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Microbial genomics tool beta version 1

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Rajendra Verma
Rajendra Verma

This document describes a microbial genomics tool in beta version 1.0 created by Mr. Rajendra Kumar Verma and Mr. Kishore Shende Sir. The tool takes a FASTA format input file, which is a text-based format used to represent nucleotide or peptide sequences. It provides outputs in both text and Microsoft Excel formats, including nucleotide composition, amino acid composition, and other metrics.

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M ICROBIAL G ENOMICS TOOL B ETA V ERSION 1.0 By Mr. Rajendra kumar Verma & Mr. Kishore Shende Sir
TAKE INPUT FASTA FILE ? In bioinformatics, FASTA format is a text- based format for representing either nucleotide sequences or peptide sequences, in which nucleotides or amino acids are represented using single-letter codes. The format also allows for sequence names and comments to precede the sequences. The format originates from the FASTA software package, but has now become a standard in the field of bioinformatics. ? A sequence in FASTA format begins with a single-line description, followed by lines of sequence data. The description line is distinguished from the sequence data by a greater-than (">") symbol in the first column. The word following the ">" symbol is the identifier of the sequence, and the rest of the line is the description (both are optional). There should be no space between the ">" and the first letter of the identifier. It is recommended that all lines of text be shorter than 80 characters. The sequence ends if another line starting with a ">" appears; this indicates the start of another sequence. A simple example of one sequence in FASTA format:
E XAMPLE OF FASTA FILE
T HIS IS A TOOL It gives output in text format and M.S Excel format in Frame and M.S Excel file.
Nucleotide Composition 400 300 80 200 Nucleotide 60 100 Compositio 40 n Series1 0 20 A T G C 0 (A+T)% (G+C)% 20 Axis Title 15 30 10 25 5 20 0 15 1 10 Series1 Amino Acid 0.8 5 0 0.6 Nc ENc 0.4 Series1 0.2 Axis Title 0 GC3s AT3s
Microbial genomics tool beta version 1

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Microbial genomics tool beta version 1

  • 1. M ICROBIAL G ENOMICS TOOL B ETA V ERSION 1.0 By Mr. Rajendra kumar Verma & Mr. Kishore Shende Sir
  • 2. TAKE INPUT FASTA FILE ? In bioinformatics, FASTA format is a text- based format for representing either nucleotide sequences or peptide sequences, in which nucleotides or amino acids are represented using single-letter codes. The format also allows for sequence names and comments to precede the sequences. The format originates from the FASTA software package, but has now become a standard in the field of bioinformatics. ? A sequence in FASTA format begins with a single-line description, followed by lines of sequence data. The description line is distinguished from the sequence data by a greater-than (">") symbol in the first column. The word following the ">" symbol is the identifier of the sequence, and the rest of the line is the description (both are optional). There should be no space between the ">" and the first letter of the identifier. It is recommended that all lines of text be shorter than 80 characters. The sequence ends if another line starting with a ">" appears; this indicates the start of another sequence. A simple example of one sequence in FASTA format:
  • 3. E XAMPLE OF FASTA FILE
  • 4. T HIS IS A TOOL It gives output in text format and M.S Excel format in Frame and M.S Excel file.
  • 5. Nucleotide Composition 400 300 80 200 Nucleotide 60 100 Compositio 40 n Series1 0 20 A T G C 0 (A+T)% (G+C)% 20 Axis Title 15 30 10 25 5 20 0 15 1 10 Series1 Amino Acid 0.8 5 0 0.6 Nc ENc 0.4 Series1 0.2 Axis Title 0 GC3s AT3s