Friday Seminar 15 10 2004
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The document summarizes the ADF MAGE-ML Tool, which facilitates the standardization and exchange of array design data. The tool can convert Array Design File (ADF) formats to MAGE-ML and validate files against standards. It supports various microarray applications and file formats. The validation process checks file structures, controlled vocabularies, and ontologies to ensure data quality.
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Ad
Friday Seminar 15 10 2004
- 1. Facilitating Standardization and Exchange of Array Design ADF MAGE-ML Tool Pierre Marguerite Friday Seminar EBI Microarray Informatics Team 15 October 2004
- 2. ADF MAGE-ML Tool Application stand-alone plateform independant Supports: Simple/Complex microarray layout Differents microarray applications gene_expression snp_detection comparative_genomic_hybridization binding_site_identification Others (minimal) Respects Good practices
- 4. MAGE-ML (MAGE-OM) Description Biosequence Array Array Design DesignElement DesignElement
- 7. Array Design File adh adr adc Header contacts Technical Information
- 8. Array Design File adr adc Reporters Features Feature /Reporter
- 9. Array Design File Composite Characteristics Map to reporters
- 10. ADF version differences 3 parts (files) instead of 1 As Workbook or text files No Reporter Identifier item No Reporter Group [role] item New Chromosome item New Chromosome_band item New Species item
- 11. 2 mandatory steps : Validation Conversion
- 12. Validation File format validation: File content validation Validation of controlled vocabulary MGED ontology terms Approved Databases (Tags, Accession numbers) Automatic curation (when possible)
- 13. Validation two levels of checking: Relaxed Strict two execution modes : A complete mode A step-by-step mode Error Log : for correction
- 14. Checking lists (header) File/Data structure checklist: Header file is a tab-delimited-file Item names are correct or can be identified if an item is not identified, it is skipped. All mandatory items are present in the header Data/file content checklist Correct field value format Possible value types: "Integer" "Free Text" "Controlled vocabulary" "MGED ontology term" "DatabaseEntry" "Sequence" "Species" Check single multiple value
- 15. Checking lists (feature reporter) Feature Reporter file File/Data structure checklist: Header File is correct (structure and data ) FeatureReporter file is a tab-delimited-file Header item names are correct (unknown items are skipped) All mandatory items are present. item cardinalities and dependences are correct. Database tags are approved and database accession numbers are correct Item order is correct (Optional, do not fail the checking) Field dependences are correct Data/file content checklist FeatureReporter file structure must be correct Mandatory Field are present. Field cardinalities and field value multiplicities must be correct. Field values are in a mandatory format Database tags are approved by ArrayExpress and are supplied in lower caseand between square brackets Database ID are correct Ontology terms are correct (MGED ontology) Sequences are correct following the associated polymer type (DNA, RNA, protein): Integer field values are correct Duplicate features must not exist Duplicate Reporter (equal names) must have the characteristics.
- 16. Checking lists (composite) CompositeSequence File/Data structure checklist: Feature Reporter file must be correct (structure and data) CompositeSequence file is a tab-delimited-file Header item names are correct. (Unknown items are skipped) All mandatory items are present. Header item cardinalities and dependences are correct Column order is correct (non mandatory) Data/file content checklist Composite file structure must be correct All mandatory fields are present. Field cardinalities are correct Field values are in expected format. Field multiplicity is correct (same as Feature/Reporter) Names in map are reporter or composite sequence names No duplicate CompositeSequences (same names)
- 17. Checking lists Header item names are correct All mandatory items are present All mandatory fields are present. No Duplicate features Duplicate Reporter (equal names) must have the characteristics. No duplicate CompositeSequences (same names) Names in map are reporter or composite sequence names
- 18. 油
- 19. 油
- 20. MGED Ontology / DAML+OIL
- 22. User modes
- 23. Implementation - technical choices -MAGE-stk JaxB Configuration (default parameters) Performance: 4000 features : ~10 minutes
- 24. Installer - izpack http://www.izforge.com/izpack/
- 26. 油
Editor's Notes
- #3: Vocabulaire contr担l辿
- #5: 16 packages au total 6 pour design
- #8: Redondance des informations Plus claire et plus lisible Facilement cr辿able (tableur)
- #9: Facile a lire et a comprenddre cr辿er ->redondance dinformation
- #10: Facile a lire et a comprenddre cr辿er ->redondance dinformation
- #14: Relaxed : allowing the usual mistakes (if they can be identified as well). Strict : file must exactly match the specification. A complete mode , which checks whole data; In that case, the process will not stop if an error is identified. A step-by-step mode : once an error is found, the process will stop, allowing a correction of errors one by one (for small data set or known small error numbers);