Agrobacterium detects wounded plant cell phenolics using its VirA/VirG two component sensor system, which induces expression of virulence genes. This allows VirD1 and VirD2 to cut T-DNA from the bacterial plasmid at the right and left borders. VirD2 then attaches to the exposed 5' end of T-DNA, and associates with VIP1 and VIP2 to target the complex to the plant cell nucleus. There, the T-DNA integrates into the plant genome and initiates gall formation.
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Infection process of agrobacterium
1. Phenolics
Produced by
Wounded
Plant cell
PLANT CELL
AGROBACTERIUM
VirA VirG
Phenolics
detected by the
VirA/VirG two
component
sensor system.
Vir Gene
expression
induced
T-DNA
Vir D1 & D2 cut
T-DNA at right
and left borders.
VirD2 attaches to
exposed 5I
end
VIP1 associates with
the complex to target
it to the nucleus
VIP2 associates the
complex to
transcriptionally
active DNAT-DNA integrates into plant DNA
and gall production is initiated.
Gall Formation!
Formation of T-
complex
Bacterial
Plasmid
Formation of T-Pilus