Plant dna extraction method
- 4. • DNA? • Structure of DNA • Method of DNA isolation • Maxwell® 16 Plant DNA Kit Method • Spin column Method • CTAB Method • Reagent Used In CTAB Method • Procedure for CTAB method • Why DNA Extraction? • Storage of isolated DNA • Use of the DNA
- 5. DNA? Something to say about DNA Cell Nucleus is the empire DNA
- 6. Structure of DNA Adenine-Thymine Base Pair Guanine-Cytosine Base Pair
- 7. 1. Maxwell® 16 Plant DNA Kit Method 2. Spin column Method 3. CTAB Method Method of DNA isolation
- 8. Ridge end Label end Contents Lysis Buffer MagneSil PMPs Wash Buffer Wash Buffer Wash Buffer Wash Buffer Wash Buffer Plunger Reagent Cartridge Maxwell® 16 Plant DNA Kit Method An easy method efficient Automated purification of genomic DNA from plant tissue samples.
- 9. Spin column Method It is a solid phase extraction method Quickly purify nucleic acids. Nucleic acid will bind to the solid phase of silica under certain conditions.
- 10. CTAB Method Detergent+ Lipid & Proteins Detergent DNA CTAB Stands for cetyl trimethyl ammonium bromide
- 11. oCTAB oChloroform oIsoamyl alchohol oIsopropyl alcohol oRNAse oQiagen oAmbion o Proteinase K oNaCl oMercaptoethanol oAmmonium acetate
- 12. Procedure for CTAB method Sample extract CATB Centrifuge Carbohydrates, proteins, etc. Nucleic acid- CTAB complex DNA Precipitated Nucleic acid-CTAB complex
- 13. Why DNA Extraction? • Genetic Testing Body Identification • Analysis of Forensic Evidence
- 14. There are four temperature based strategies for long term DNA conservation. These are storage at: -20° C -80° C -196° C Dried, at room temperature Store DNA in dry state, reduces molecular mobility and inhibits the depurination, depyrimidination, deamination and hydrolytic The DNA immobilized on FDA cards is amenable to PCR after 17 years storage. DNA in aqueous phase is stored under slightly basic conditions. At -196° C, DNA is maintained in a vitreous state General consideration for DNA storage:
- 15. Understanding Individuality of Plants Using genetic information of DNA for plant modification Use in forensic analysis Use to solve historical puzzle