ºÝºÝߣ

ºÝºÝߣShare a Scribd company logo
Figure3.Chromesomedamageeffectofsecondarymetabolitesextractin
hepG2cells.BN:bi-nuleated,MNs:micronucleus,NPBs:nucleoplasmicbridges,
NBUDs:nulearbuds,CPBI:cytokinesis-blockproliferationindex.PC:positive
control,NC:negativecontrol,SM:secondarymetabolitesextract(heat-dry).
Figure1.MutagenecityofseconderymetabolitesextractfromC.nuda(heat-
dry).NC:negativecontrol(H2O),SC:solventcontrol(DMSO),PC:positivecontrol.
S9:supernatantfractionofratliver.homogenate.
Figure2.Cytotoxicityandestimatedacuteoraltoxicityofseconderay
metaboliteextractfromC.nuda.LD50wasestimatedfromIC50in3T3NRUassay
basedonapredictionmodel:logLD50(mg/kg)=0.372¡ÁlogIC50
Treated
withcyto-B
TreatedwithKClTreatedwith100%
methonalfor30min
Giemsa-Wright
staining
mountedslides
HepG2cells
Poly-L-lysine
coatedcover
glass
2x10cells/mL
37C
5%CO2
washwith
D-PBS
17h
solutionIIfor3s
solutionIIIfor3s
Microscope
dumpedtheculture
medium
countedthenumberofMNs,
NPBsandNBUDsin1000BN
1000x
Freezedy
crudeextract
or
11.3
1.96
39.2
5.47
0
10
20
30
40
50
Freeze-dryextractHeat-dryextract
IC50(mg/mL)
Balb/c3T3cells
HepG2cells
EstimatedLD50
(mg/kgbodyweight):
3,397¡À741,772¡À45
Balb/c3T3cells
2.7¡Á10cells/mL
HepG2cells
1.0¡Á105
cells/mL
HeatdryextractFreezedryextract
37?C,12h
+100¦ÌLbacteria
+50¦ÌLsample
+500¦ÌLS9(+S9test)or
+500¦ÌLbuffer(-S9test)
2mLtopagar
(0.5%agar+0.05
Mbiotin/histidine)
10mLbottom
minimalagarplates
withouthistidine
37?C,20min37?C,48h
1-2¡Á109
CFU/mL
CountcoloniesS.Typhimurium
mutationstrains
Vortex
TA100,TA1535TA98,TA1538TA1537
-C-G-C-G-C-G-C-G
frameshifts
-G-G-G-
base-pairsubstitution
-C-C-C
frameshifts
DNAtarget:
Reversionevent:
Bacteriastrains:
Fiveconcentrationsofextract:
50,500,1000,2500,5000¦Ìg/plate
Feeze-dryandheat-dry
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0
10
20
30
40
50
NC(0)PC(0.2)SM(0.75)SM(1.5)SM(3)SM(6)SM(12)
Celldivisonindex
DNAdamageincecesin1000BNcells
Sampleconcentration(mg/mL)
No.ofMNsNo.ofNPBsNo.ofNBUDsCBPI
1
10
100
1000
10000
TA98TA100TA1535TA1537TA1538
Revertantsnumber
Bacteriastrains
Withmetabolizing(+S9)
NCSCPC50500100025005000Concentration(¦Ìg/plate)
1
10
100
1000
10000
TA98TA100TA1535TA1537TA1538
Revertantsnumber
Bacteriastrains
Withoutmetabolizing(-S9)
NCSCPC50500100025005000Concentration(¦Ìg/plate)
C.nudastrainLA82
shakerfor21days
Secondary
metabolites
1.WhatmanNo.1
filtermembrane
Heatdry
crudeextract
Filteration
vaccumfreezedriedfor72h
ToxicityAssessmentofSecondaryMetabolitesExtractfromClitocybenudaasNaturalFoodAntimicrobials
MingyuQiao1
,TianRen1
,LeiZhang1
,JeanWeese1
,Jin-TongChen2
,Tung-ShiHuang1
1
DepartmentofPoultryScience,AuburnUniversity,Auburn,AL,USA,2
DivisionofPlantPathology,TaiwanAgriculturalResearchInstitute,Taichung,Taiwan
Clitocybenudaisanedible
mushroomdistributedinEurope,
NorthAmerica,Australia,and
Asia.Inrecentyears,several
studiesshowedthatbothfruit
bodyandsecondarymetabolites
extractsfromC.nudaexhibited
promisingantimicrobialactivities
.(1,2)Ourpreviousstudyshowed
thatthesecondarymetabolitesextractofC.nudahadgood
antimicrobialactivitiesagainstvariousfoodbornepathogens
suchasSalmonellaTyphimurium,EscherichiacoliO157:H7,
