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Haemophilus influenzae
                                    MF II 12.grupa L.朝rone
                                                 RSU, 2012




H. influenzae, in a Gram stain of a sputum sample
   Gr- n笛jia (coccobacillus) ar polisahar朝du kapsulu
   Obligts cilvka paraz朝ts (tikai cilvka)
   Prnese caur respiratoro ce勅u
   Nekust朝gas, neform sporas, aerobas
   Tipiska Gr- 邸笛nas sienas strukt笛ra: LPS ar
    endotoks朝na aktivitti; rj membrna satur
    specifiskus prote朝nus (tiem ir noz朝me
    epidemiolo庁iskaj izmekl邸an)
Patogenittes faktori
 Polisahar朝du kapsulas, kas veidota no
  poliribitola ribozes fosfta PRP, antigenitte
  nosaka 6 serolo庁iskos tipus: a,b,c,d,e,f.
 Tie邸i b tips izraisa smagas,invaz朝vas slim朝bas-
  mening朝tu, septisku artr朝tu, epiglot朝tu.
 Neiekapsults un l朝dz ar to 束untypeable損
  kolonijas ar朝 izraisa slim朝bas, 朝pa邸i
  sinus朝tus, vidusauss iekaisumus, bet parasti
  neinvaz朝vus.
Patogenittes faktori
 Haemophilus influenzae type b (Hib) produc
  pilus, kas regul piesaisti pie cilvka 邸笛nm
  veidotas no pil朝na jeb HifA. Hib piliem nav
  neviens papildkomponents, sastv tikai no
  pil朝na.
 IgA proteze- noz朝m朝gs faktors nontypeable
  H.influenzae, kas iek勅auta normlaj flor.
pato庁enze
 Aug邸jie elpce勅i tiek kolonizti
 IgA proteze degrad sekretto IgA, dodot
  iespju piek勅auties elpce勅u mukozai
 Kad iesakojies mukoz, var tikt asin朝s-
  baktermija (ar piliem piesaists pie
  eritroc朝tiem, endokard朝ts)
 Izplats l朝dz smadzeu apvalkiem- mening朝ts.
  95% to izraisa b kapsula. Nekapsultie prsvar
  lokli, respiratorm slim朝bm.
 Ttad, pato庁enz iesaist朝ta antifagoc朝tiska
  kapsula un endotoks朝ns.
Kl朝nika
  Iekapsultie:                                 Neiekapsultie:
  1. Mening朝ts. Brniem no                       Vidusauss iekaisums
      3-36 mne邸iem.                             Pneimonija
  2. Ak笛ts epiglot朝ts.                           Sinus朝ts
  3. Septisks artr朝ts                            HOPS saasinjums
      (brniem)
  4. Sepse (sevi邸跳ie,ja
      defekt朝va liesa)
  5. pneimonija

Terapija: II,III paaudzes cefalospor朝ni; Hib vakc朝na (konjugts), pas朝v imunizcija- no
mtes caur placentu un pienu.
Diagnostikas algoritms
I Izmekljamais materils
- Cerebrospinlais likvors, sinovilais likvors, paraugi no respirators sistmas dzi勅km
vietm
II Transportbarotne
-lumblais punktts brauks ar 束OKOLDES損 barotni vai modifictas Tayer-Martin barotnes
-respiratorais materils ar Stjuarta u.c. Vienkr邸ajm barotnm, universlajm barotnm.
III kultiv邸ana
Aug邸anas barotn jb笛t aug邸anu-stimuljo邸iem faktoriem: hemin (x faktors (hma
sastvda勅a)) un NAD (V faktors 束vitamin損) . Aitas (vai kda cita) asinis r笛p朝gi kars 15 min
80属 C, lai likvidtu NAD inhibitorus un liztu eritroc朝tus,lai atbr朝votu heminu un NAD.
Izmanto 束邸okoldes損 agaru! Vislabk aug pie augstas CO2 konc. Un pie 37属C
IV Identifikcijas metodes
 NB! Nepiecie邸ami abi mintie faktori! Ja baktrija augs, tad ticami, ka t ir H.influenzae
     (H.parainfluenzae, piemram, nav nepiecie邸ami abi faktori, lai augtu)
 Definit朝vu identifikciju var veikt ar bio跳朝misku testu- QUELLUNG (kapsulrs
     piet笛k邸anas reakc.)
 K ar朝 ar Latex aglutincijas testu- nosaka kapsulas polisahar朝du
 Fluorescent antignu iez朝m邸ana (ELISA)
Ttad...


Vitli noz朝m朝gi ir ABI aug邸anas faktori!
                                                         Plakanas, pelc朝gi br笛nas
                                                      kolonijas, 1- 2 mm diametr
Paldies!

