MPHBS-2016-488-118
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This study investigated the relationship between MMP-9 gene expression and sialic acid (NANA) in human glial cell lines. Researchers treated glial cell lines with varying concentrations of NANA and found that MMP-9 mRNA expression increased with treatment, indicating NANA may be involved in signaling pathways regulating MMP-9 expression. The results suggest determining how NANA influences MMP-9 and other inflammatory molecules could provide insight into neurodegenerative diseases involving inflammation.
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Abstract(English)
Abstract(English)shahid chamran university of Ahvaz
油
This study investigated the relationship between MMP-9, TIMP-1, and sialic acid (NANA) in a human glial cell line and the effects of NANA on the expression of these genes. The study found that NANA upregulated the expression of both MMP-9 and TIMP-1 at lower concentrations in a way that maintained the MMP-9/TIMP-1 balance. However, at higher concentrations of 1000亮M NANA, MMP-9 expression was upregulated to a significantly greater degree than TIMP-1 expression, causing an imbalance similar to reports in neurodegenerative diseases. This suggests NANA may be involved in signaling pathways regulating the expression of these genes linked to neuroinflMS Poster
MS Postershahid chamran university of Ahvaz
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This study investigated the relationship between matrix metalloproteinase 9 (MMP-9), tissue inhibitor of metalloproteinase 1 (TIMP-1), and sialic acid (NANA) in human glial cells. Treatment with NANA upregulated MMP-9 and TIMP-1 mRNA expression, indicating NANA involvement in signaling pathways regulating these genes. At lower NANA concentrations, MMP-9 and TIMP-1 expression increased similarly, but at the highest concentration MMP-9 increased more than TIMP-1, resembling an imbalance seen in multiple sclerosis. This suggests NANA affects MMP-9 and TIMP-1 expression and their balance, which may influence inflammatory demyelination.A Characterization of the Effect of Methyltransferase Knockdown PDF
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This document discusses several mechanisms involved in gene expression and regulation at the molecular level. It describes how DNA is transcribed into messenger RNA, which then undergoes processing and modification before being translated into protein. Specifically, it mentions RNA methylation, transfer RNA, and epigenetic factors that can regulate gene expression without changing DNA sequence. The document also provides examples of how mutations in these molecular processes could lead to diseases or defective proteins if not properly regulated by the cell's quality control mechanisms.amgen poster
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ACE inhibitors like lisinopril, imidapril, and captopril were found to directly inhibit MMP-9 activity in addition to ACE activity. Studies in hamsters after myocardial infarction showed that lisinopril suppressed both ACE and MMP-9 activities, while an ARB did not affect MMP-9. Both lisinopril and imidapril significantly inhibited ACE and MMP-9 activities in rats after MI, with imidapril showing stronger MMP-9 inhibition. Captopril also dose-dependently inhibited ACE and MMP-9 activities in human plasma samples from MI patients. This suggests ACE inhibitors may provide benefits beyond blood pressure control by directly inhibiting MMP-9.Molecular mechanisms final
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Molecular Mechanisms in Urothelial Cancer Invasiveness: Systems Approach
This document discusses molecular pathways involved in the progression of urothelial carcinoma from non-invasive to invasive stages. It describes alterations in key pathways such as p53 and Rb that control cell cycle regulation and apoptosis. Proteases like MMPs degrade the extracellular matrix and promote angiogenesis, facilitating tumour invasion and metastasis. The interactions between pathways that regulate the extracellular environment, cell proliferation and cell death determine the invasiveness of urothelial carcinoma.Mission Mode Project (MMP)
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The extracellular matrix (ECM) is an organized network of extracellular materials that surrounds cells. One type of ECM is the basement membrane, a thin sheet that underlies epithelial tissues. The ECM is composed of collagen, elastin, proteoglycans, and structural glycoproteins. Collagen is the most abundant protein in the ECM and forms fibrils that provide structural support and determine the shape of tissues. Collagen molecules assemble into fibrils outside of cells through a process involving post-translational modifications.matrix metallo proteases (MMPs)