Listeriamonocytogenes,etc.(3)Thismushroomsecondary
metabolitesextractwasalsofoundtohaveseveraladvantages
suchashighwatersolubility,widepHrange,goodthermal
stabilityandlowcost.However,notoxicityinformationof
thisseconderymetabolitesextractwasavailable.Therefore,
studiesaboutthetoxicityofsecondarymetabolitesextract
fromC.nudaareneededforensuringthesafetytoapplyas
naturallyoccurredantimicrobialsinfoods.
2.Cytotoxicityandestimatedacuteoraltoxicity
1.Mutagenicity(genotoxicity)
MaterialsandMethods
1.C.nudasecondarymetabolitesextractpreperation
3.Cytotoxicitytest(NRUassay)
Conclusions
DiscussionResultsIntroduction
1.Seeded96-Wellplateswith
2.Preparedextractsamples
andcontrolsinnewplates
4.Treatedcellswithdyes,
incubatefor3h
5.Dumpedandrinsedplates
3.Treatedcellswithsamples,
6.Extracteddyesbyshaking
for20min
7.Readabsorbanceof
platesatO.D.550nm
Objectives
TheInteragencyCoordinatingCommitteeontheValidation
ofAlternativeMethods(ICCVAM)hasvalidatedthatthe3T3
NRUassayhasahighsensitivity(c.a.95%)whenemployed
incombinationwithapredictionmodeltodistinguish
potentiallytoxicversusnon-toxicsubstances."Ifestimated
acuteoraltoxicityLD50>2,000mg/kgbodyweight(rat),
additionalanimaltestisneeded".(5)
Inthisstudy,freeze-driedC.nudasecondarymetabolites
extracthastheestimatedacuteoralLD50>2,000mg/
kgbodyweight.Additionally,noincreaseoftoxictywas
observedincellswithhumanmetabolizationenzymes
(hepG2).Therefore,thesecondarymetabolitesextractfrom
C.nudacanbeconsideredasnon-acuteoraltoxicbasedon
resultsfromthisstudyandstate-of-artrecommendations.
committeehasconcludedthatacombinationuseofa
bacterialreversemutationtestandaninvitromammalian
cellmicronucleustestarereliablefordetectionofmost
potentialgenotoxicsubstances."Ifallendpointsareclearly
negativeinadequatelyconductedtests,thenitcanbe
concludedwithreasonablecertaintythatthesubstancehas
nogenotoxicity".(6)
Inthisstudy,bothofthetwoinvitrogenotoxicityassays
showedclearnegativeresults,thereforethissecondary
metabolitesextract(freeze-dryandheat-dry)canbe
consideredasnon-genotoxic.
C.nudasecondarymetabolitesextractissuitabletobe
humanasnewnaturalfoodantimicrobials,andnoindication
oftoxictywasobservedaftermetabolizing.
Heatprocessingisnotexpectedtoincreasethetoxicityof
secondarymetaboliteextractfromC.nuda.
1.Barrosetal.J.Agric.FoodChem.2008,56,3856.
2.Chenetal.EvidBasedComplementAlternatMed.
2013,
3.ZhengHou.Antibacterialactivitiesofsecondary
metabolitesfromClitocybenuda.MSthesis.2013.
OECD.SeriesonTestingandAssessmentNo.129.
2010.
5.ICCVAM.NIHPublicationNo.07-4579.2006.
6.EFSA.EFSAJournal.2011,9,2379.
References
Toassessthetoxicityofsecondarymetabolitesextractfrom
C.nudaasnewnaturalfoodantimicrobials?
Toinvestigatetheimparctofheatprocessingonthetoxicity
effectofsecondarymetabolitesextractfromC.nuda?
Getthisposteronline
Acknowledgment
ThisresearchwassponsoredbyGraduateStudentThesisResearchAwardfrom
GraduateSchoolofAuburnUniversityandgenerousdonorMs.AbigailMacGregor.
2.Mutagenecitytest(bacteriareversemutationtest)
3.Chromosomedamageeffect(genotoxicity)
4.Genotoxicitytest(invitromicronucleusassay)
Added
sample
Ad

