Plant dna extraction method
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This document discusses different methods of DNA isolation including the Maxwell 16 Plant DNA Kit method, spin column method, and CTAB method. It explains the structure of DNA and importance of DNA extraction for uses such as genetic testing, body identification, and analysis of forensic evidence. DNA can be stored long term at temperatures of -20属C, -80属C, -196属C or dried at room temperature. Isolated DNA has applications in understanding plant individuality, plant modification, forensic analysis, and solving historical puzzles.
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Introduction
The overall process
Uses of isolated genomic DNA
Extraction of DNA from plant material
Components of DNA extraction solutions
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Purification of DNA
CTAB Method
Phenolchloroform extraction
PROTEINASE K
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Magnetic beads
FTA Paper
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UV spectroscopy
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benefitsSecondary cell culture
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3) Purifying the DNA from other cell components like proteins and RNA. This is done using phenol-chloroform extraction and protease/RNase digestion. Ion-exchange chromatography can also be used.
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油
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- 4. DNA? Structure of DNA Method of DNA isolation Maxwell速 16 Plant DNA Kit Method Spin column Method CTAB Method Reagent Used In CTAB Method Procedure for CTAB method Why DNA Extraction? Storage of isolated DNA Use of the DNA
- 5. DNA? Something to say about DNA Cell Nucleus is the empire DNA
- 6. Structure of DNA Adenine-Thymine Base Pair Guanine-Cytosine Base Pair
- 7. 1. Maxwell速 16 Plant DNA Kit Method 2. Spin column Method 3. CTAB Method Method of DNA isolation
- 8. Ridge end Label end Contents Lysis Buffer MagneSil PMPs Wash Buffer Wash Buffer Wash Buffer Wash Buffer Wash Buffer Plunger Reagent Cartridge Maxwell速 16 Plant DNA Kit Method An easy method efficient Automated purification of genomic DNA from plant tissue samples.
- 9. Spin column Method It is a solid phase extraction method Quickly purify nucleic acids. Nucleic acid will bind to the solid phase of silica under certain conditions.
- 10. CTAB Method Detergent+ Lipid & Proteins Detergent DNA CTAB Stands for cetyl trimethyl ammonium bromide
- 11. oCTAB oChloroform oIsoamyl alchohol oIsopropyl alcohol oRNAse oQiagen oAmbion o Proteinase K oNaCl oMercaptoethanol oAmmonium acetate
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- 13. Why DNA Extraction? Genetic Testing Body Identification Analysis of Forensic Evidence
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- 15. Understanding Individuality of Plants Using genetic information of DNA for plant modification Use in forensic analysis Use to solve historical puzzle