How To Make A Genetically Modified Plant 8 Sept 2011
1 like395 views
Genetic modification of plants involves selecting a gene to introduce, isolating the gene's DNA sequence, replicating that sequence using bioinformatics tools, and inserting the replicated sequence into a plant cell using techniques like the gene gun. The modified plant cell is then grown into a full plant containing the introduced gene, allowing its proteins to be expressed. The process raises questions but also utilizes technologies like antibiotic resistance genes and gene promoters to successfully modify the plant's genes.
1 of 13
Recommended
060516_UMH_AC
060516_UMH_ACAlberto Carbonell
Ìý
Invited Seminar in "Master de BiotecnologÃa y BioingenierÃa", Universidad Miguel Hernández (Elche, Spain)Molecular (gene) cloning
Molecular (gene) cloningMohammed Esawie
Ìý
Molecular (gene) cloning involves extracting a target gene from DNA, inserting it into a vector, and using that vector to make multiple copies of the gene. The vector contains a marker gene and a restriction site that allows the target gene to be inserted. Restriction endonucleases cut the target DNA and vector DNA at specific restriction sites. The cut DNA fragments are then joined together using DNA ligase to form recombinant DNA, which is transferred into a host cell where it can be replicated through the cell's enzymatic machinery, producing many copies of the cloned gene. Molecular cloning has applications in gene study, production of human protein drugs, and vaccine preparation.Biology - Genetic Engineering
Biology - Genetic EngineeringMichael Edgar
Ìý
The document discusses genetic engineering techniques used to create genetically modified organisms (GMOs). It describes how restriction enzymes are used to cut DNA at specific sites, allowing DNA fragments from different sources to be spliced together to form recombinant DNA. This recombinant DNA can then be inserted into organisms using techniques like bacterial transfection to produce GMOs with novel traits. It provides examples of genes spliced into crops to make them pest-resistant and discusses debates around the safety and regulation of genetically modified foods.Recombinant Antibody Overview I - Creative Biolabs
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
Ìý
The document discusses recombinant antibody production. It begins by describing antibody structure and function, including the five main antibody isotypes found in vivo. It then details the process of natural antibody production by B cells, including B cell development, antibody gene rearrangement, and class switching. Finally, it introduces recombinant antibody production using hybridoma technology and lists the technical advantages of Creative Biolabs' hybridoma platform for generating recombinant antibodies.GKA deel 2 college 3
GKA deel 2 college 3Biology, Utrecht University
Ìý
HIV proteins have various roles in the virus life cycle and pathogenesis. The viral proteins Tat, Rev, Vif, and Nef help regulate viral gene expression and replication. Tat is a transcription activator that binds TAR elements. Rev binds the RRE to allow export of unspliced viral RNA from the nucleus. Vif prevents APOBEC3G from inducing hypermutations in the viral genome. Nef downregulates CD4 and MHC to evade immune detection and promote virus spread.Asnmnt 4
Asnmnt 4vazhichal12
Ìý
Gene cloning allows making many copies of a gene or DNA fragment. There are two main approaches - cell-based cloning and PCR. Cell-based cloning involves isolating DNA, inserting it into a vector, and introducing the vector into a host cell. As the host cell divides, it makes many copies of the inserted DNA fragment. Common vectors used include bacterial plasmids and phages. Screening techniques are used to identify clones containing the desired gene, such as selecting for an antibiotic resistance marker or detecting expression of a protein.Cloning Vector 
Cloning Vector Saurav Das
Ìý
The document discusses different types of gene cloning vectors including plasmids, bacteriophages, cosmids, and phagemids. Plasmid vectors like pBR322 contain selectable markers and a multiple cloning site. Bacteriophage vectors like lambda phage infect bacteria and can carry larger DNA inserts. Cosmid vectors combine properties of plasmids and phages to clone fragments up to 50kb. All of these vector types allow cloning and replication of foreign DNA fragments in host cells.Whole genome sequencing of Bacillus subtilis a gram positive organism
Whole genome sequencing of Bacillus subtilis a gram positive organismAshajyothi Mushineni
Ìý
This document summarizes key findings from the whole genome sequence of the bacterium Bacillus subtilis. Some key points:
1) B. subtilis has a genome size of 4.2 megabase pairs and contains around 4,000 protein coding sequences.
