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References:
1. SousaCA, ClairouinM,Seixas G, ViveirosB, NovoMT,et al. (2012) Ongoingoutbreakof denguetype 1 in the AutonomousRegion ofMadeira,Portugal:preliminaryreport.EuroSurveill17: 811
2. Seixas G (2012) Aedes (Stegomyia)aegypti (Diptera,Culicidae)from MadeiraIsland:geographicaloriginandinsecticide resistanceUniversidadeNova deLisboa.
3. NazarethT, Teod坦sio R,Porto G, Gon巽alvesL, Seixas G, et al. (2014) Strengtheningtheperception-assessmenttoolsfor dengueprevention:a cross-sectionalsurveyin a temperate region (Madeira, Portugal).BMCPublicHealth14: 39.
Acknowledgements:
ProjectoFCT- Dengueno Arquip辿lagodaMadeira- Ref: PTDC/SAL-EPI/115853/2009
GABBA FCT Bolsa de doutoramento Ref : SFRH / BD / 51012 / 2010
Planning dengue-preventive campaigns:
Aedes aegyptis breeding sites and community perceptions in Madeira Island
futuros surtos.
p < 0.001 (Mann-Whitney test)
I -
- Flower pot dish (52.7%)
- Out-door sinks (35.7%)
- Floor water accumulation (23.3%)
- Flower jar (21.7%)
- Pet water dish (18.8%)
- Flower pot/ bucket (14.1%)
- Rubbin bish (uncovered) (10.3%)
- Rain gutters (6.9%)
- Other material (toys, ashtrays, etc.) (6.8%)
- Water flower (5.7 %)
- Rubbish (on th floor) (5.4%)
- Toleit pan (not used often) (2.3%)
- Water tank/well (2.3%)
- Fridge trays (2.2%)
- Lake/cascade (1.9%)
- Drum/container (1.2%)
- Building material (1.2%)
- Rubble (1.1%)
- Swiming pool (untreated and uncovered) (0.5%)
- Tyres (0.5%)
- Pieces of glass on wall (0.3 %)
Flower-pot dishes were the most frequent Ae.
aegyptis breeding site. Results suggest an
atypical infestation pattern consisting of
d辿cor/leisure containers (rather than the
commonly reported waste-disposal and water-
supply ones).
This work constitutes the first entomological
report in European countries.
3.1.
2.
4.
5.
Two cross-sectional surveys were performed (A): one entomological to describe the presence / type of breeding sites in communitys
households, and one epidemiological, to assess individuals perceptions regarding the elimination of breeding sites. The latter was evaluated
through a score-based tool (B) which measures the level of understanding of this proposed behavior [3].
1GABBA Doctoral Program, ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Porto, Portugal; 2GHTM-Global Health and Tropical Medicine; 3Unidade Cl鱈nica Tropical, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 4Basic & Clinical Research on Iron
Biology, IBMC, Institute for Molecular and Cellular Biology, Porto, Portugal; 5Unidade de Sa炭de P炭blica e Internacional e Bioestat鱈stica, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 6Centro de Estat鱈stica e Aplica巽探es da Universidade de Lisboa (CEAUL), Lisboa, Portugal; 7Unidade
de Parasitologia M辿dica, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 8Departamento de Sa炭de, Planeamento e Administra巽達o Geral, Instituto de Administra巽達o da Sa炭de e Assuntos Sociais, Madeira, Portugal;
Nazareth T1,2,, Teod坦sio R2,3, Porto G1,4, Gon巽alves L2,5,6, Novo MT2,7, Seixas G2,7, Silva AC8, Sousa CA2,7
Concept 1 - Knew that mosquitoes can transmit diseases
Concept 2 - Presented a correct example of a mosquito-borne disease
Concept 3 - Recognized the presence of mosquitoes that can transmit disease
Concept 4 - Considered the high-possibility of dengue emergence in Madeira
Concept 5 - Knew that mosquitoes can breed inside houses
Concept 6 - Considered the relevance of the vector-control in the domestic area
Concept 7 - Recognized water-containers as a mosquito breeding contributor
Concept 8  Did not recognize false* mosquito breeding contributors
Concept 9 - Recognized breeding-site removal as a effective measure
Concept 10  Did not recognize erroneous practices as effective 
Households WITHOUT breeding sites
Residents reveal an EP-score > 7
Households WITH breeding sites
Residents reveal an EP-score < 7
A  Entomological and epidemiological cross-sectional survey B  Essential Perception Score (EP-score, 0-10 points)
C - List of prospected breeding-sites
and respective prevalence
D  Scheme of the TOP 5 most frequent
breeding sites
E  Association between communitys
perceptions and practices
RESULTS
BACKGROUND
There is still no vaccine nor anti-viral treatment available, hence vector control strategies remain key for
dengue prevention/control. Given the reported high-resistance levels to critical insecticides of the local Ae.
