Plant biotechnology lab
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This document provides an overview of safety protocols for a plant biotechnology laboratory. It includes sections on general safety rules for using equipment like pipettes, gel electrophoresis, and pH meters. Hazards are outlined for specific reagents like BME and ethidium bromide. The document also discusses interests in the laboratory relating to climate change and food security issues in Bangladesh.
Plant biotechnology lab
- 1. Plant Biotechnology Lab Plants make people happy.
- 2. Table of Contents Introduction BMB Interest(PBT Lab) Lab Biosafety protocols 2 4/6/2022 Contents
- 3. Self-Introduction My name is Bidhan Sarkar , and I am a recent Biochemistry graduate from Dhaka University. In my free time ,I enjoy spending time with friends ,learning new things, reading and travelling. 3 4/6/2022 Add a footer
- 4. BMB Interest(PBT Lab) Climate Change- both the global warming caused by humans, and its impacts on Earth's weather patterns. Impact on Bangladesh Floods Droughts Sea level rise Prevention of Climate Change Reforstation Recycle 4 4/6/2022 Add a footer
- 5. Food Security -Both physical and economic access to sufficient food to meet dietary needs for a productive and healthy life. 5 4/6/2022 Food Security
- 6. Lab Guidelines Index General Safety Rules Reagents and commercial kits Working with pipettes, PCR tubes and tips Gel electrophoresis pH meter Nanodrop Working in Laminar 6 4/6/2022 Add a footer
- 7. General Safety Rules: 7 4/6/2022 Add a footer Safety Glasses Apron Gloves
- 8. Material Safety Data Sheet (MSDS) 8 4/6/2022 Add a footer
- 9. Laboratory Conduct Rules DO NOTTOUCH ANYTHING WITH WHICHYOU ARE NOT COMPLETELY FAMILIAR!!! NO SHEETS FROMTHE LAB PROTOCOL FILES CAN BE REMOVED. Autoclave time will be 10am in the morning and 3.30pm in afternoon 9 4/6/2022 Add a footer
- 10. Reagents and commercial kits BME can be toxic if ingested, and fatal if inhaled or absorbed through the skin. Vapors can irritate the eyes, mucous membranes, and respiratory tract. Use double mask when taking BME 10 4/6/2022 Add a footer
- 11. Working with pipettes, PCR tubes and tips: 11 4/6/2022 Add a footer Push the plunger down slowly to the point of first resistance: this sets the load volume. While holding the plunger at the load volume set point, put the tip into the solution so that it is immersed just enough to cover the end (3-4 mm). Slowly release the plunger to draw up the liquid making sure to keep the tip immersed. Visually inspect the load to make sure it is correct.
- 12. pH Meter & Nanodrop CalibratingYour pH Meter Place electrode in the buffer with a pH value of 7. Set the pH. Rinse your electrode with distilled water. Place your electrode in the appropriate buffer for your sample and begin reading. Open the NanoDrop software and select the NucleicAcid application. Use a small-volume, calibrated pipettor to perform a blank measurement by dispensing 1 亮L of buffer onto the lower optical surface. Lower the lever arm and select "Blank" in the NucleicAcid application 12 4/6/2022 Add a footer
- 13. Gel Electrophoresis Ethidium Bromide, commonly used to visualize nucleic acid, is a potent mutagen and should be handled with caution, even when mixed in the gel. Ethidium Bromide can be absorbed through the skin so it is also important to avoid any direct contact with the chemical. 13 4/6/2022 Add a footer
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