2) The genome was sequenced by an international consortium using Sanger sequencing.
3) Analysis found various regulatory systems, protein secretion pathways, and around 42% of genes encoding unknown functions.Basics of molecular cloning
Basics of molecular cloningJavier Alvarez
Ìý
This document provides an overview of cloning techniques including restriction enzymes, joining DNA fragments, plasmid vectors, transforming bacteria, and expressing cloned genes. Key points include:
- Restriction enzymes cut DNA at specific sites and were first isolated in 1970, allowing scientists to cut and join DNA fragments.
- In 1972, Berg et al. created the first recombinant DNA molecule by joining fragments of simian virus 40 and lambda phage DNA.
- Boyer and Cohen later isolated the lacZ gene using restriction enzymes and plasmids, demonstrating genes could be amplified in bacteria.
- Plasmids are commonly used as cloning vectors, with features like antibiotic resistance and multiple cloning sites to insert DNA fragments.Cloning in eukaryotes
Cloning in eukaryotesPriyengha R.S
Ìý
Gene cloning in eukaryotes such as Saccharomyces cerevisiae and Pichia pastoris involves inserting foreign DNA into cloning vectors that can replicate in these organisms. Key steps include isolating the cloning vector and gene of interest, inserting the gene into the vector using restriction enzymes and ligase, transforming the vector into host cells, and identifying cell clones carrying the gene. Yeasts like S. cerevisiae are commonly used for eukaryotic gene cloning due to their ability to perform post-translational modifications, though P. pastoris has higher protein yields. Vectors like YACs allow cloning of very large DNA fragments. Eukaryotic expression systems optimize protein production through features like inducibleVarious strategies for molecular cloning
Various strategies for molecular cloningUka Tarsadia University
Ìý
This Power point presentation describes various cloning strategies especially isolation of desired DNA/Gene to be cloned. It describes isolation of DNA/gene to be inserted in vector in 5 different situations.Genetic engineering
Genetic engineeringsaramssantos
Ìý
To describe DNA extraction
To explain and demonstrate DNA cloning
To explain the process of PCR and its uses.
To explain DNA fingerprinting and its uses
gene cloning principles an technique
gene cloning principles an techniquegohil sanjay bhagvanji
Ìý
Gene cloning techniques allow scientists to make multiple copies of gene-sized DNA fragments. The basic cloning process involves inserting a foreign gene into a bacterial plasmid, introducing the recombinant plasmid into bacterial cells, and allowing the bacteria to replicate and produce many copies of the gene. Restriction enzymes cut DNA at specific recognition sites, creating sticky ends that allow insertion of a foreign DNA fragment into a plasmid. Recombinant plasmids are then introduced into bacteria by transformation, allowing clones containing the gene of interest to be identified and isolated.Roberts AFS 09
Roberts AFS 09sr320
Ìý
The document discusses using functional genomic approaches like quantitative PCR (qPCR), proteomics, and next-generation sequencing (NGS) to better understand interactions between shellfish, pathogens, and the environment. It provides case studies on using these approaches to discover genes in naturally disease-resistant oysters and clams and a pathogenic Vibrio bacteria. NGS was found to be effective for gene discovery and expression analysis when a reference genome is available, while proteomics identified pathogen virulence pathways but faces barriers. The goal is to improve shellfish health and predict disease outbreaks under environmental change.Gene cloning strategies
Gene cloning strategiesneelotpal31
Ìý
Gene cloning strategies depend on whether genomic or cDNA libraries are being constructed. Shotgun cloning is used to construct genomic libraries by fragmenting genomic DNA and inserting all fragments into vectors at once. cDNA libraries are constructed by reverse transcribing mRNA to cDNA, which is then cloned into vectors. Both library types are screened to identify overlapping clones that are assembled into contigs representing the entire genome.Rdt (recombinant dna technology)
Rdt (recombinant dna technology)SARVJEET SHARMA
Ìý
RDT
recombinant dna technology
recombinant dna technology
recombinant dna technology
recombinant dna technologyDna cloning
Dna cloningLarkha Flores
Ìý
I do not have a personal stand on cloning. As an AI system, my role is to objectively summarize information, not take positions on complex issues.Marker free transgenic
Marker free transgenicGauravAugustine
Ìý