Recommended

§à§Ý§ñ §ã§Ñ§Û§ä
§à§Ý§ñ §ã§Ñ§Û§ä
virtualtaganrog
?
Curriculum vitae
Rodrigo Olivares
?
What have you learnt about technologies from the process of constructing this...
What have you learnt about technologies from the process of constructing this...
samsamaden6
?
HOW I DID IT
HOW I DID IT
Sheree Fadil
?
EDS LETTER
EDS LETTER
Sheree Fadil
?
FINAL COUNTDOWN
FINAL COUNTDOWN
Sheree Fadil
?
§ã§Ñ§Û§ä §à.§ß.
§ã§Ñ§Û§ä §à.§ß.
virtualtaganrog
?
nomadic empire.pdfgreegxwegrhcwrthchthcerh
nomadic empire.pdfgreegxwegrhcwrthchthcerh
ParthChauhan522968
?
How to Maintain Garden for Growing Seeds.ppt
How to Maintain Garden for Growing Seeds.ppt
Adinath Trading Company
?
YZM Food Truck Catalog for those who are interested to start food truck. it h...
YZM Food Truck Catalog for those who are interested to start food truck. it h...
tahirrasheid
?
WEEK 1(cookery-subject orientation).pptx
WEEK 1(cookery-subject orientation).pptx
sharlene sacristia
?
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeffrey Kaliel
?
Title Lorem Ipsum.pptx math tasty coffee
Title Lorem Ipsum.pptx math tasty coffee
cdiem040
?
Basic forms of matter and energy Matter and energy as a resource Scientific l...
Basic forms of matter and energy Matter and energy as a resource Scientific l...
rakesh921395
?
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
Aman Bansal
?
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
taqyed
?
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Sarvesh Bose
?
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
taqyea
?
Lecture 5.2 Understanding Demographic Change .pptx
Lecture 5.2 Understanding Demographic Change .pptx
shofalbsb
?
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
Taqyea
?
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
naitikbansal61
?
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
pegjpope
?
Anemia Presentation, introduction, causes (1) (2).pptx
Anemia Presentation, introduction, causes (1) (2).pptx
z6znrhjj6h
?
South Korea Dairy Market Trends and Growth 2025 to 2034
South Korea Dairy Market Trends and Growth 2025 to 2034
Claight Corporation
?
Digital Mitra Traininfsfsfsfsfsdffffffff
Digital Mitra Traininfsfsfsfsfsdffffffff
SyedRajaAli
?
Malt based foods nd fermented Products.pptx
Malt based foods nd fermented Products.pptx
khantooba325
?
food allergy and food intolarance (1) (1).pptx
food allergy and food intolarance (1) (1).pptx
z6znrhjj6h
?
2024 Trend Updates: What Really Works In SEO & Content Marketing
2024 Trend Updates: What Really Works In SEO & Content Marketing
Search Engine Journal
?
Storytelling For The Web: Integrate Storytelling in your Design Process
Storytelling For The Web: Integrate Storytelling in your Design Process
Chiara Aliotta
?

More Related Content

Recently uploaded (20)