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Matrix metalloproteinases (MMPs) are a family of calcium-dependent zinc-containing endopeptidases that are responsible for tissue remodeling and degradation of the extracellular matrix. MMPs are excreted by connective tissue and inflammatory cells and play a role in both physiological and pathological processes such as angiogenesis. MMPs degrade various components of the extracellular matrix, including collagens, and their activation leads to tissue remodeling and degradation involved in conditions like cancer.project report on matrix cellular services
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Matrix Cellular Services has implemented a balanced scorecard to track performance across four perspectives: financial, customer, internal processes, and innovation/learning. The study found that organizational culture is conducive to implementation, but many employees are unsure of the objectives. While the company tracks targets well, not all departments use the balanced scorecard. It is suggested that Matrix improve communication of strategies and fully implement the balanced scorecard.Bmc Cancer Mmp3 2010
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This study investigated the association between the MMP-3 promoter polymorphism (-1171 5A->6A) and risk of oral submucous fibrosis (OSMF) and head and neck squamous cell carcinoma (HNSCC) in an Indian population. The authors genotyped 362 subjects, including 101 OSMF cases, 135 HNSCC cases, and 126 healthy controls. They found the 5A allele frequency was higher in OSMF (0.15) and HNSCC (0.13) compared to controls (0.07). Statistical analysis revealed a significant difference in 5A genotype frequency between OSMF and controls (p=0.01, OR=2.26) and between HNSCC4640-63316-1-PB
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- The document examines the role of plasminogen activator inhibitor 1 (PAI-1) in the recruitment of mast cells (MCs) to glioma tumors.
- It finds that neutralizing PAI-1 attenuates the infiltration of MCs into glioma tumors. It also finds that MCs express the PAI-1 receptor LRP1, and blocking LRP1 also attenuates MC migration.
- Activation of the potential PAI-1/LRP1 axis in MCs by purified PAI-1 promotes increased phosphorylation of STAT3 and subsequent MC exocytosis. This indicates the PAI-1/LRP1 axis influences MC recruitment in glioma tumors.Nucl. Acids Res.-2014-Di Lorenzo-8297-309
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This study identified protein arginine methyltransferase 6 (PRMT6) as a coactivator of nuclear factor-kappa B (NF-虜B) through three main findings:
1. Transgenic mice overexpressing a fusion of PRMT6 and the estrogen receptor displayed increased levels of interleukin 6 (IL-6), an NF-虜B target gene, upon tamoxifen treatment.
2. PRMT6 was found to directly interact with the NF-虜B subunit RelA and enhance the transcriptional activity of an NF-虜B reporter.
3. PRMT6 was recruited by RelA to selective NF-虜B target promoters upon TNF-留 stimulation and its overexpression led to increased nuclear accumulation of RelMisner Kauss Singh et al NAMPT cardiotox CardioVascular Tox 2016
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油
This document describes cardiotoxicity observed in rodents and in rat and human-derived cardiomyocyte cell lines treated with nicotinamide phosphoribosyltransferase inhibitors (NAMPTi). Short-term administration of several NAMPTis to rodents resulted in sudden death and signs of congestive heart failure. Further studies demonstrated that NAMPTi-induced toxicity in rat and human cardiomyocyte cell lines was on-target and likely human-relevant. Co-administration of nicotinic acid partially mitigated toxicity in vitro but not consistently in vivo. Human-derived cardiomyocyte assays were useful for assessing cardiotoxicity of compounds prior to in vivo studies.Oncotarget-2016
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油
The document summarizes research on using a small molecule mimetic of the Sin3A interaction domain (SID) decoy (C16) alone or in combination with retinoic acid receptor alpha (RAR留) agonists to treat triple negative breast cancer (TNBC). The key findings are:
1) C16 increases expression of RAR硫2 and endogenous retinoic acid levels, activating RAR target gene expression.
2) C16 combined with the RAR留 agonist AM80 strongly activates RARE-driven reporter gene expression more than either treatment alone.