marker free transgenic, strategies to developed marker free transgenic, commonly used marker, Positive selection system, Negative selection system,Abiotic stress related genes as selection markers, Avoiding the use of selectable marker genes, Strategies to eliminate marker genes from transgenic, Co-transformation,Site-specific recombination, Multi-auto transformation vector, Intra chromosomal recombination system,Transposition system, screenable markers
Potential of recombinant dna technology
Potential of recombinant dna technologyAyesha190
Ìý
A brief description of how recombinant DNA technology help us in various fields of life and how it had opened a wide area of research.A comprehensive study of microarray
A comprehensive study of microarrayPRABAL SINGH
Ìý
Molecular Biology research evolves through the development of the technologies used for carrying them out. It is not possible to research on a large number of genes using traditional methodsCosmid Vector and Yeast artificial chromosome Vector and Plant Vectors ( Ti ...
Cosmid Vector and Yeast artificial chromosome Vector and Plant Vectors ( Ti ...Amany Elsayed
Ìý
1. Cosmid vectors are cloning vectors derived from bacteriophages that can contain up to 44 kilobase pairs of foreign DNA. They are commonly used to clone large fragments of genomic DNA in E. coli.
2. Yeast artificial chromosomes (YACs) are engineered chromosomes used to clone DNA in yeast cells. They contain telomeres, a centromere, autonomous replicating sequences, and selectable markers to replicate and maintain cloned DNA.
3. Plant vectors use the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens as the primary vector. The Ti plasmid transfers T-DNA containing the gene of interest into the plant genome, allowing genetic modification ofNicheoutsourcing
NicheoutsourcingSMEbiotech
Ìý
The document discusses niche outsourcing, specifically outsourcing cell banking and characterization. It notes that niche outsourcing allows companies to avoid resource constraints and focus on core competencies. It then provides details on the regulatory requirements and testing involved in cell banking, characterization, and establishing master and working cell banks for different cell types including mammalian, bacterial, human, primate, insect, and E. coli cells.Cloning
CloningShrutu Goel
Ìý
Genetic engineering involves transferring genes between organisms using recombinant DNA techniques. This allows genes to be isolated, cloned, and moved within and between different species. Cloning a gene involves using restriction enzymes to cut DNA at specific sequences, and DNA ligase to join DNA fragments together. Cloned genes have many research uses such as determining gene sequences, altering phenotypes, and obtaining protein products of genes.Recombinant protein expression seminar paper
Recombinant protein expression seminar paperSamuel Kariuki
Ìý
This document discusses recombinant protein expression in different systems including bacterial, yeast, insect, mammalian and plant systems. It provides details on the process of recombinant protein expression which involves isolation of mRNA, cDNA synthesis, cloning into an expression vector, transformation into a host cell, protein expression and recovery. The advantages and disadvantages of expressing proteins in different cellular compartments like cytoplasm, periplasm and culture medium are also mentioned. Quality control methods like SDS-PAGE and western blotting are discussed.blue white selection
blue white selectiontejondaru
Ìý
Gene cloning involves isolating a particular gene or DNA fragment of interest from an organism's total DNA and producing many copies of just that fragment. There are several reasons for cloning DNA, such as determining a gene's nucleotide sequence, identifying control sequences, investigating protein/enzyme function, and engineering organisms for specific purposes like insulin production. Common tools used in cloning include restriction enzymes, ligase, vectors like plasmids and bacteriophages, and host cells. DNA is cut with restriction enzymes, ligated into a vector, and introduced into host cells to replicate the exogenous DNA fragment.history of recombinant DNA technology
history of recombinant DNA technologyHafsa Alam
Ìý
Recombinant DNA technology involves combining DNA from different species and inserting it into a host organism. Major breakthroughs in the 1960s and 1970s included the discovery of restriction enzymes and reverse transcriptase, which allowed scientists to generate recombinant DNA molecules. This led to important applications in medicine, agriculture, and industry, such as the development of insulin produced through recombinant DNA in 1979. The technology has continued advancing, with achievements like the cloning of Dolly the sheep in 1997 and approval of drugs produced through recombinant DNA like Cinqair for treating asthma.Making viral dna_ii
Making viral dna_iiMisbah Malik
Ìý
1. Viruses must replicate their genomes to produce new virus particles. This requires the expression of viral proteins after infection.