Haemophilus influenzae on Chocolate Horse Blood Agar

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Haemophilus influenzae

  • 1. Haemophilus influenzae MF II 12.grupa L.朝rone RSU, 2012 H. influenzae, in a Gram stain of a sputum sample
  • 2. Gr- n笛jia (coccobacillus) ar polisahar朝du kapsulu Obligts cilvka paraz朝ts (tikai cilvka) Prnese caur respiratoro ce勅u Nekust朝gas, neform sporas, aerobas Tipiska Gr- 邸笛nas sienas strukt笛ra: LPS ar endotoks朝na aktivitti; rj membrna satur specifiskus prote朝nus (tiem ir noz朝me epidemiolo庁iskaj izmekl邸an)
  • 3. Patogenittes faktori Polisahar朝du kapsulas, kas veidota no poliribitola ribozes fosfta PRP, antigenitte nosaka 6 serolo庁iskos tipus: a,b,c,d,e,f. Tie邸i b tips izraisa smagas,invaz朝vas slim朝bas- mening朝tu, septisku artr朝tu, epiglot朝tu. Neiekapsults un l朝dz ar to 束untypeable損 kolonijas ar朝 izraisa slim朝bas, 朝pa邸i sinus朝tus, vidusauss iekaisumus, bet parasti neinvaz朝vus.
  • 4. Patogenittes faktori Haemophilus influenzae type b (Hib) produc pilus, kas regul piesaisti pie cilvka 邸笛nm veidotas no pil朝na jeb HifA. Hib piliem nav neviens papildkomponents, sastv tikai no pil朝na. IgA proteze- noz朝m朝gs faktors nontypeable H.influenzae, kas iek勅auta normlaj flor.
  • 5. pato庁enze Aug邸jie elpce勅i tiek kolonizti IgA proteze degrad sekretto IgA, dodot iespju piek勅auties elpce勅u mukozai Kad iesakojies mukoz, var tikt asin朝s- baktermija (ar piliem piesaists pie eritroc朝tiem, endokard朝ts) Izplats l朝dz smadzeu apvalkiem- mening朝ts. 95% to izraisa b kapsula. Nekapsultie prsvar lokli, respiratorm slim朝bm. Ttad, pato庁enz iesaist朝ta antifagoc朝tiska kapsula un endotoks朝ns.
  • 6. Kl朝nika Iekapsultie: Neiekapsultie: 1. Mening朝ts. Brniem no Vidusauss iekaisums 3-36 mne邸iem. Pneimonija 2. Ak笛ts epiglot朝ts. Sinus朝ts 3. Septisks artr朝ts HOPS saasinjums (brniem) 4. Sepse (sevi邸跳ie,ja defekt朝va liesa) 5. pneimonija Terapija: II,III paaudzes cefalospor朝ni; Hib vakc朝na (konjugts), pas朝v imunizcija- no mtes caur placentu un pienu.
  • 7. Diagnostikas algoritms I Izmekljamais materils - Cerebrospinlais likvors, sinovilais likvors, paraugi no respirators sistmas dzi勅km vietm II Transportbarotne -lumblais punktts brauks ar 束OKOLDES損 barotni vai modifictas Tayer-Martin barotnes -respiratorais materils ar Stjuarta u.c. Vienkr邸ajm barotnm, universlajm barotnm. III kultiv邸ana Aug邸anas barotn jb笛t aug邸anu-stimuljo邸iem faktoriem: hemin (x faktors (hma sastvda勅a)) un NAD (V faktors 束vitamin損) . Aitas (vai kda cita) asinis r笛p朝gi kars 15 min 80属 C, lai likvidtu NAD inhibitorus un liztu eritroc朝tus,lai atbr朝votu heminu un NAD. Izmanto 束邸okoldes損 agaru! Vislabk aug pie augstas CO2 konc. Un pie 37属C IV Identifikcijas metodes NB! Nepiecie邸ami abi mintie faktori! Ja baktrija augs, tad ticami, ka t ir H.influenzae (H.parainfluenzae, piemram, nav nepiecie邸ami abi faktori, lai augtu) Definit朝vu identifikciju var veikt ar bio跳朝misku testu- QUELLUNG (kapsulrs piet笛k邸anas reakc.) K ar朝 ar Latex aglutincijas testu- nosaka kapsulas polisahar朝du Fluorescent antignu iez朝m邸ana (ELISA)
  • 8. Ttad... Vitli noz朝m朝gi ir ABI aug邸anas faktori! Plakanas, pelc朝gi br笛nas kolonijas, 1- 2 mm diametr
  • 9. Paldies! Haemophilus influenzae on Chocolate Horse Blood Agar

Editor's Notes

  • #2: Avots: http://en.wikipedia.org/wiki/Haemophilus_influenzae
  • #4: HOPS slimnieki bie転i inficjas ar no type H.i. Un tas paasina HOPS
  • #5: Although non-typeableHaemophilusinfluenzae (NTHi) are normally commensal flora of the upper respiratory tract, they can cause otitis media, sinusitis, pneumonia, chronic bronchitis, bacteremia, septic arthritis, and endocarditis. Invasive infection appears to arise when a strain evades normal host defenses. One factor that has been hypothesized as important to pathogenesis of such infection is an IgA protease produced by NTHi
  • #7: Haemophilusinfluenzae produces beta-lactamases, and it is also able to modify its penicillin-binding proteins, so it has gained resistance to the penicillin family of antibiotics. In severe cases, cefotaxime and ceftriaxone delivered directly into the bloodstream are the elected antibiotics, and, for the less severe cases, an association of ampicillin and sulbactam, cephalosporins of the second and third generation, or fluoroquinolones are preferred. (Fluoroquinolone-resistant Haemophilusinfluenzae has been observed.)[10]Macrolide antibiotics (e.g., clarithromycin) may be used in patients with a history of allergy to beta-lactam antibiotics.[citation needed] Macrolide resistance has also been observed.[11]
  • #8: The Quellung reaction is a biochemical reaction in which antibodies bind to the bacterial capsule of Streptococcus pneumoniae, Klebsiellapneumoniae, Neisseria meningitidis, Haemophilusinfluenzae [1], Salmonella, and group B Strep (mnemonic: Some Killers Have Nice Shiny Bodies). The antibody reaction allows these species to be visualized under a microscope. If the reaction is positive, the capsule becomes opaque and appears to enlarge.
  • #9: http://textbookofbacteriology.net/haemophilus_2.htmlhttp://en.wikipedia.org/wiki/Haemophilus_influenzaehttp://www.sciencephoto.com/media/283309/enlarge
  • #10: file:///C:/Users/Linda_S/Desktop/Haemophilus%20influenzae%20on%20Chocolate%20Horse%20Blood%20Agar%20%20%20Flickr%20-%20Photo%20Sharing!.htm