1) Nano-bubbles are smaller than microbubbles and have a longer lifetime, lasting several months compared to microbubbles which shrink and disappear within minutes. 2) Nano-bubbles are stabilized by a mechanism where ions accumulate on their surface, preventing the gas from dispersing and allowing the bubbles to persist for longer. 3) Ozone nano-bubbles are more effective at disinfecting and sterilizing than conventional ozone water methods, able to inactivate over 99% of viruses within 1 day.