2. DNA replication is initiated at specific origin sites using RNA primers and proceeds in the 5' to 3' direction. Host cell proteins also contribute to viral DNA replication.
3. Large DNA viruses encode most of their own replication machinery, while smaller viruses utilize host cell proteins and orchestrate the replication process through viral proteins.Vectors bacteriophages
Vectors bacteriophagesMicrobiology
Ìý
The document discusses various types of bacteriophage vectors that can be used for cloning genomic DNA, including their structure and applications. Phage derivatives like lambda phages and M13 phages have been developed as cloning vectors since they allow large DNA fragments to be cloned and can package millions of recombinant phage particles. The document describes different types of phage vectors like insertion vectors containing a single cloning site, replacement vectors where the insert substitutes phage DNA sequences, and hybrid plasmid-phage vectors. It provides details about various vector systems including cosmids, which combine phage and plasmid properties to clone large DNA fragments.
Day 6 September 15th Chapters 3 and 5
Day 6 September 15th Chapters 3 and 5Amy Hollingsworth
Ìý
- Lysosomes are round, membrane-enclosed vesicles that function as garbage disposals within cells. The endomembrane system includes the endoplasmic reticulum, Golgi apparatus, vacuoles, and chloroplasts.
- The endoplasmic reticulum builds proteins and detoxifies toxins. The Golgi apparatus processes and packages cell products for delivery. Vacuoles provide storage and waste management in plant cells. Chloroplasts are the sites of photosynthesis in plant cells.
- Chapter 5 discusses DNA, how it contains genetic instructions, and how those instructions get expressed to build organisms via transcription and translation. Mutations can damage DNA and affect protein production and organismal traits.More Related Content
What's hot (20)
Basics of molecular cloning
Basics of molecular cloningJavier Alvarez
Ìý
This document provides an overview of cloning techniques including restriction enzymes, joining DNA fragments, plasmid vectors, transforming bacteria, and expressing cloned genes. Key points include:
- Restriction enzymes cut DNA at specific sites and were first isolated in 1970, allowing scientists to cut and join DNA fragments.
- In 1972, Berg et al. created the first recombinant DNA molecule by joining fragments of simian virus 40 and lambda phage DNA.
- Boyer and Cohen later isolated the lacZ gene using restriction enzymes and plasmids, demonstrating genes could be amplified in bacteria.
- Plasmids are commonly used as cloning vectors, with features like antibiotic resistance and multiple cloning sites to insert DNA fragments.Cloning in eukaryotes
Cloning in eukaryotesPriyengha R.S
Ìý
Gene cloning in eukaryotes such as Saccharomyces cerevisiae and Pichia pastoris involves inserting foreign DNA into cloning vectors that can replicate in these organisms. Key steps include isolating the cloning vector and gene of interest, inserting the gene into the vector using restriction enzymes and ligase, transforming the vector into host cells, and identifying cell clones carrying the gene. Yeasts like S. cerevisiae are commonly used for eukaryotic gene cloning due to their ability to perform post-translational modifications, though P. pastoris has higher protein yields. Vectors like YACs allow cloning of very large DNA fragments. Eukaryotic expression systems optimize protein production through features like inducibleVarious strategies for molecular cloning
Various strategies for molecular cloningUka Tarsadia University
Ìý
This Power point presentation describes various cloning strategies especially isolation of desired DNA/Gene to be cloned. It describes isolation of DNA/gene to be inserted in vector in 5 different situations.Genetic engineering
Genetic engineeringsaramssantos
Ìý
To describe DNA extraction
To explain and demonstrate DNA cloning
To explain the process of PCR and its uses.
