Genetic engineering Course Overview
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This document provides an overview and course requirements for a biotechnology class taught by Mr. Veerendra Singh Nagoria. Students must attend at least 75% of classes to be eligible to sit for the final exam. Assignments have defined deadlines and participation during lectures will contribute to the student's grade. The course will cover fundamental biotechnology concepts and techniques including DNA replication, transcription, translation, restriction enzymes, cloning, transformation of host cells, screening of recombinant clones, and applications of genetic engineering. Students will be continuously evaluated through assignments, presentations, and participation with a final exam accounting for 70% of the overall grade.
Genetic engineering Course Overview
- 1. Course Overview Mr. Veerendra Singh Nagoria Assistant Professor Biotechnology, School of Life Science, Rai University Ahmedabad Veerendra.nagoria@gmail.com, veerendra@outlook.com
- 2. Minimum Attendance requirement to sit for final semester examination is 75% No Assignment after defined time limit Open to discussion on Web over Google group. PPTs can be taken from my 際際滷share account but it is limited in nature consult books Participation during lecture session will be having additional weightage
- 3. CONTINUOUS EVALUATION 30 Marks Mid-Term Assessment As per examination Assignment 1 10 Marks Viva on Assignment 1 5 Marks Assignment 2 10 Marks Viva on Assignment 2 5 Marks Class Presentation 15 Marks Class Participation 5 Marks END SEMESTER EVALUATION 70 Marks
- 4. Introduction to concepts, Definitions, Discoveries Overview of fundamental processes: Replication, Transcription, Translation Basic Methodology Restriction modification system and Restriction Enzymes DNA modification enzymes: Nucleases, Polymerases, Enzymes modifying the ends of DNA molecule
- 5. Vectors: Plasmids, Bacteriophages, Cosmid, Phagemid, Artificial chromosomes Host cell types- prokaryotic and eukaryotic Transfer of vector into host cell: Transformation, Transfection, Packaging of phage DNA, Alternative DNA delivery methods
- 6. Cloning from mRNA, Genomic DNA Cloning after restriction digestion; blunt and cohesive Creation of cloning site by PCR Cloning using Linkers and Adapters Cloning by recombination; GATEWAY technology TA Cloning, TOPO Cloning, Ligation free cloning
- 7. Screening methods; Chromogenic substance, Insertional inactivation, Complementation of defined mutation Selecting recombinant clones; Reporter gene, elimination of non-transformed cells, identification of clones having Recombinant DNA Sequence Dependent screening; Colony hybridization, Chromosome walking, Screening by PCR, Gene Tagging
- 8. Genetic Engineering & Biotechnology; protein production, protein engineering, scale up Medicinal & Forensic Application of Gene Manipulation; Diagnosis, Treatment and DNA profiling