3) In 3D culture, C16 combined with RAR留 agonists induces acinar morphogenesis and inhibits tumorsAntisense technologies and antisense oligonucleotides
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油
The document provides a comprehensive overview of antisense technologies and oligonucleotides, detailing their mechanism, characteristics, advantages, and limitations as a treatment for genetic disorders and infections. Antisense oligonucleotides work by binding to target mRNA to inhibit gene expression and have applications in various fields including oncology, cardiovascular, and inflammatory diseases. The text also discusses the challenges in their use, such as susceptibility to nucleolytic attack and the necessity for large doses.Dassie et al. Mol Ther 2014
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油
1) An RNA aptamer called A9g selectively inhibits the enzymatic activity of prostate-specific membrane antigen (PSMA) in prostate cancer cells.
2) Treatment of prostate cancer cells with A9g reduced cell migration and invasion in culture as well as metastatic disease in mouse models.
3) Pharmacokinetic and biodistribution studies in mice showed that A9g has target specificity for prostate cancer cells expressing PSMA and no significant off-target toxicity, supporting its potential as a novel targeted therapy for advanced prostate cancer.The Study of Routine Laboratory Factors in Children with Mycoplasma Pneumonia...
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(1) The study aimed to clarify the effects of serum uric acid (sUA) during Mycoplasma pneumoniae pneumonia (MPP) in children. (2) The results showed that sUA levels were higher in children with severe MPP compared to mild or no MPP. (3) The conclusion is that sUA has a protective anti-inflammatory effect during MPP in children. Further research is needed to understand the mechanisms of this effect.The Study of Routine Laboratory Factors in Children with Mycoplasma Pneumonia...
The Study of Routine Laboratory Factors in Children with Mycoplasma Pneumonia...HygeniaMirabel
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This study investigates the role of serum uric acid (SUA) in children with mycoplasma pneumonia, suggesting that SUA may have a protective anti-inflammatory effect during the illness. A cohort study analyzed various laboratory factors in children hospitalized with moderate to severe mycoplasma pneumonia, revealing that high SUA levels were associated with reduced inflammation. The findings indicate a need for further research to confirm the mechanisms behind SUA's protective role and its potential implications in human evolution.Antisense technologies and antisense oligonucleotides
Antisense technologies and antisense oligonucleotidesRangnathChikane
油
Antisense oligonucleotides are short strands of nucleic acids that bind to messenger RNA (mRNA) and inhibit gene expression. There are three main approaches for antisense oligonucleotides based on their ability to activate RNase H enzyme and resist nucleases. Antisense oligonucleotides show potential for treating various diseases like cancer, neurological disorders, and viral infections by hybridizing to target mRNA. However, challenges remain around protecting the oligonucleotides from degradation and improving cellular uptake and target specificity. Overall, antisense technology provides a novel approach for modulating gene expression and developing new therapies.Olaparib Upregulates miR-630 Expression to Enhance the Chemotherapeutic Sensi...
