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Luminescence presentation

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The document discusses different types of luminescence including bioluminescence used by deep sea organisms. It then describes how luminol is used by crime scene investigators to detect trace amounts of blood, even if cleaning products were used. Finally, it mentions green fluorescent protein (GFP) which was used to win the 2008 Nobel Prize in Chemistry and is now used in medical research to track proteins in diseases like cancer and Alzheimer's.

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From The Deep Sea to the Crime Lab Luminescence
Light Electrons are energized and photons are released to make light The electrons in higher orbitals emit a photon when they fall back to their original orbital Our eyes pick up the photons being emitted by the light source
Chemoluminescence - light produced by a chemical reaction Bioluminescence - light produced by a living organism Fluorescence - when light is absorbed and re-emitted at a different wavelength Phosphorescence - when energy is absorbed and re-emitted but over along period of time These are all examples of COLD light Luminescence
Bioluminescence Used in 90% of deep sea organisms and a few terrestrial organisms Used to find prey, hide, and communicate Mostly blue and green but some red light as well -- Why?
Uses for Bioluminescence Counterillumination Use of red light to see prey Lure prey Stun or confuse predators or prey Attract a mate Communication
How does it work?
Enzyme
Luciferins
So... We have seen how organisms can use luminescence... How can we use luminescence?
Luminol Used by crime scene investigators to detect blood The iron in blood acts like a catalyst to activate luminol, producing light Helpful in finding blood that may have been cleaned up because traces are left behind Bleach will react with luminol, showing if cleaning product has been used
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Green Fluorescent Protein Aequorea victoria Aequorin is the protein, which emits blue light when bound with calcium GFP absorbs the blue light and makes it green under UV light Osamu Shimomura, Marty Chalfie and Roger Tsien received the Nobel Prize in Chemistry in 2008 for their work with GFP Used so we can see where proteins are made and where they end up helpful in cancer, Alzheimer’s, and HIV research
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Brainbow

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Luminescence presentation

  • 1. From The Deep Sea to the Crime Lab Luminescence
  • 2. Light Electrons are energized and photons are released to make light The electrons in higher orbitals emit a photon when they fall back to their original orbital Our eyes pick up the photons being emitted by the light source
  • 3. Chemoluminescence - light produced by a chemical reaction Bioluminescence - light produced by a living organism Fluorescence - when light is absorbed and re-emitted at a different wavelength Phosphorescence - when energy is absorbed and re-emitted but over along period of time These are all examples of COLD light Luminescence
  • 4. Bioluminescence Used in 90% of deep sea organisms and a few terrestrial organisms Used to find prey, hide, and communicate Mostly blue and green but some red light as well -- Why?
  • 5. Uses for Bioluminescence Counterillumination Use of red light to see prey Lure prey Stun or confuse predators or prey Attract a mate Communication
  • 6. How does it work?
  • 9. So... We have seen how organisms can use luminescence... How can we use luminescence?
  • 10. Luminol Used by crime scene investigators to detect blood The iron in blood acts like a catalyst to activate luminol, producing light Helpful in finding blood that may have been cleaned up because traces are left behind Bleach will react with luminol, showing if cleaning product has been used
  • 11. Ìý
  • 12. Green Fluorescent Protein Aequorea victoria Aequorin is the protein, which emits blue light when bound with calcium GFP absorbs the blue light and makes it green under UV light Osamu Shimomura, Marty Chalfie and Roger Tsien received the Nobel Prize in Chemistry in 2008 for their work with GFP Used so we can see where proteins are made and where they end up helpful in cancer, Alzheimer’s, and HIV research
  • 13. Ìý