This document provides instructions for exploring a pairwise ortholog matrix between genomes. It explains how to set the reference genome, ortholog detection method, and display options for the cells. Changing parameters requires clicking an apply button to update the table with the new settings.
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1. B I O I N F O R M A T I C S M A D E E A S Y / C h u n L a b , I n c . 1
Set the Reference
genome here. Orthologs are
displayed in the direction of
Reference Subject
genome. In this case,
Reference genome is the
second column.
How to explore pairwise
ortholog matrix
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2. B I O I N F O R M A T I C S M A D E E A S Y / C h u n L a b , I n c . 2
Set the Reference
genome here. Orthologs are
displayed in the direction of
Reference Subject
genome. In this case,
Reference genome is the
second column.
Set the Data Type or
algorithm for ortholog
detection. RBH (Reciprocal
Best Hit) is protein-based
whereas ORF-independent is
DNA-based.
How to explore pairwise
ortholog matrix
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3. B I O I N F O R M A T I C S M A D E E A S Y / C h u n L a b , I n c . 3
Set the display option in cells.
Contig# begin displays the
index (starting from 1) of
contigs of the genome and
their begin-positions. Bit
score ratio (%) displays how
similar the sequences are
between Reference and
Subject genomes.
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How to explore pairwise
ortholog matrix
4. B I O I N F O R M A T I C S M A D E E A S Y / C h u n L a b , I n c . 4
Set the display option in cells.
Contig# begin displays the
index (starting from 1) of
contigs of the genome and
their begin-positions. Bit
score ratio (%) displays how
similar the sequences are
between Reference and
Subject genomes.
Click [Apply] button for
applying parameters. The
content of table will remain
unchanged until this button is
clicked.
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How to explore pairwise
ortholog matrix