Olaparib Upregulates miR-630 Expression to Enhance the Chemotherapeutic Sensi...ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
油
This study explored the role of miR-630 in enhancing the chemotherapeutic sensitivity of BRCA1 mutant triple-negative breast cancer (TNBC) cell lines. The researchers found that combining carboplatin and gemcitabine chemotherapy with the PARP inhibitor olaparib upregulated miR-630 expression in BRCA1 mutant MDA-MB-436 and HCC1937 TNBC cell lines. Overexpression of miR-630 suppressed cell proliferation, migration, and invasion, whereas inhibition of miR-630 increased these effects. Therefore, miR-630 plays an important tumor suppressor role in increasing the chemotherapeutic sensitivity of PARP inhibitors for BRCA1 mutant TNBC, which may be one mechanism of how PAROkoye_NMDAR Inhibition Poster
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NMDA RECEPTOR INHIBITION REDUCES SURVIVAL OF MUTANT P53 BREAST CANCER CELL LINES
The study investigated the effects of inhibiting N-methyl D-aspartate receptors (NMDARs) in three mutant p53 breast cancer cell lines using the NMDAR antagonist MK-801. Treatment with MK-801 reduced cell viability in a dose-dependent manner and decreased levels of phosphorylated Akt, which mediates the anti-apoptotic effects of NMDAR activity. The results indicate that NMDAR activity increases cancer cell survival through an mTOR-dependent mechanism, and that NMDAR inhibition may be a potential less toxic treatment strategy for mutant pPancreatology - Molecular Biology - Isabella Pel%C3%A1ez C.pdf
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油
This document discusses the study of aurora kinase A inhibitor ml8237 as a potential treatment for pancreatic cancer, highlighting its ability to suppress cell growth and migration in pancreatic ductal adenocarcinoma (PDAC). The study employs various methods including transwell assays and immunohistochemistry to evaluate expression levels and efficacy in both cell lines and mouse models. Results suggest that the use of ml8237 may lead to improved outcomes in pancreatic cancer treatment, emphasizing the importance of molecular biology tools in understanding and addressing cancer development.Annals of Mutagenesis
Annals of Mutagenesis Austin Publishing Group
油
The document discusses the mutational and epimutational analyses of cell death-resistant tumor cells, focusing on uterine sarcomas and the roles of genetic and epigenetic factors in tumorigenesis and cancer therapy. Key findings indicate that mutations in the tumor suppressor p53 influence the mechanisms of apoptosis and autophagy, revealing potential therapeutic targets through the restoration of p53 function and epigenetic modifications. The research underscores the importance of distinguishing between driver and passenger mutations in cancer, emphasizing their implications for treatment approaches.5. ANTISENSE DRUG TECHNOLOGY-pharmacy.pptx
5. ANTISENSE DRUG TECHNOLOGY-pharmacy.pptxNooreHira16
油
Antisense drug technology focuses on the use of antisense oligonucleotides (ASOs) to inhibit gene expression at the mRNA level, preventing protein synthesis and potentially addressing genetic disorders. This technology has evolved over generations, with second and third-generation ASOs offering enhanced stability and reduced toxicity while effectively silencing target genes related to various diseases, including cancer and muscular dystrophy. Applications include gene therapy and targeted treatments for conditions like familial hypercholesterolemia and Duchenne muscular dystrophy, highlighting the therapeutic potential of these innovative approaches.Antisense therapy
Antisense therapyAshmita Chaudhuri
油
Antisense therapy uses synthetic nucleic acid strands that bind to messenger RNA (mRNA) to inactivate genes and their protein production. It has potential applications for treating various diseases like cancer, diabetes, and genetic disorders. Antisense oligonucleotides work by binding to mRNA, preventing translation into protein via RNase H activity or steric hindrance. While promising, antisense drugs require further clinical trials to prove efficacy compared to other treatments.More Related Content
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ACE inhibitors like lisinopril, imidapril, and captopril were found to directly inhibit MMP-9 activity in addition to ACE activity. Studies in hamsters after myocardial infarction showed that lisinopril suppressed both ACE and MMP-9 activities, while an ARB did not affect MMP-9. Both lisinopril and imidapril significantly inhibited ACE and MMP-9 activities in rats after MI, with imidapril showing stronger MMP-9 inhibition. Captopril also dose-dependently inhibited ACE and MMP-9 activities in human plasma samples from MI patients. This suggests ACE inhibitors may provide benefits beyond blood pressure control by directly inhibiting MMP-9.Molecular mechanisms final
