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Lab+3 convertido

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Rocio Rimac Valdivia
Rocio Rimac Valdivia

1. Hematocrit (Hct) measures the percentage of red blood cells in blood after centrifugation. Blood is centrifuged to pack the red blood cells below and plasma above using centrifugal force. 2. The Hct is calculated by measuring the height of the packed red blood cell column and dividing by the total height of the blood column in a capillary tube, then multiplying by 100. 3. A normal Hct range is 36-46% for females and 40-54% for males. Factors like centrifugation speed and time can affect the Hct results if not done properly. The Hct is used to screen for anemia but hemoglobin is a more significant indicator.

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R E E M A L S OL A MI Hematocrit (Hct) (Packed cell Volume) (PCV)
Hct/ PCV Objective: To pack the RBC using the centrifuge force. Forcing all red cell below and plasma above, by centrifugal force.
Defenition of Hct The percentage by volume of packed red blood cells in a given sample of blood after centrifugation
Purpose for doing the Hct Blood is made up of red and white blood cells, and plasma. A decrease in the number or size of red cells also decreases the amount of space they occupy, resulting in a lower hematocrit. An increase in the number or size of red cells increases the amount of space they occupy, resulting in a higher hematocrit.
Procedure 1. Capillary tube is used
2. The capillary is filled with blood to half of the tube , but not filled too much
3. Sealing the capillary with clay (cement)
4. Centrifugation (10000 rpm ad two minute)
5. Reading the Hct
By Ruler PCV (Hct)= Height of RBC column (cm) x 100 Total Height of blood column(cm) Two way to express your result: With x100 without point Without x 100 with point
Result Female 0.36-0.46 Male 0.40-0.54 At birth 0.44-0.54 2-12 0.34-0.45 If multiplied by 100 e.g. 36-46
Factors affecting the Hct 1. Speed of centrifuge Ideal speed approximately 10000 rpm for two minutes. Higher than ideal speed ( rpm)-leads to Insufficient packing Falsely Higher volume of RBC Falsely Lower Hct Lower than ideal speed ( rpm)--leads to Lysis of RBC-- Lower RBc volume Falsely higher Hct
2.Time of centrifugation Under centrifugation: Time insufficient packing of RBC Falsely higher Hct. Over centrifugatiuon: Time RBC lysis- Falsely lower Hct.
3. Proper sealing Improper sealing: 1Big hole-- leaking of whole blood and empty the capillary while centrifuged. 2Very small hole (unseen by eye)--causes small leak that only affects the RBC volume because it closer to the sealing-- falsely low RBC volume- low Hct.
4. Biological factors Trapped plasma 1. In normal condition: There is a small per cent of plasma which is trapped with the packed RBC, approximately 2-3%. E.g. 43 is actually 40 by considering the 3% of plasma , but it is always be ignored as it is negligible.
2. In pathological condition: The per cent of trapped plasma may increased , reaching to 10% , and thus leads to high RBC volume and ultimately leads to high Hct.
Medical advantage of Hct The hematocrit is used to screen for anaemia, or is measured on a person to determine the extent of anaemia A result within reference range doesnt necessary mean that patient is not anaemic, and vice versa. Haemoglobin concentration is more significant for anaemia diagnosis.
Important Reminders Some students didnt wright their name and no reported result for them. Always write your name in the upper left corner With the number of lab under it Please always use proper A4 size paper and not a notebook. No one will be signed for attendance except after I see her clean place in the lab, unclean bench will reduce your mark.

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Lab+3 convertido

  • 1. R E E M A L S OL A MI Hematocrit (Hct) (Packed cell Volume) (PCV)
  • 2. Hct/ PCV Objective: To pack the RBC using the centrifuge force. Forcing all red cell below and plasma above, by centrifugal force.
  • 3. Defenition of Hct The percentage by volume of packed red blood cells in a given sample of blood after centrifugation
  • 4. Purpose for doing the Hct Blood is made up of red and white blood cells, and plasma. A decrease in the number or size of red cells also decreases the amount of space they occupy, resulting in a lower hematocrit. An increase in the number or size of red cells increases the amount of space they occupy, resulting in a higher hematocrit.
  • 6. 2. The capillary is filled with blood to half of the tube , but not filled too much
  • 7. 3. Sealing the capillary with clay (cement)
  • 8. 4. Centrifugation (10000 rpm ad two minute)
  • 10. By Ruler PCV (Hct)= Height of RBC column (cm) x 100 Total Height of blood column(cm) Two way to express your result: With x100 without point Without x 100 with point
  • 11. Result Female 0.36-0.46 Male 0.40-0.54 At birth 0.44-0.54 2-12 0.34-0.45 If multiplied by 100 e.g. 36-46
  • 12. Factors affecting the Hct 1. Speed of centrifuge Ideal speed approximately 10000 rpm for two minutes. Higher than ideal speed ( rpm)-leads to Insufficient packing Falsely Higher volume of RBC Falsely Lower Hct Lower than ideal speed ( rpm)--leads to Lysis of RBC-- Lower RBc volume Falsely higher Hct
  • 13. 2.Time of centrifugation Under centrifugation: Time insufficient packing of RBC Falsely higher Hct. Over centrifugatiuon: Time RBC lysis- Falsely lower Hct.
  • 14. 3. Proper sealing Improper sealing: 1Big hole-- leaking of whole blood and empty the capillary while centrifuged. 2Very small hole (unseen by eye)--causes small leak that only affects the RBC volume because it closer to the sealing-- falsely low RBC volume- low Hct.
  • 15. 4. Biological factors Trapped plasma 1. In normal condition: There is a small per cent of plasma which is trapped with the packed RBC, approximately 2-3%. E.g. 43 is actually 40 by considering the 3% of plasma , but it is always be ignored as it is negligible.
  • 16. 2. In pathological condition: The per cent of trapped plasma may increased , reaching to 10% , and thus leads to high RBC volume and ultimately leads to high Hct.
  • 17. Medical advantage of Hct The hematocrit is used to screen for anaemia, or is measured on a person to determine the extent of anaemia A result within reference range doesnt necessary mean that patient is not anaemic, and vice versa. Haemoglobin concentration is more significant for anaemia diagnosis.
  • 18. Important Reminders Some students didnt wright their name and no reported result for them. Always write your name in the upper left corner With the number of lab under it Please always use proper A4 size paper and not a notebook. No one will be signed for attendance except after I see her clean place in the lab, unclean bench will reduce your mark.