aegyptis population [2], the promotion of source-reduction activities based on the elimination of larvae
habitats/breeding sites is, thus, mandatory as the unique preventive strategy currently available.
Moreover, by assessing community perceptions regarding source
reduction activities and simultaneously checking the presence of
breeding sites in their households, it aims to compare individuals
Since Ae. aegyptis breeding sites are commonly water containers placed inside or around human households, the
community participation is decisive in its elimination.
With the arrival of Aedes aegypti into Madeira
Territory, and mainly after its first dengue outbreak in 2012 [1], the planning of preventing campaigns is a
priority for local and European public health.
perceptions and respective source
bbbbreduction practices.
This study aims to describe the most frequent Ae. aegypti breeding sites in Madeiras infested
areas, in order to prioritize which water containers must be preferably eliminated.
METHODS CONCLUSIONS
Findings will not only guide the planning of
target-oriented local campaigns, but also will
support a global reflection on dengue
prevention in urbanized regions.
Finally, present entomological survey
enabled the validation of the EP-score as
a tool to assess community perceptions
regarding breeding site elimination.
A total of 1276 households/residents participated in the study. Participants and their houses were randomly out of an universe of 13 433 adult
residents in the infested area, fulfilling the objectives of this study (90% confidence level and 2.5% precision error).
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Poster EMCA 2015 FINAL_TN

  • 1. References: 1. SousaCA, ClairouinM,Seixas G, ViveirosB, NovoMT,et al. (2012) Ongoingoutbreakof denguetype 1 in the AutonomousRegion ofMadeira,Portugal:preliminaryreport.EuroSurveill17: 811 2. Seixas G (2012) Aedes (Stegomyia)aegypti (Diptera,Culicidae)from MadeiraIsland:geographicaloriginandinsecticide resistanceUniversidadeNova deLisboa. 3. NazarethT, Teod坦sio R,Porto G, Gon巽alvesL, Seixas G, et al. (2014) Strengtheningtheperception-assessmenttoolsfor dengueprevention:a cross-sectionalsurveyin a temperate region (Madeira, Portugal).BMCPublicHealth14: 39. Acknowledgements: ProjectoFCT- Dengueno Arquip辿lagodaMadeira- Ref: PTDC/SAL-EPI/115853/2009 GABBA FCT Bolsa de doutoramento Ref : SFRH / BD / 51012 / 2010 Planning dengue-preventive campaigns: Aedes aegyptis breeding sites and community perceptions in Madeira Island futuros surtos. p < 0.001 (Mann-Whitney test) I - - Flower pot dish (52.7%) - Out-door sinks (35.7%) - Floor water accumulation (23.3%) - Flower jar (21.7%) - Pet water dish (18.8%) - Flower pot/ bucket (14.1%) - Rubbin bish (uncovered) (10.3%) - Rain gutters (6.9%) - Other material (toys, ashtrays, etc.) (6.8%) - Water flower (5.7 %) - Rubbish (on th floor) (5.4%) - Toleit pan (not used often) (2.3%) - Water tank/well (2.3%) - Fridge trays (2.2%) - Lake/cascade (1.9%) - Drum/container (1.2%) - Building material (1.2%) - Rubble (1.1%) - Swiming pool (untreated and uncovered) (0.5%) - Tyres (0.5%) - Pieces of glass on wall (0.3 %) Flower-pot dishes were the most frequent Ae. aegyptis breeding site. Results suggest an atypical infestation pattern consisting of d辿cor/leisure containers (rather than the commonly reported waste-disposal and water- supply ones). This work constitutes the first entomological report in European countries. 3.1. 2. 4. 5. Two cross-sectional surveys were performed (A): one entomological to describe the presence / type of breeding sites in communitys households, and one epidemiological, to assess individuals perceptions regarding the elimination of breeding sites. The latter was evaluated through a score-based tool (B) which measures the level of understanding of this proposed behavior [3]. 