations: Difficult to image sample, high -contrast/charging Solution: Sputter coating current, reduced voltage, immersion mode, increased WD
- 7. Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Surface Characterization Client: Dr. Akira Ohnuma, Washington University Sample: Asymmetric hybrid colloidal particles by precipitation polymerization Expectations: Characterize a surface modified polystyrene bead
- 8. Image and Technique Portfolio: FEI NovaNanoTM SEM Murine Kidney Glomerulus Client: Dr. Jeff Miner, Washington University Sample: Kidney organogenesis in murine models Expectations: Processing protocol development, basement membrane preservation
- 9. Image and Technique Portfolio: FEI NovaNanoTM SEM Aluminum Polarization Filters Client: Dr. Victor Gruev, Washington University Representative Research Area: Nanofabrication of aluminum polarization filters Expectations: Side wall analysis
- 10. Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Instrument Limit Client: Dr. Byungkwon Lim, Washington University Sample: Palladium-platinum bimetallic nanocrystals Expectations: Ultrahigh magnification, show dendritic morphology; enhanced activity toward oxygen reduction in the fuel cell industry.
- 11. Image and Technique Portfolio: Efficient Sessions for Large Group Projects Client: Dr. Kathy Hafer, Washington University Sample: lambda phage Expectations: Provide large scale learning experiment, including processing and imaging for lambda phage
- 12. Image and Technique Portfolio: Nanotoxicity in Yeast Client: Dr. Yujie Xiong, Mark Brunson (REU) Washington University Sample: Saccharomyces cerevisiae Expectations: Processing protocol development for saccharomyces cerevisiae
- 13. Image and Technique Portfolio: FEI NovaNanoTM SEM Silica Chips on Silicon Substrate Client: Dr. Lan Yang, Lina He Washington University Sample: Silica microresinators on silicon substrates Expectations: Lower contrast image on difficult sample, ETD, longer working distance
- 14. Image and Technique Portfolio: Qualitative Analysis by EDS Elemental Mapping/Overlay Secondary Electrons Platinum 1 µm Overlay Tin balls Overlay
- 15. Image and Technique Portfolio: Line Mapping Ag and Pt Nanoparticles Silver Nanoparticles on Carbon Platinum Gold on Carbon
- 16. Image and Technique Portfolio: Nanoskiving Conventional Top Down Approach High capital Operating costs No non-planar surfaces Useful Material class restrictions Versatile Highly Developed
- 17. Conventional to Unconventional Techniques: Conventional Processing of Biological Tissue Fixation High Contrast Dry Thin -Osmium tetroxide -Ethanol Gradient -Resin Infiltration -Lead Citrate -Thermal Polymerization -Uranyl Acetate -Section 65 nm
- 18. Image and Technique Portfolio: Nanoskiving PDMS Cast and cure epoxy Ultramicrotomy EPOXY Thin-section ~70nm PDMS Separate epoxy EPOXY Deposit metal EPOXY Embed in epoxy and cure EPOXY EPOXY Knife blade
- 19. Image and Technique Portfolio: Nanoskiving PDMS Epoxy Au on epoxy Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577 Epoxy Knife
- 20. Image and Technique Portfolio: Nanoskiving 1. Nanostructure manipulation 2. 3D Stacking 3. No clean room Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577
- 21. Collaborating with Nanoscale Informal Science Education Network Participating in WU’s Educational Research Group Organizing and Hosting Public Outreach Festivals at WU
- 22. Developing Outreach: Nanodays at the Saint Louis Science Museum
- 23. Managing Research Experience for Undergraduates (REU) Program
- 24. Kristy D. Wendt -Broad range of material and biological EM processing -Enjoys addressing unique challenges -Novel protocol development -Fabrication and particle analysis -Training and partnership building -Works well with a broad range of clients to get beautiful results