Segregation refers to the separation or isolation of a group based on characteristics such as race or class. The document tells a story about segregation on a farm, where horses ruled over cows and imposed harsh rules restricting the cows' access to areas and resources. Dissatisfied with this unequal treatment, the cows organized and proposed ending segregation by having a race where if the cow won, both groups would be treated equally. On the day of the race, the underdog cow unexpectedly defeated the horse, winning freedom and equal rights for the cows going forward.