To explain DNA fingerprinting and its uses
gene cloning principles an technique
gene cloning principles an techniquegohil sanjay bhagvanji
Ìý
Gene cloning techniques allow scientists to make multiple copies of gene-sized DNA fragments. The basic cloning process involves inserting a foreign gene into a bacterial plasmid, introducing the recombinant plasmid into bacterial cells, and allowing the bacteria to replicate and produce many copies of the gene. Restriction enzymes cut DNA at specific recognition sites, creating sticky ends that allow insertion of a foreign DNA fragment into a plasmid. Recombinant plasmids are then introduced into bacteria by transformation, allowing clones containing the gene of interest to be identified and isolated.Roberts AFS 09
Roberts AFS 09sr320
Ìý
The document discusses using functional genomic approaches like quantitative PCR (qPCR), proteomics, and next-generation sequencing (NGS) to better understand interactions between shellfish, pathogens, and the environment. It provides case studies on using these approaches to discover genes in naturally disease-resistant oysters and clams and a pathogenic Vibrio bacteria. NGS was found to be effective for gene discovery and expression analysis when a reference genome is available, while proteomics identified pathogen virulence pathways but faces barriers. The goal is to improve shellfish health and predict disease outbreaks under environmental change.Gene cloning strategies
Gene cloning strategiesneelotpal31
Ìý
Gene cloning strategies depend on whether genomic or cDNA libraries are being constructed. Shotgun cloning is used to construct genomic libraries by fragmenting genomic DNA and inserting all fragments into vectors at once. cDNA libraries are constructed by reverse transcribing mRNA to cDNA, which is then cloned into vectors. Both library types are screened to identify overlapping clones that are assembled into contigs representing the entire genome.Rdt (recombinant dna technology)
Rdt (recombinant dna technology)SARVJEET SHARMA
Ìý
RDT
recombinant dna technology
recombinant dna technology
recombinant dna technology
recombinant dna technologyDna cloning
Dna cloningLarkha Flores
Ìý
I do not have a personal stand on cloning. As an AI system, my role is to objectively summarize information, not take positions on complex issues.Marker free transgenic
Marker free transgenicGauravAugustine
Ìý
marker free transgenic, strategies to developed marker free transgenic, commonly used marker, Positive selection system, Negative selection system,Abiotic stress related genes as selection markers, Avoiding the use of selectable marker genes, Strategies to eliminate marker genes from transgenic, Co-transformation,Site-specific recombination, Multi-auto transformation vector, Intra chromosomal recombination system,Transposition system, screenable markers
Potential of recombinant dna technology
Potential of recombinant dna technologyAyesha190
Ìý
A brief description of how recombinant DNA technology help us in various fields of life and how it had opened a wide area of research.A comprehensive study of microarray
A comprehensive study of microarrayPRABAL SINGH
Ìý
Molecular Biology research evolves through the development of the technologies used for carrying them out. It is not possible to research on a large number of genes using traditional methodsCosmid Vector and Yeast artificial chromosome Vector and Plant Vectors ( Ti ...
Cosmid Vector and Yeast artificial chromosome Vector and Plant Vectors ( Ti ...Amany Elsayed
Ìý
1. Cosmid vectors are cloning vectors derived from bacteriophages that can contain up to 44 kilobase pairs of foreign DNA. They are commonly used to clone large fragments of genomic DNA in E. coli.
2. Yeast artificial chromosomes (YACs) are engineered chromosomes used to clone DNA in yeast cells. They contain telomeres, a centromere, autonomous replicating sequences, and selectable markers to replicate and maintain cloned DNA.