1GABBA Doctoral Program, ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Porto, Portugal; 2GHTM-Global Health and Tropical Medicine; 3Unidade Cl鱈nica Tropical, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 4Basic & Clinical Research on Iron Biology, IBMC, Institute for Molecular and Cellular Biology, Porto, Portugal; 5Unidade de Sa炭de P炭blica e Internacional e Bioestat鱈stica, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 6Centro de Estat鱈stica e Aplica巽探es da Universidade de Lisboa (CEAUL), Lisboa, Portugal; 7Unidade de Parasitologia M辿dica, Instituto de Higiene e Medicina Tropical, Lisboa, Portugal; 8Departamento de Sa炭de, Planeamento e Administra巽達o Geral, Instituto de Administra巽達o da Sa炭de e Assuntos Sociais, Madeira, Portugal; Nazareth T1,2,, Teod坦sio R2,3, Porto G1,4, Gon巽alves L2,5,6, Novo MT2,7, Seixas G2,7, Silva AC8, Sousa CA2,7 Concept 1 - Knew that mosquitoes can transmit diseases Concept 2 - Presented a correct example of a mosquito-borne disease Concept 3 - Recognized the presence of mosquitoes that can transmit disease Concept 4 - Considered the high-possibility of dengue emergence in Madeira Concept 5 - Knew that mosquitoes can breed inside houses Concept 6 - Considered the relevance of the vector-control in the domestic area Concept 7 - Recognized water-containers as a mosquito breeding contributor Concept 8 Did not recognize false* mosquito breeding contributors Concept 9 - Recognized breeding-site removal as a effective measure Concept 10 Did not recognize erroneous practices as effective Households WITHOUT breeding sites Residents reveal an EP-score > 7 Households WITH breeding sites Residents reveal an EP-score < 7 A Entomological and epidemiological cross-sectional survey B Essential Perception Score (EP-score, 0-10 points) C - List of prospected breeding-sites and respective prevalence D Scheme of the TOP 5 most frequent breeding sites E Association between communitys perceptions and practices RESULTS BACKGROUND There is still no vaccine nor anti-viral treatment available, hence vector control strategies remain key for dengue prevention/control. Given the reported high-resistance levels to critical insecticides of the local Ae. aegyptis population [2], the promotion of source-reduction activities based on the elimination of larvae habitats/breeding sites is, thus, mandatory as the unique preventive strategy currently available. Moreover, by assessing community perceptions regarding source reduction activities and simultaneously checking the presence of breeding sites in their households, it aims to compare individuals Since Ae. aegyptis breeding sites are commonly water containers placed inside or around human households, the community participation is decisive in its elimination. With the arrival of Aedes aegypti into Madeira Territory, and mainly after its first dengue outbreak in 2012 [1], the planning of preventing campaigns is a priority for local and European public health. perceptions and respective source bbbbreduction practices. This study aims to describe the most frequent Ae. aegypti breeding sites in Madeiras infested areas, in order to prioritize which water containers must be preferably eliminated. METHODS CONCLUSIONS Findings will not only guide the planning of target-oriented local campaigns, but also will support a global reflection on dengue prevention in urbanized regions. Finally, present entomological survey enabled the validation of the EP-score as a tool to assess community perceptions regarding breeding site elimination. A total of 1276 households/residents participated in the study. Participants and their houses were randomly out of an universe of 13 433 adult residents in the infested area, fulfilling the objectives of this study (90% confidence level and 2.5% precision error).