LLTech -Imaging C Elegans Worm in 3D with Light-CT
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The document describes a new tomographic imaging technique called Light-CT that allows for fast, non-invasive 3D imaging of the C. Elegans worm. Worms are anesthetized, encapsulated in a gel, and placed between a slide and coverslip before being imaged with the Light-CT scanner at a resolution of 1um. The full 3D imaging of the worm can be acquired in about 2 minutes, allowing identification of internal anatomy like the pharynx, isthmus, and gonad arms. Light-CT provides optical sectioning and volumetric imaging capabilities superior to standard microscopy.
LLTech -Imaging C Elegans Worm in 3D with Light-CT
- 1. Tomographic non-invasive imaging of C. Elegans worm: LLTech Light-CT scannerSample preparation: worms are anesthetized and encapsulated in a gel
- 2. a gel layer is placed between slide and coverslip
- 3. the sample is imaged using standard Light-CT scanner procedureImaging:3Dtomographic resolution: 1袖m, non invasive, non destructive
- 4. 3D acquisition of whole worm: 2minutes (about 60 images, thickness 1袖m), allowing fast 3D optical biopsycoverslipEncapsulated wormsMicroscope slide
- 5. Light-CT allows fast identification of anatomy worm 1Image plane 1 @ 15袖m depthImage plane 2 @ 30袖m depth800袖m800袖mCorpuspharynxIsthmusPosterior bulbExcretory canalspermatheca1vulvaeggsovocytesspermatheca2gonad armintestineovocytes
- 6. Light-CT allows fast identification of anatomy worm 2Image plane 1 @ 15袖m depthImage plane 2 @ 30袖m depth800袖m800袖movocytesintestineBody wall - cuticlegonad armuterus
- 7. Light-CT allows full volumetric imaging in a few minutesLight-CT image: allows fine separation of structuresStandard microscope imageOptical sectioning:1袖m thickness imageLight-CT images at different depths reveal fine structural details
- 8. Light-CT allows fast, precise 3D rendering and volume exploration
- 9. Images with 40x objectiveMuch higherresolution
- 10. Zoom on different parts
- 11. Scanning through the sampleMovieobtainedwith 3 microns steps
- 12. 3D viewing