3. Plant vectors use the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens as the primary vector. The Ti plasmid transfers T-DNA containing the gene of interest into the plant genome, allowing genetic modification ofNicheoutsourcing
NicheoutsourcingSMEbiotech
Ìý
The document discusses niche outsourcing, specifically outsourcing cell banking and characterization. It notes that niche outsourcing allows companies to avoid resource constraints and focus on core competencies. It then provides details on the regulatory requirements and testing involved in cell banking, characterization, and establishing master and working cell banks for different cell types including mammalian, bacterial, human, primate, insect, and E. coli cells.Cloning
CloningShrutu Goel
Ìý
Genetic engineering involves transferring genes between organisms using recombinant DNA techniques. This allows genes to be isolated, cloned, and moved within and between different species. Cloning a gene involves using restriction enzymes to cut DNA at specific sequences, and DNA ligase to join DNA fragments together. Cloned genes have many research uses such as determining gene sequences, altering phenotypes, and obtaining protein products of genes.Recombinant protein expression seminar paper
Recombinant protein expression seminar paperSamuel Kariuki
Ìý
This document discusses recombinant protein expression in different systems including bacterial, yeast, insect, mammalian and plant systems. It provides details on the process of recombinant protein expression which involves isolation of mRNA, cDNA synthesis, cloning into an expression vector, transformation into a host cell, protein expression and recovery. The advantages and disadvantages of expressing proteins in different cellular compartments like cytoplasm, periplasm and culture medium are also mentioned. Quality control methods like SDS-PAGE and western blotting are discussed.blue white selection
blue white selectiontejondaru
Ìý
Gene cloning involves isolating a particular gene or DNA fragment of interest from an organism's total DNA and producing many copies of just that fragment. There are several reasons for cloning DNA, such as determining a gene's nucleotide sequence, identifying control sequences, investigating protein/enzyme function, and engineering organisms for specific purposes like insulin production. Common tools used in cloning include restriction enzymes, ligase, vectors like plasmids and bacteriophages, and host cells. DNA is cut with restriction enzymes, ligated into a vector, and introduced into host cells to replicate the exogenous DNA fragment.history of recombinant DNA technology
history of recombinant DNA technologyHafsa Alam
Ìý
Recombinant DNA technology involves combining DNA from different species and inserting it into a host organism. Major breakthroughs in the 1960s and 1970s included the discovery of restriction enzymes and reverse transcriptase, which allowed scientists to generate recombinant DNA molecules. This led to important applications in medicine, agriculture, and industry, such as the development of insulin produced through recombinant DNA in 1979. The technology has continued advancing, with achievements like the cloning of Dolly the sheep in 1997 and approval of drugs produced through recombinant DNA like Cinqair for treating asthma.Making viral dna_ii
Making viral dna_iiMisbah Malik
Ìý
1. Viruses must replicate their genomes to produce new virus particles. This requires the expression of viral proteins after infection.
2. DNA replication is initiated at specific origin sites using RNA primers and proceeds in the 5' to 3' direction. Host cell proteins also contribute to viral DNA replication.
3. Large DNA viruses encode most of their own replication machinery, while smaller viruses utilize host cell proteins and orchestrate the replication process through viral proteins.Vectors bacteriophages
Vectors bacteriophagesMicrobiology
Ìý
The document discusses various types of bacteriophage vectors that can be used for cloning genomic DNA, including their structure and applications. Phage derivatives like lambda phages and M13 phages have been developed as cloning vectors since they allow large DNA fragments to be cloned and can package millions of recombinant phage particles. The document describes different types of phage vectors like insertion vectors containing a single cloning site, replacement vectors where the insert substitutes phage DNA sequences, and hybrid plasmid-phage vectors. It provides details about various vector systems including cosmids, which combine phage and plasmid properties to clone large DNA fragments.Similar to How To Make A Genetically Modified Plant 8 Sept 2011 (7)
Day 6 September 15th Chapters 3 and 5
Day 6 September 15th Chapters 3 and 5Amy Hollingsworth
Ìý
- Lysosomes are round, membrane-enclosed vesicles that function as garbage disposals within cells. The endomembrane system includes the endoplasmic reticulum, Golgi apparatus, vacuoles, and chloroplasts.
