This study evaluated the safety attitudes of 290 operating room staff at two hospitals in Istanbul, Turkey. Researchers administered the Turkish version of the Safety Attitudes Questionnaire-Operating Room Version to assess reliability and validity. Results showed staff with over 11 years experience, those working only day shifts, volunteers, and those in rooms with 2-5 surgeries daily had higher safety attitude scores. Operating room staff reported higher team collaboration and communication with colleagues than other roles, though surgeons reported similar levels. The study recommends increasing education, resources, safety committees, training opportunities, and surveys to improve safety culture.