nomadic empire.pdfgreegxwegrhcwrthchthcerh
nomadic empire.pdfgreegxwegrhcwrthchthcerh
ParthChauhan522968
?
How to Maintain Garden for Growing Seeds.ppt
How to Maintain Garden for Growing Seeds.ppt
Adinath Trading Company
?
YZM Food Truck Catalog for those who are interested to start food truck. it h...
YZM Food Truck Catalog for those who are interested to start food truck. it h...
tahirrasheid
?
WEEK 1(cookery-subject orientation).pptx
WEEK 1(cookery-subject orientation).pptx
sharlene sacristia
?
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeffrey Kaliel
?
Title Lorem Ipsum.pptx math tasty coffee
Title Lorem Ipsum.pptx math tasty coffee
cdiem040
?
Basic forms of matter and energy Matter and energy as a resource Scientific l...
Basic forms of matter and energy Matter and energy as a resource Scientific l...
rakesh921395
?
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
Aman Bansal
?
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
taqyed
?
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Sarvesh Bose
?
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
taqyea
?
Lecture 5.2 Understanding Demographic Change .pptx
Lecture 5.2 Understanding Demographic Change .pptx
shofalbsb
?
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
Taqyea
?
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
naitikbansal61
?
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
pegjpope
?
Anemia Presentation, introduction, causes (1) (2).pptx
Anemia Presentation, introduction, causes (1) (2).pptx
z6znrhjj6h
?
South Korea Dairy Market Trends and Growth 2025 to 2034
South Korea Dairy Market Trends and Growth 2025 to 2034
Claight Corporation
?
Digital Mitra Traininfsfsfsfsfsdffffffff
Digital Mitra Traininfsfsfsfsfsdffffffff
SyedRajaAli
?
Malt based foods nd fermented Products.pptx
Malt based foods nd fermented Products.pptx
khantooba325
?
food allergy and food intolarance (1) (1).pptx
food allergy and food intolarance (1) (1).pptx
z6znrhjj6h
?
nomadic empire.pdfgreegxwegrhcwrthchthcerh
nomadic empire.pdfgreegxwegrhcwrthchthcerh
ParthChauhan522968
?
How to Maintain Garden for Growing Seeds.ppt
How to Maintain Garden for Growing Seeds.ppt
Adinath Trading Company
?
YZM Food Truck Catalog for those who are interested to start food truck. it h...
YZM Food Truck Catalog for those who are interested to start food truck. it h...
tahirrasheid
?
WEEK 1(cookery-subject orientation).pptx
WEEK 1(cookery-subject orientation).pptx
sharlene sacristia
?
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeff Kaliel_ Reviving the Heartland Through Sustainable Agriculture.pdf
Jeffrey Kaliel
?
Title Lorem Ipsum.pptx math tasty coffee
Title Lorem Ipsum.pptx math tasty coffee
cdiem040
?
Basic forms of matter and energy Matter and energy as a resource Scientific l...
Basic forms of matter and energy Matter and energy as a resource Scientific l...
rakesh921395
?
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
High-pressure Processing (HPP) Foods Market Trends and Growth 2034
Aman Bansal
?
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
Î÷°àÑÀ¬ɭ±¤ÉÌѧԺ³É¼¨µ¥Ë®Ó¡µ÷³¢³§µþ±ÏÒµÍê³ÉÐų¢³§µþѧÀúÈÏÖ¤·¶±¾°¨ÈçºÎÖÆ×÷
taqyed
?
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Exploring Ghanaian Cuisine and Its Unique Flavours Rooted in Culture and Trad...
Sarvesh Bose
?
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹ú¿µÄζû´óѧ±ÏÒµÖ¤£¨°ä±«±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
taqyea
?
Lecture 5.2 Understanding Demographic Change .pptx
Lecture 5.2 Understanding Demographic Change .pptx
shofalbsb
?
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
×îаæÃÀ¹úÌØÀ­»ª´óѧ±ÏÒµÖ¤£¨±«¶Ù±ð±ô±ÏÒµÖ¤Ê飩ԭ°æ¶¨ÖÆ
Taqyea
?
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
pdfresizer.com-pdf-resize (2).pdfajhdbcnc
naitikbansal61
?
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
Boyle_CommunityNutritionInAction_8e_PPT_Chapter11.pptx
pegjpope
?
Anemia Presentation, introduction, causes (1) (2).pptx
Anemia Presentation, introduction, causes (1) (2).pptx
z6znrhjj6h
?
South Korea Dairy Market Trends and Growth 2025 to 2034
South Korea Dairy Market Trends and Growth 2025 to 2034
Claight Corporation
?
Digital Mitra Traininfsfsfsfsfsdffffffff
Digital Mitra Traininfsfsfsfsfsdffffffff
SyedRajaAli
?
Malt based foods nd fermented Products.pptx
Malt based foods nd fermented Products.pptx
khantooba325
?
food allergy and food intolarance (1) (1).pptx
food allergy and food intolarance (1) (1).pptx
z6znrhjj6h
?

Featured (20)

