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HIVHuman Immunodeficiency Virus
   Human Immunodeficiency VirusHIV is a retrovirus that uses its RNA and the hosts DNA to make viral DNA. It has a long incubation period.
HIV consists of a cylindrical center surrounded by a sphere-shaped lipid envelope. The center consists of two single strands of RNA.hiv virus
HIV LifecycleHost cells infected with HIV have a very short lifespan.
HIV continuously uses new host cells to replicate itself.
During the first 24 hours after exposure, the virus attacks or is captured by dendritic cells (type of phagocyte) in mucous membranes and skin.         HIV LifecyclePhases: binding and entry, reverse transcription, replication, budding, and maturationHIV (arrows) Infecting a T-lymphocyte
Viral ReplicationMethods of transmission:
Sexual transmission, presence of STD increases likelihood of transmission.
Exposure to infected blood or blood products.
Use of contaminated clotting factors by hemophiliacs.
Sharing contaminated needles (IV drug users).
Transplantation of infected tissues or organs.
Mother to fetus, perinatal transmission variable, dependent on viral load and mothers CD 4 count.Virus Transmission IntroductionHIV can be transmitted through:Blood-
Semen
Vaginal Secretion
Breast Milk"Body Fluids" that HIV cannot be transmitted through:Saliva Tears
Sweat
Feces
Urine    Transmission
Virus Transmission/PreventionActivities of HIV Transmission:Unprotected sexual contact

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Hiv

  • 2. Human Immunodeficiency VirusHIV is a retrovirus that uses its RNA and the hosts DNA to make viral DNA. It has a long incubation period.
  • 3. HIV consists of a cylindrical center surrounded by a sphere-shaped lipid envelope. The center consists of two single strands of RNA.hiv virus
  • 4. HIV LifecycleHost cells infected with HIV have a very short lifespan.
  • 5. HIV continuously uses new host cells to replicate itself.
  • 6. During the first 24 hours after exposure, the virus attacks or is captured by dendritic cells (type of phagocyte) in mucous membranes and skin. HIV LifecyclePhases: binding and entry, reverse transcription, replication, budding, and maturationHIV (arrows) Infecting a T-lymphocyte
  • 8. Sexual transmission, presence of STD increases likelihood of transmission.
  • 9. Exposure to infected blood or blood products.
  • 10. Use of contaminated clotting factors by hemophiliacs.
  • 11. Sharing contaminated needles (IV drug users).
  • 12. Transplantation of infected tissues or organs.
  • 13. Mother to fetus, perinatal transmission variable, dependent on viral load and mothers CD 4 count.Virus Transmission IntroductionHIV can be transmitted through:Blood-
  • 14. Semen
  • 16. Breast Milk"Body Fluids" that HIV cannot be transmitted through:Saliva Tears
  • 17. Sweat
  • 18. Feces
  • 19. Urine Transmission
  • 20. Virus Transmission/PreventionActivities of HIV Transmission:Unprotected sexual contact
  • 22. Mother to babyPrimary HIV SyndromeMononucleosis-like, cold or flu-like symptoms may occur 6 to 12 weeks after infection.
  • 24. fever
  • 25. rash
  • 31. no symptoms may be present symptoms
  • 32. Laboratory Diagnosis of HIV InfectionMethods utilized to detect:
  • 36. Virus in cultureELISA TestingFirst serological test developed to detect HIV infection.Easy to perform.Easily adapted to batch testing.Highly sensitive and specific.Antibodies detected in ELISA include those directed against: p24, gp120, gp160 and gp41, detected first in infection and appear in most individuals
  • 37. ELISA TestingELISA tests useful for:Screening blood products.Diagnosing and monitoring patients.Determining prevalence of infection.Research investigations.
  • 38. ELISA TestingDifferent types of ELISA techniques used:
  • 41. sandwich ELISA Sandwich
  • 42. AVOIDSexual intercourse : HIV can infect the mucous membranes directly or when entered through cuts and sores during sexual intercourse. Unprotected Vaginal or Anal intercourse may result in receiving the virus. Oral sex : The risk of HIV transmission through the throat, gums or any other oral membranes has a lower risk then receiving it from the vaginal or anal area.Sharing injection needles: Injecting a needle and sharing it with another person can result to transmitting of the virus. Mother to Child: A woman who has HIV can pass the virus directly to her child before, or during birth, or through breast milk. Breast milk contains HIV, small amounts of breast milk doesn't effect adults, it can be transmitted to infants.
  • 43. Treatment For HIVThere is no cure for HIV yet. Medical professionals are working on a cure for it, but there are some treatments.Drug therapy for patients who are devoted to taking all off their medication and have A CD4 count lower than 350 mL, which shows their immune system is subdued. Pregnant women and people who have kidney or neurological problem related to HIV, probably need treatment regardless of their CD4 Count. It's important to take all of their medication or the virus will quickly become resistant to the drugs.Antiretroviral drugs (ARVs)
  • 44. THANK H YOUSubmitted by: DeepikatripathiBScBiotechnology