This document summarizes the structure and function of mitochondria. It describes the four main parts of mitochondria: the outer membrane, intermembrane space, inner membrane, and matrix. It explains that the inner membrane has convolutions called cristae where oxidative phosphorylation occurs, removing electrons from NADH and FADH2 to pump hydrogen ions from the matrix to the intermembrane space. This creates a pH and electrical gradient across the inner membrane. ATP synthase then uses this gradient to phosphorylate ADP and produce ATP in the matrix. The process of using this proton gradient to generate ATP is called chemiosmosis.
2. Parts of the MitochondriaParts of the Mitochondria
Outer MembraneOuter Membrane
Intermembrane SpaceIntermembrane Space
Inner MembraneInner Membrane
MatrixMatrix
3. Like the plasma membrane
Consists of double
layer of phospholipids
4. Intermembrane SpaceIntermembrane Space
Narrow area between the inner andNarrow area between the inner and
outer membranesouter membranes
HH++
ions (protons) accumulate here.ions (protons) accumulate here.
5. Also double phospholipid layerAlso double phospholipid layer
Oxidative phosphorylation occurs hereOxidative phosphorylation occurs here
Has convolutions called cristaeHas convolutions called cristae
The electron transports removes electrons from NADH andThe electron transports removes electrons from NADH and
FADHFADH22 HH++
ions from the matrix to the intermembrane spaceions from the matrix to the intermembrane space
Another protein complex, ATP synthase, is responsible forAnother protein complex, ATP synthase, is responsible for
the phosphorylation of ADP to form ATPthe phosphorylation of ADP to form ATP
6. Fluid material that fills the area inside the innerFluid material that fills the area inside the inner
membrane.membrane.
The Krebs cycle and the conversion of pyruvate toThe Krebs cycle and the conversion of pyruvate to
acetyl CoA occurs here.acetyl CoA occurs here.
MatriMatri
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7. Chemiosmosis inChemiosmosis in
MitochondriaMitochondria
Oxidation PhosphorylationOxidation Phosphorylation
Krebs Cycle produce NADH and FADHKrebs Cycle produce NADH and FADH22
Electrons are removed from NADH andElectrons are removed from NADH and
FADHFADH22
H+ ions are transported to intermembraneH+ ions are transported to intermembrane
pH and electrical gradient are createdpH and electrical gradient are created
ATP sythase generates ATPATP sythase generates ATP
8. Oxidation PhosporylationOxidation Phosporylation
in Mitochondriain Mitochondria
Krebs cycle produces NADH and FADHKrebs cycle produces NADH and FADH22 in the matrix, COin the matrix, CO22 isis
generated in process of producing ATP.generated in process of producing ATP.
Protein complex in inner membrane removes electrons from NADHProtein complex in inner membrane removes electrons from NADH
and FADHand FADH22
Protein complexes transport HProtein complexes transport H++
ions form matrix to theions form matrix to the
intermembrane space.intermembrane space.
A pH and electrical gradient are created across the innerA pH and electrical gradient are created across the inner
membrane by Hmembrane by H++
into the intermembrane space.into the intermembrane space.CC
Channel Protein assist ATP synthase by allowing protons inChannel Protein assist ATP synthase by allowing protons in
intermembrane to flow back into matrix.intermembrane to flow back into matrix.