The Mc'Fadyean Reaction is a staining technique used to identify Anthrax bacillus under a microscope by staining the polypeptide capsular material around the bacilli that is composed of poly-d-Glutamic Acid. The procedure involves making an impression slide from an animal's ear, staining it with Poly Chrome Methylene Blue for 30 seconds, and examining under a microscope, where a positive sample will show a purplish capsule material surrounding the blue stained bacilli bodies, presumptively diagnosing anthrax in animals.
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2. INTRODUCTION
Used to identify Anthrax bacillus in microscope.
Actually a Staining technique.
Employed for the presumptive diagnosis of anthrax in animals.
Stains the polypeptide capsular material of anthrax bacilli that is composed
of poly-dGlutamic Acid.
3. PROCEDURE
Take blood from tip of ear and make a impression slide.
This is stained with Poly Chrome Methylene Blue (PCMB) for 30 seconds.
Examine under microscope.
If the sample is positive, an amorphous purplish material is noticed around
the blue bacilli.
This purplish material is capsule around blue bacilli body.