2024 Trend Updates: What Really Works In SEO & Content Marketing
2024 Trend Updates: What Really Works In SEO & Content Marketing
Search Engine Journal
?
Storytelling For The Web: Integrate Storytelling in your Design Process
Storytelling For The Web: Integrate Storytelling in your Design Process
Chiara Aliotta
?
Artificial Intelligence, Data and Competition ¨C SCHREPEL ¨C June 2024 OECD dis...
Artificial Intelligence, Data and Competition ¨C SCHREPEL ¨C June 2024 OECD dis...
OECD Directorate for Financial and Enterprise Affairs
?
How to Leverage AI to Boost Employee Wellness - Lydia Di Francesco - SocialHR...
How to Leverage AI to Boost Employee Wellness - Lydia Di Francesco - SocialHR...
SocialHRCamp
?
2024 State of Marketing Report ¨C by Hubspot
2024 State of Marketing Report ¨C by Hubspot
Marius Sescu
?
Everything You Need To Know About ChatGPT
Everything You Need To Know About ChatGPT
Expeed Software
?
Product Design Trends in 2024 | Teenage Engineerings
Product Design Trends in 2024 | Teenage Engineerings
Pixeldarts
?
How Race, Age and Gender Shape Attitudes Towards Mental Health
How Race, Age and Gender Shape Attitudes Towards Mental Health
ThinkNow
?
AI Trends in Creative Operations 2024 by Artwork Flow.pdf
AI Trends in Creative Operations 2024 by Artwork Flow.pdf
marketingartwork
?
Skeleton Culture Code
Skeleton Culture Code
Skeleton Technologies
?
PEPSICO Presentation to CAGNY Conference Feb 2024
PEPSICO Presentation to CAGNY Conference Feb 2024
Neil Kimberley
?
Content Methodology: A Best Practices Report (Webinar)
Content Methodology: A Best Practices Report (Webinar)
contently
?
How to Prepare For a Successful Job Search for 2024
How to Prepare For a Successful Job Search for 2024
Albert Qian
?
Social Media Marketing Trends 2024 // The Global Indie Insights
Social Media Marketing Trends 2024 // The Global Indie Insights
Kurio // The Social Media Age(ncy)
?
Trends In Paid Search: Navigating The Digital Landscape In 2024
Trends In Paid Search: Navigating The Digital Landscape In 2024
Search Engine Journal
?
5 Public speaking tips from TED - Visualized summary
5 Public speaking tips from TED - Visualized summary
SpeakerHub
?
ChatGPT and the Future of Work - Clark Boyd
ChatGPT and the Future of Work - Clark Boyd
Clark Boyd
?
Getting into the tech field. what next
Getting into the tech field. what next
Tessa Mero
?
Google's Just Not That Into You: Understanding Core Updates & Search Intent
Google's Just Not That Into You: Understanding Core Updates & Search Intent
Lily Ray
?
How to have difficult conversations
How to have difficult conversations
Rajiv Jayarajah, MAppComm, ACC
?
2024 Trend Updates: What Really Works In SEO & Content Marketing
2024 Trend Updates: What Really Works In SEO & Content Marketing
Search Engine Journal
?
Storytelling For The Web: Integrate Storytelling in your Design Process
Storytelling For The Web: Integrate Storytelling in your Design Process
Chiara Aliotta
?
How to Leverage AI to Boost Employee Wellness - Lydia Di Francesco - SocialHR...
How to Leverage AI to Boost Employee Wellness - Lydia Di Francesco - SocialHR...
SocialHRCamp
?
2024 State of Marketing Report ¨C by Hubspot
2024 State of Marketing Report ¨C by Hubspot
Marius Sescu
?
Everything You Need To Know About ChatGPT
Everything You Need To Know About ChatGPT
Expeed Software
?
Product Design Trends in 2024 | Teenage Engineerings
Product Design Trends in 2024 | Teenage Engineerings
Pixeldarts
?
How Race, Age and Gender Shape Attitudes Towards Mental Health
How Race, Age and Gender Shape Attitudes Towards Mental Health
ThinkNow
?
AI Trends in Creative Operations 2024 by Artwork Flow.pdf
AI Trends in Creative Operations 2024 by Artwork Flow.pdf
marketingartwork
?
PEPSICO Presentation to CAGNY Conference Feb 2024
PEPSICO Presentation to CAGNY Conference Feb 2024
Neil Kimberley
?
Content Methodology: A Best Practices Report (Webinar)
Content Methodology: A Best Practices Report (Webinar)
contently
?
How to Prepare For a Successful Job Search for 2024
How to Prepare For a Successful Job Search for 2024
Albert Qian
?
Trends In Paid Search: Navigating The Digital Landscape In 2024
Trends In Paid Search: Navigating The Digital Landscape In 2024
Search Engine Journal
?
5 Public speaking tips from TED - Visualized summary
5 Public speaking tips from TED - Visualized summary
SpeakerHub
?
ChatGPT and the Future of Work - Clark Boyd
ChatGPT and the Future of Work - Clark Boyd
Clark Boyd
?
Getting into the tech field. what next
Getting into the tech field. what next
Tessa Mero
?
Google's Just Not That Into You: Understanding Core Updates & Search Intent
Google's Just Not That Into You: Understanding Core Updates & Search Intent
Lily Ray
?
Ad

Iafp poster 072916 pdf