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The extracellular matrix (ECM) is an organized network of extracellular materials that surrounds cells. One type of ECM is the basement membrane, a thin sheet that underlies epithelial tissues. The ECM is composed of collagen, elastin, proteoglycans, and structural glycoproteins. Collagen is the most abundant protein in the ECM and forms fibrils that provide structural support and determine the shape of tissues. Collagen molecules assemble into fibrils outside of cells through a process involving post-translational modifications.matrix metallo proteases (MMPs)
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Matrix metalloproteinases (MMPs) are a family of calcium-dependent zinc-containing endopeptidases that are responsible for tissue remodeling and degradation of the extracellular matrix. MMPs are excreted by connective tissue and inflammatory cells and play a role in both physiological and pathological processes such as angiogenesis. MMPs degrade various components of the extracellular matrix, including collagens, and their activation leads to tissue remodeling and degradation involved in conditions like cancer.project report on matrix cellular services
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油
Matrix Cellular Services has implemented a balanced scorecard to track performance across four perspectives: financial, customer, internal processes, and innovation/learning. The study found that organizational culture is conducive to implementation, but many employees are unsure of the objectives. While the company tracks targets well, not all departments use the balanced scorecard. It is suggested that Matrix improve communication of strategies and fully implement the balanced scorecard.Similar to MPHBS-2016-488-118 (20)
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This study investigated the association between the MMP-3 promoter polymorphism (-1171 5A->6A) and risk of oral submucous fibrosis (OSMF) and head and neck squamous cell carcinoma (HNSCC) in an Indian population. The authors genotyped 362 subjects, including 101 OSMF cases, 135 HNSCC cases, and 126 healthy controls. They found the 5A allele frequency was higher in OSMF (0.15) and HNSCC (0.13) compared to controls (0.07). Statistical analysis revealed a significant difference in 5A genotype frequency between OSMF and controls (p=0.01, OR=2.26) and between HNSCC4640-63316-1-PB
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- Activation of the potential PAI-1/LRP1 axis in MCs by purified PAI-1 promotes increased phosphorylation of STAT3 and subsequent MC exocytosis. This indicates the PAI-1/LRP1 axis influences MC recruitment in glioma tumors.Nucl. Acids Res.-2014-Di Lorenzo-8297-309
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The document summarizes research on using a small molecule mimetic of the Sin3A interaction domain (SID) decoy (C16) alone or in combination with retinoic acid receptor alpha (RAR留) agonists to treat triple negative breast cancer (TNBC). The key findings are:
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2) Treatment of prostate cancer cells with A9g reduced cell migration and invasion in culture as well as metastatic disease in mouse models.
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(1) The study aimed to clarify the effects of serum uric acid (sUA) during Mycoplasma pneumoniae pneumonia (MPP) in children. (2) The results showed that sUA levels were higher in children with severe MPP compared to mild or no MPP. (3) The conclusion is that sUA has a protective anti-inflammatory effect during MPP in children. Further research is needed to understand the mechanisms of this effect.The Study of Routine Laboratory Factors in Children with Mycoplasma Pneumonia...
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油
This study investigates the role of serum uric acid (SUA) in children with mycoplasma pneumonia, suggesting that SUA may have a protective anti-inflammatory effect during the illness. A cohort study analyzed various laboratory factors in children hospitalized with moderate to severe mycoplasma pneumonia, revealing that high SUA levels were associated with reduced inflammation. The findings indicate a need for further research to confirm the mechanisms behind SUA's protective role and its potential implications in human evolution.Antisense technologies and antisense oligonucleotides
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油
Antisense oligonucleotides are short strands of nucleic acids that bind to messenger RNA (mRNA) and inhibit gene expression. There are three main approaches for antisense oligonucleotides based on their ability to activate RNase H enzyme and resist nucleases. Antisense oligonucleotides show potential for treating various diseases like cancer, neurological disorders, and viral infections by hybridizing to target mRNA. However, challenges remain around protecting the oligonucleotides from degradation and improving cellular uptake and target specificity. Overall, antisense technology provides a novel approach for modulating gene expression and developing new therapies.Olaparib Upregulates miR-630 Expression to Enhance the Chemotherapeutic Sensi...
