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Fantastic Properties
of Nano-bubbles
Kaneo Chiba
Masayoshi Takahashi
Shrinking油油油
Disappear油
Microbubble油油油油 Nanobubble油油油油
Continue油
Ordinary油bubble油
(Macrobubble)
Goes油up油
rapidly
Burst油at油the油surface
鐚
Microbubble
Collapse
Physical stimulus
Nano-bubble production by collapsing microbubble
Radical generation
OH
Decomposition
of organic chemicals
Waste water treatment
Physical stimulus
Nanobubble production
Collapse
Microbubble
with salt (electrolytes)
Nano-bubble
OH
鐚
Nono-bubble generator
Microbubble
Nano-bubble
Physical stimulus
鐚
Analysis of nano-bubble by Dynamic light scattering (DLS)
0
2
4
6
8
10
12
14
16
18
20
76 93 113 138 168 204 249 304 370
Particle size (nm)
鐚
Microbubble 10 鐔30亮m
Nano-bubble
< 200nm
The deference of size between MB and NB
鐚
Shrinking
Disappear
Several minutes Several months
Microbubble Nanobubble
Continue
The deference of life time between MB and NB
Why ? 鐚
Movement of microbubbles in electrophoresis cell
Light
Microscope
Electrode
Electrophoresis cell
Water + microbubble
Electrode
( +   ) (  +)
鐚
time
龍potential鐚mV鐚
Change in 龍 potential of microbubble over time
Diameter 鐚亮m鐚
0 5 10 15 20 25 30
-20
-30
-40
-50
-60
-70
-80
Accumulation of surrounding ions
Ions Microbubble
time Ions
鐚鐚
with salt (electrolytes)
Aqueous solution
Nano-bubble
鐚
鐚 鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚
鐚 鐚
Stabilization mechanism of nanobubble (assumption)
Negatively charged
Surrounded by accumulated ions
(counter ions)
Prevention of gas dispersion
Salting out
鐚鐚
Nano-bubble generator
鐚鐚
Normal ozone water vs. Ozone nano-bubble
Ozone water ; disinfection & sterilizing 鐚Food industry, medical 鐚
1 hour
100 days
Bubbling
Micro-bubble Nano-bubble
0.1mg/L
1.5mg/L
1.5mg/L
More than
1.0mg/L
Ozone
Normal method
Nano-bubble
Collapse
13
Oyster
Norovirus
Norovirus symptoms:
vomiting, diarrhea,
a low-grade fever, chills,
headache, muscle aches
During
cultivation
How to treat the problem of norovirusHow to treat the problem of norovirus
14
Chlorine ( high concentration )
----- not good for foods
Chlorine
Norovirus
Conventional method
Oyster
Ozone water
Oyster
Ozone = strong oxidation ability

Virus  inactivation
Norovirus 15
Oysters in ozone nanobuble water for 1 day
16
Before ozone nanobubble treatment
17
More than 99% of feline calicivirus in the body --- inactivated
After ozone nanobubble treatment
18
Practical method for inactivation of norovirus by ozone nano-bubble
19
Carps : recovering from Carp herpes ( serious disease )
Active effect for fishes
20
Oxidation ability of ozone nano-bubble as a form of mist
21
more than 80% of original oxidation ability
reduction of smell and bacteria from atmospheres
22
Oxygen nanobubble
Collapse of oxygen microbubble About 1% salt
Recovery of weakened fishes
Active effect for creatures
23
Nano-Bubble Aquarium 24
Orchid in oxygen NB water
for more than 1 month
Astonishing effects on plant
Still alive
25
Tap water Oxygen nano-bubble water
White radish
Broccoli Broccoli
White radish
Initial growth of plants
26
Active effect for oysters
-20 for 1 day in oxygen NB
Room temperature
After 1 hour
Still alive
27
control TNF
alpha
With oxygen
nanobubble
control TNF
alpha
Prevention of arteriosclerosis (hardening of blood vessel )
Oxygen nanobubble inhibits mRNA expression induced by
TNF留 in RAOECs
ICAM1
VCAM1
GAPDH
Department of Cardiology,
Jichi Medical University
Application of nanobubble in medicine
Culture aorta endothelium of rat
Cytokine stimulation鐚TNF alpha鐚
Heart attack
Stroke
28
Vagus nerve of rat : after 1 week
Isotonic NaCl solution Oxygen nanobubble
Tokyo Medical and Dental University
Application of nanobubble in medicine
Preservation of living tissue / organ
29
30
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nano-bubble