Mohamed Ezzat El Zowalaty avian influenza PhD_thesis results
•
2 likes•596 views
This document contains results from several RT-PCR tests performed on avian samples, including hemagglutination assays to detect avian influenza virus (AIV), RT-PCR tests targeting the AIV matrix, HA, and NA genes to identify subtypes, and RT-PCR for the Newcastle disease virus (NDV) F gene. Gel electrophoresis images show amplification products of the expected sizes for AIV and NDV genes. Tests were also able to distinguish AIV positive and negative samples, as well as AIV and NDV co-infected samples treated with NDV antiserum.
Mohamed Ezzat El Zowalaty avian influenza PhD_thesis results
- 1. 1 2 3 4 5 6 7 8 9 10 11 12 HA positive HA negative Positive control wells (A 12 to D12) Negative control wells (E 12 to H12) A B C D E F G H Hemagglutination test
- 2. AIV positve sample AIV negative sample Avian influenza rapid antigen detection test
- 3. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M Subtyping of AIV cloacal samples using -RT-PCR-degenerate primers 1550 HA (1778 bp) NA (1413 bp) 1400 2000 1000 750 M (1027 bp) NSP (890 bp)
- 4. Subtyping of AIV cloacal samples using -RT-PCR-degenerate primers 2000 1550 1400 1000 750 M 1 2 3 4 5 6 7 M
- 5. Subtyping of AIV cloacal samples using -RT-PCR-NA-Hoffmann primers 2000 1550 1400 1000 M 1 2 3 4 5 6 7 8 9 10 M NA (1413 bp)
- 6. Subtyping of AIV cloacal samples using -RT-PCR-NA-Hoffmann primers 2000 1550 1000 1400 M 1 2 3 4 5 6 7 8 9 10 M NA (1413 bp)
- 7. AIV Matrix-RT-PCR 1000 M 1 2 3 4 5 6 7 8 9 10 M 1400 M (1027 bp)
- 8. AIV Matrix-RT-PCR 1400 1000 M 1 2 3 4 5 6 7 8 9 10 M M (1027 bp)
- 9. AIV Matrix--RT-PCR M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M 1400 1000 M 1 2 3 4 5 6 7 8 9 10 11 12 M M (1027 bp)
- 10. Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers 2000 1400 1000 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M HA (1778 bp) M 1 2 3 4 5 6 7 1550
- 11. Cloacal samples AIV subtyping-RT-PCR-HA-Hoffmann primers M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M M 1 2 3 4 5 6 7 2000 1400 1000 1550 HA (1778 bp)
- 12. 2000 1400 1000 1550 HA (1778 bp) Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers M 1 2 3 4 5 6 7
- 13. 2000 1400 1000 1550 HA (1778 bp) Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers M 1 2 3 4 M
- 14. 500 1000 750 HA (640 bp) Subtyping of AIV cloacal samples using RT-PCR-HA-Phipps primers M 1 2 3 4 5 6 7 M
- 15. 500 1000 750 HA (640 bp) Cloacal samples AIV subtyping-RT-PCR-HA-Phipps primers M 1 2 3 4 5 6 7 M
- 16. AIV HA and NA DNA after purification of the PCR bands for sequencing M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M 2000 1400 1000 1550 HA (1778 bp) NA (1413 bp) M 1 2 3 M 4 5 6 7 8 9 10 11 12 13 M
- 17. AIV-RT-PCR-H5-cleavege site M 1 2 3 4 300 400 HA cleavage site (249 bp) 200
- 18. AIV-RT-PCR-H7-cleavege site M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M 300 400 200 HA cleavage site (241bp) M 1 2 3 4
- 19. AIV subtyping-RT-PCR-NA.2.5.Chander primers 500 M 1 2 3 4 5 6 7 8 9 10 11 12 M NA (536 bp)
- 20. AIV subtyping-RT-PCR-NA.8.1.Chander primers M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M 500 NA (429 bp) M 1 2 3 4 5 6 7 8 9 10 11 12 M
- 21. AIV sub typing RT-PCR-NA 6.1.Chander primers 500 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 M NA (442 bp)
- 22. NDV RT-PCR 400 300 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M NDV F gene (340 bp) M 1 2 3 4 5 6 7 8 9 10 M
- 23. NDV RT-PCR before treatment of AIV-NDV co infected samples with NDV antiserum NDV F gene (340 bp) NDV RT-PCR after treatment of AIV-NDV co infected samples with NDV antiserum M 1 2 3 4 5 6 7 8 9 10 11 12 M