In the zone
- 1. Report of Results with Discussion
- 2. 6 Bacterial Strains 6 Antibacterial Agents Hypothesis:That all of the antiseptic and disinfectant agents will be effective against all of the bacterial strains
- 3. 10% Bleach Hand Sanitizer Spic & Span Mouthwash Rubbing AlcoholHydrogen Peroxide Sensi-disk Agar Plate
- 5. AntibacterialAgent Zone of Inhibition (mm) Bacterial Strain E. Coli S. Aureus P. aerugin P.Vulgaris B. Cereus E. Faecilis Hand Sanitizer 12.5 0 0 0 0 0 Hydrogen Peroxide 1 15.5 2 14 9 15 Mouth Wash 0 0 0 0 0 0 Rubbing Alcohol 0 0 0 0 0 0 10% Bleach 0 0 1 3 0 2 Spic & Span 2 2 0 0 6.5 0
- 6. 0 2 4 6 8 10 12 14 16 18 E. Coli S. Aureus P. aerugin P. Vulgaris B. Cereus E. Faecilis DiameterofZoneofInhibition(mm) Bacterial Strain Hand Sanitizer Hydrogen Peroxide Mouth Wash Rubbing Alcohol 10% Bleach Spic & Span
- 7. Hydrogen Peroxide was, comparatively, the most consistently and highly effective antibacterial agent tested Spic and Span as well as bleach come in second and third Average Zones of Inhibition AntibacterialAgent Average Zone of Inhibition Hand Sanitizer 2.1 Hydrogen Peroxide 9.4 Mouth Wash 0.0 RubbingAlcohol 0.0 10% Bleach 1.0 Spic & Span 1.75
- 8. Antibacterial Agent Average Zone of Inhibition Consistency Coefficient (# of bacterial strains effective against/total bacterial strains) Total Efficacy Rating ((Coefficient) ( Avg. Zone)) Hand Sanitizer 2.1 0.17 0.35 Hydrogen Peroxide 9.4 1 9.42 MouthWash 0.0 0 0.00 Rubbing Alcohol 0.0 0 0.00 10% Bleach 1.0 0.5 0.50 Spic & Span 1.75 0.5 0.88
- 9. ?
- 10. Lab Group Kelly Cinelli Eric Carpizo Dan Dehelian Lab Instructor Dr. E. Dawley T.A. Chris Frymoyer UC Biology Department
- 11. Image Credits in Order of Appearance Bacteria pictures: http://www.freedrinkingwater.com/Images-Test/E-Coli01.jpg http://www.vanderbilt.edu/exploration/resources/staph_aureus_800.jpg http://pseudomonas.com/images/paeruginosa.jpg http://www.ciriscience.org/thumbimage.php?id=155 https://encrypted-tbn0.google.com/images?q=tbn:ANd9GcT2cuGZgkF4U7SA7yRW3HDoopkUlbqd- FVmuN6AlHIarWFIJJiYXA http://upload.wikimedia.org/wikipedia/en/thumb/4/4a/Enterococcus_faecalis20023-300.jpg/220px- Enterococcus_faecalis20023-300.jpg Antibacterial Pictures: https://encryptedtbn2.google.com/images?q=tbn:ANd9GcSfNvZpXJob0xfyOAzGAO1NQdtMSAb5IGrU 6TpxE0jc89A1RiYn http://substuff.files.wordpress.com/2010/11/spicspancleaner.jpg http://www.germx.com/data/photos/8oz_Aloe.jpg http://www.mcguffmedical.com/Images/Images550/003208%20Hydrogen%20Peroxide%203%20Percen t,%20Topical%20USP,%2016%20Ounce%20Bottle,%20Each%20McGuffMedical.com.jpg http://www.scopemouthwash.com/images/crest-products/large/scope-white-mouthwash-mint- splash.png http://maryl.uibcsites.com/files/2011/05/Alcohol-Isopropyl.jpg Zone of Inhibition Diagram: Ursinus Bio Department Lab 4 instructions
- 12. Lab 2.3 & 2.4 Procedure from the Ursinus Bio Department Material Consulted for Discussion Hand Gels Falter C. B. Science News ,Vol. 169, No. 23 (Jun. 10, 2006), p. 366 http://zack2.ursinus.edu:2075/stable/pdfplus/10.1086/503455.pdf?acceptTC=true Efficacy of AlcoholBased Hand SanitizersAgainst Fungi andViruses E. Fendler and P. Groziak InfectionControl and Hospital Epidemiology ,Vol. 23, No. 2 (February 2002), pp. 61-62
Editor's Notes
- #8: As it had at least some zone of inhibition against each bacterial strain, as well as the largest zone of inhibition in all cases but one Spic and Span is effective in that it inhibited the growth of colonies in one half of the bacterial strains tested, and Bleach also inhibited colony growth It can be seen in the average zones of inhibition table that Hydrogen peroxide had the largest average zone of inhibition, but that hand sanitizer has the second highest average may be deceiving, because it is less consistent than bleach and spic and span, as it only produced a zone of inhibition against E. Coli bacteria, and because it was a relatively large zone comparative to other results from this experiment, its average is not representative of the overall efficacy.
- #10: Mouthwash is designed to fight oral bacteria, whereas the other antibacterials are more general in use, so that could explain its ineffectiveness These three agents share alcohol as an active ingredient