MAG
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This document evaluates the antimicrobial properties of monoacylglycerols (MAG) and nisin. It describes preparing MAG from lauric acid, palmitic acid, and adamantanecarboxylic acid. Methods include testing the MAG and nisin individually and combined against various gram-positive and gram-negative bacteria using growth curve analysis. Some results showed MAG inhibiting Bacillus subtilis by 41%. Problems included equipment issues. The conclusion suggests a synergistic effect between nisin and MAG, and that combining them could replace part of the nisin to reduce costs.
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MAG
- 1. Evaluation of antimicrobial properties of MAGGabriel Costa09/11/2009
- 3. Introduction
- 4. IntroductionMonoacylglycerolsCARACHTERISTICS Partial esters of glycerol
- 5. Non-ionic surface-active compounds
- 6. BiodegradabilityAPPLICATION Food, cosmetic, textile, plastic industry, etc.PREPARATION Interesterification
- 7. Hydrolysis of triacylglycerols
- 8. Fatty acid addition to glycidolIntroductionNisinCARACHTERISTICS Polycyclic peptide油antibacterial with 34 aminoacids
- 9. 油Rare example of a broad-spectrum bacteriocin (effective against gram positives and gram negatives)APPLICATION Foodindustry as foodpreservativePREPARATION Fermentation usingLactococcuslactisAim
- 10. Evaluate油antimicrobial effect油of油monoacylglycerol油LauricacidPalmitic acidAdamantanecarboxylic acidAimNisin
- 11. Evaluate油of油antimicrobial effect油of油monoacylglycerol油LauricacidPalmitic acidAdamantanecarboxylic acidAimMAG ANisin
- 12. Methods
- 13. 1-Adamantanecarboxylic acid + toluene (8ml per 1 gram)Lauric acid or palmiticacidglass reactor with a temperature-stabilized jacket96 C 30 minutesGlycidol acid:glycidol (1:1,8)Purification and FiltrationMethodsPreparation of MAGSolvent systemNon-solvent system油Chromium (III) acetate hydroxide0,5% w/w96 C 30 minutes96 C 120 minutes
- 14. MethodsAntimicrobialassayBacillus subtilisStaphylococcus aureusBacillus cereusMicrococcus luteusEnterococcusFaecalisGram +Micrococcus luteus CCM 732Escherichia coliCitrobacterfreundiiSerratiamarcescensSalmonella entericaPseudomonas aeruginosaGram -Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420
- 15. MethodsAntimicrobialassayBacillus subtilisStaphylococcus aureusBacillus cereusMicrococcus luteusEnterococcusFaecalisGram +Micrococcus luteus CCM 732Escherichia coliCitrobacterfreundiiSerratiamarcescensSalmonella entericaPseudomonas aeruginosaGram -Salmonella enterica ssp. Enterica ser. Enteritidis CCM 4420
- 16. MethodsAntimicrobialassay油Dilution of MAG in MPB(25 - 1500 袖l/ml)油200袖l MPB with MAG + 5 袖l of bacterial suspensionIncubation in plate readerAbsorbance readings (600nm)Every 30 minutes24 hoursGrowth curves
- 17. Results
- 18. ResultsGram +
- 19. ResultsGram +
- 20. ResultsGram +
- 24. ResultsPetridishesUsed to confirmtheresultsMAG A of Iva-1000袖g/mlMAG A of Gabriel - 1000 袖g/mlNisin - 500袖g/mlMAG A of Iva + Nisin100MAG + 300N 袖g/mlMAG A of Gabriel + Nisin-100MAG + 300N 袖g/mlMAG Lauric - 1000袖g/mlMAG A of Gabriel + Nisin - 200MAG + 200N 袖g/ml
- 25. Problems
- 26. ProblemsWealwayshave...MAG ADidnot work thefirst time
- 27. 10 days to realize and prepare anotheronePLATE READERLight bulbburned out
- 28. Couldnottest MAG of palmiticacid
- 29. Couldnottest mix (Nisin + MAG) in gramnegativebacteriasConclusions
- 32. Part of nisincouldbereplacedby MAG to reducecostsAcknowledgments