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This document summarizes a seminar presentation about the evolutionary study of endosymbiosis between Hydra and Chlorella through comparative genomic analysis. The presentation compared genomic and transcriptomic differences between symbiotic and non-symbiotic Chlorella species and strains. It also discussed using RNA interference methods to knock down gene expression in Chlorella and observe the effects on symbiosis with Hydra. The goal is to understand how and why certain Hydra and Chlorella species can live in an endosymbiotic relationship while others cannot.
Hid 2
- 1. Evolutionary study of endosymbiosis between Hydra and Chlorella by comparative genomic analysis Ishikawa Masakazu DDA seminar 21, Feb. 2013
- 3. Why certain species can live endosymbiotically? Differences between symbiotic and non- symbiotic species
- 4. Hydra viridissima and Chlorella spp. ? Symbiosis with Clorella ? Both can live independently ? Easy to culture ? non-symbiotic species in same genus are known Kawaida et al.
- 6. Phylogenetic tree of Hydra H. vulgaris group H. oligactis group H. braueri group H. viridissima group Symbiotic ability with Clorella Red: Yes Black: No Kawaida et al., 2010
- 7. Phylogenetic tree of Chlorella Symbiotic ability with H. viridissima C. sorokiniana Red: Yes Black: No C. vulgaris sp. K10 sp. J8 sp. M8 C. lobophora
- 8. Kawaida et al., 2010
- 9. Kawaida et al., 2010
- 10. Comparative genomics SNPs Indels Chlorella sp. CGCAAATCGAAGGG C. vulgaris CGCATATC - - - GGG
- 12. Comparative transcriptomics Chlorella sp. C. vulgaris Expression level free-living free-living A B C D E A B C D E Genes Genes Chlorella sp. symbiotic Expression level
- 13. Knockdown by using RNAi method siRNA mRNA Expression of gene A is suppressed Expression level A B C D E Genes
- 14. Re-infection WT RNAi strain Aposymbiotic Hydra
- 15. ?How different between symbiotic and non-symbiotic states. ?How does Hydra regulate proliferation of Chlorella sp.