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The study investigated the effects of inhibiting N-methyl D-aspartate receptors (NMDARs) in three mutant p53 breast cancer cell lines using the NMDAR antagonist MK-801. Treatment with MK-801 reduced cell viability in a dose-dependent manner and decreased levels of phosphorylated Akt, which mediates the anti-apoptotic effects of NMDAR activity. The results indicate that NMDAR activity increases cancer cell survival through an mTOR-dependent mechanism, and that NMDAR inhibition may be a potential less toxic treatment strategy for mutant pPancreatology - Molecular Biology - Isabella Pel%C3%A1ez C.pdf
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This document discusses the study of aurora kinase A inhibitor ml8237 as a potential treatment for pancreatic cancer, highlighting its ability to suppress cell growth and migration in pancreatic ductal adenocarcinoma (PDAC). The study employs various methods including transwell assays and immunohistochemistry to evaluate expression levels and efficacy in both cell lines and mouse models. Results suggest that the use of ml8237 may lead to improved outcomes in pancreatic cancer treatment, emphasizing the importance of molecular biology tools in understanding and addressing cancer development.Annals of Mutagenesis
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油
The document discusses the mutational and epimutational analyses of cell death-resistant tumor cells, focusing on uterine sarcomas and the roles of genetic and epigenetic factors in tumorigenesis and cancer therapy. Key findings indicate that mutations in the tumor suppressor p53 influence the mechanisms of apoptosis and autophagy, revealing potential therapeutic targets through the restoration of p53 function and epigenetic modifications. The research underscores the importance of distinguishing between driver and passenger mutations in cancer, emphasizing their implications for treatment approaches.5. ANTISENSE DRUG TECHNOLOGY-pharmacy.pptx
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油
Antisense drug technology focuses on the use of antisense oligonucleotides (ASOs) to inhibit gene expression at the mRNA level, preventing protein synthesis and potentially addressing genetic disorders. This technology has evolved over generations, with second and third-generation ASOs offering enhanced stability and reduced toxicity while effectively silencing target genes related to various diseases, including cancer and muscular dystrophy. Applications include gene therapy and targeted treatments for conditions like familial hypercholesterolemia and Duchenne muscular dystrophy, highlighting the therapeutic potential of these innovative approaches.Antisense therapy
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油
Antisense therapy uses synthetic nucleic acid strands that bind to messenger RNA (mRNA) to inactivate genes and their protein production. It has potential applications for treating various diseases like cancer, diabetes, and genetic disorders. Antisense oligonucleotides work by binding to mRNA, preventing translation into protein via RNase H activity or steric hindrance. While promising, antisense drugs require further clinical trials to prove efficacy compared to other treatments.seminario biomol.pptx
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This document discusses a study on the role of the circular RNA hsa_circ_0000885 in osteosarcoma (OS). The study aimed to determine how hsa_circ_0000885 regulates OS. The results showed that silencing hsa_circ_0000885 suppressed OS progression and metastasis by regulating the expression of E2F3 and interacting with the miRNA miR-16-5p. The study provides evidence that hsa_circ_0000885 plays an oncogenic role in OS by modulating genes and miRNAs involved in proliferation and invasion.Nanolipoparticles-mediated MDR1 siRNA delivery: preparation, characterization...
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This document summarizes a study that prepared and characterized nanolipoparticles (NLPs) containing MDR1 siRNA for delivery to cancer cells. Key findings:
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This document summarizes a study that prepared and characterized nanolipoparticles (NLPs) containing MDR1 siRNA for delivery to cancer cells. NLPs were produced using the detergent dialysis method with PEGylated lipids. The resulting NLPs were 80-90 nm in size with a neutral surface charge and over 80% siRNA encapsulation efficiency. In vitro studies showed the NLPs had low cytotoxicity and improved cellular uptake of siRNA compared to a commercial transfection agent. The document concludes that NLPs containing MDR1 siRNA may be a good candidate for further in vivo siRNA delivery studies aimed at reversing multidrug resistance in cancer therapy.TRANSPARENT AI/ML TO DISCOVER NOVEL THERAPEUTICS FOR RNA SPLICING-MEDIATED DI...
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Nanolipoparticles-mediated MDR1 siRNA delivery: preparation, characterization...