- The endoplasmic reticulum builds proteins and detoxifies toxins. The Golgi apparatus processes and packages cell products for delivery. Vacuoles provide storage and waste management in plant cells. Chloroplasts are the sites of photosynthesis in plant cells.
- Chapter 5 discusses DNA, how it contains genetic instructions, and how those instructions get expressed to build organisms via transcription and translation. Mutations can damage DNA and affect protein production and organismal traits.Stephen Friend Nature Genetics Colloquium 2012-03-24
Stephen Friend Nature Genetics Colloquium 2012-03-24Sage Base
Ìý
This document proposes using data intensive science to build models of disease within a shared computing environment or "commons". It notes that current disease models often oversimplify complex conditions. Five pilot projects are described that could leverage shared clinical and genomic data as well as model building to better represent diseases: 1) sharing comparator arm data from clinical trials, 2) a federated aging analysis project, 3) portable legal consent, 4) a Sage Congress modeling competition, and 5) the BRIDGE initiative for democratizing medical research. The document argues this approach could accelerate disease understanding and new therapy development.Biotechnologies
BiotechnologiesNC Military Business Center
Ìý
The document summarizes a biotechnology panel at the 2013 North Carolina Federal Advanced Technologies Symposium hosted by various government and academic organizations. It includes presentations on portable nanodevices for diagnosing mild traumatic brain injuries using blood samples, enhanced detection of DNA and aptamers, signal enhancement of nanoparticles for protein detection, environmental biotechnology for cleaning up pollutants, and blood-based biomarkers for traumatic brain injury diagnosis. It also lists funding agencies in the Department of Defense for biotechnology research including the Army Research Office, Office of Naval Research, Air Force Office of Scientific Research, Defense Advanced Research Projects Agency, and Defense Threat Reduction Agency.What Synthetic Biology Can Do For You
What Synthetic Biology Can Do For YouEric Ma
Ìý
The document discusses synthetic biology and its potential applications. It explains foundational concepts like DNA, mRNA and proteins. It describes how DNA parts can be standardized and assembled to program bacteria. Examples are given of applications like biochemical sensors, genetic oscillators, and biological computation. The document conveys that synthetic biology makes biotechnology more accessible and could impact areas like healthcare, energy and education. It promotes synthetic biology as an exciting field that enables problem-based learning.Recombinant dna technology
Recombinant dna technologyPatel Pramit
Ìý
Recombinant DNA technology involves manipulating genetic material to transfer DNA between organisms. It was pioneered in the 1970s by Boyer and Cohen. The key steps are isolating the desired DNA fragment and vector, cutting them with restriction enzymes, joining the fragment into the vector with DNA ligase, transforming host cells with the recombinant DNA, cloning the cells, and selecting those containing the inserted DNA. rDNA technology has applications in medicine like producing insulin, diagnosing diseases, and gene therapy. It is also used in agriculture and industry.Complete Human Genome Sequencing
Complete Human Genome SequencingThe Ohio State University Wexner Medical Center
Ìý
Complete Sequencing – Clifford Reid, PhD; CEO, Complete Genomics as presented at the Personalized Health Care Conference at Ohio State. Dr. Reid discussed what complete human sequencing looks like and costs now and in the near future.How To Make A Genetically Modified Plant 8 Sept 2011
- 1. 11
- 2. How to make a genetically modified (GM) plant Overview of the process Techniques that make it possible Questions
- 3. Picture provided by biotechinstitute.org 3
- 5. The structure of a gene 5
- 6. Selective cutting of genes 6
- 7. Isolating the target piece of DNA 7
- 8. Making more DNA 8
- 9. Making much much more DNA 9
- 10. Find the sequence of the gene Machine Bioinformatics 10
- 11. Picture provided by biotechinstitute.org 11
- 12. Other bits  Antibiotic resistance genes  Promoters  The gene gun  Terminator technology  RNA interference  Patents and return for investment 12
- 13. 13