Nanolipoparticles-mediated MDR1 siRNA delivery: preparation, characterization...Nanomedicine Journal (NMJ)
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CNVs have been found in many cancers including colon cancer and are thought to contribute to cancer development by altering gene dosage of oncogenes and tumor suppressors. Studies have shown that CNVs present in cancer cells are often absent in normal cells from the same patient. While the role of CNVs in cancer risk is still being explored, certain CNVs are associated with hereditary cancer syndromes that increase risk of colon cancer. Further research on cancer CNVs may lead to new biomarkers for cancer susceptibility, progression, and metastasis.RNA interference in Biology and medicine
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Schizophrenia and Geneticshahid chamran university of Ahvaz
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Cancer and CNVshahid chamran university of Ahvaz
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Pharmacogenomicsshahid chamran university of Ahvaz
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Pharmacogenomics is the study of how an individual's genetic inheritance affects their body's response to drugs. It involves studying the genetic basis for variability in drug efficacy and toxicity. The goal is to develop personalized medicine by understanding how genetic factors influence an individual's ability to metabolize and respond to drugs. Key factors that can vary between individuals include drug metabolizing enzymes, drug transporters, and drug targets. Genetic variations in these factors are associated with differences in drug efficacy or risk of adverse effects. Pharmacogenomic testing helps identify genetic polymorphisms that can predict drug response and dosing requirements.Ad
MPHBS-2016-488-118
- 1. *Corresponding Author: Hamid Galedari 118 1st International Conference on Medicine, Public Health and Biological Sciences (MPHBS) Study of MMP-9 gene expression in the presence of Sialic acid in human Glial cell line Narjes Khatoun Shabani Sadr1 , Mouhammad Shafiei1 , Hamid Galedari1,* , Alireza Khirolah2 1 Department of Genetic, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. 2 Department of Biochemistry, Medical School, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran. Abstract Introduction: Sialic acid (N-acetylneuraminic acid, NANA) is found at all cell surfaces of vertebrates which can cause many different reactions, such as starting or inhibiting the immune response, the activating of the complement system and cell signaling. Sialic acid involvement in the signaling pathways leading to MMP-9 expression in Glial cells is unclear. Therefore, this study investigated the relationship between MMP-9 and NANA and the effects of these ligand on the signaling processes of the inflammatory demyelination. Materials and methods: Human Glial cell line was prepared from Pasteur Institute of Iran and cultured in DMEM, supplemented with 10% FBS. The IC50 value of NANA was obtained by MTT assay. Glial cell line was treated with NANA (300,500 and 1000 袖g/ml) for 24h to investigate the effects of these ligand on the expression of MMP-9. Then total cellular RNA was isolated from approximately 6-5106 Glial cells. 1000 ng of total RNA from each sample was used into cDNA. Real-time PCR determined the expression level of the MMP-9 transcripts and REST and SPSS software were used for analyze. Results: The results of MTT assay were analyzed by Excel software and IC50 was obtained equivalent to 1273.3 袖M. Therefore, the concentration of Sialic acid was selected for treatments that were less than Ic50 (300,500 and 1000 袖M). By analyzing Real-time data, it was found that MMP-9 mRNA expression was up-regulated with treatment and indicated a possible involvement NANA on signaling pathway this gene expression. Conclusion: By determining the possible relationship between NANA and MMP-9 gene expression, which is one of a key inflammation factors in some neurodegenerative diseases, should be investigated MMPs gene control agents (including TIMPs and miRNAs); as well as other inflammatory macromolecules such as cytokines, chemokines, prostaglandins, NO and ROS. 息 2016 Published by CASRP publishing company Ltd. UK. Selection and/or peer-review under responsibility of Center of Advanced Scientific Research and Publications Ltd. UK. Keywords: Sialic acid (N-acetylneuraminic acid), NANA, MMP-9, Glial cell line, Multiple Sclerosis (MS). How to cite this article: Shabani Sadr, N.K., Shafiei, M., Galedari, H., Khirolah, A., 2016. Study of MMP-9 gene expression in the presence of Sialic acid in human Glial cell line. 1st International Conference on Medicine, Public Health and Biological Sciences (MPHBS), Sep. 2016. Access this article online Website: www.casrp.co.uk/conferences DOI: 10.18869/MPHBS.2016.118 Available online at http://www.casrp.co.uk/conferences CASRP Publisher