Ppt of centrifugation
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Centrifugation is a process that uses centrifugal force to separate mixtures based on density differences. It works by spinning samples at high speeds, causing denser particles to sediment out of solution. There are two main types - preparative centrifugation which separates large amounts of samples, and analytical centrifugation which analyzes small amounts. Centrifugation has many applications including isolating suspensions, isotope separation, and separating cellular components.
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Centrifugation
CentrifugationSabahat Ali
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Centrifugation is a technique that uses centrifugal force to separate mixtures based on density. It works by spinning samples at high speeds, which causes heavier components to sediment. There are several types including preparative centrifugation to separate/purify biological samples, analytical centrifugation to determine physical characteristics, differential centrifugation to separate cell components, density gradient centrifugation to separate mixtures based on density differences, and ultracentrifugation using very high speeds. Centrifugation has many applications in research, medicine, and industry.Centrifugation
CentrifugationTapeshwar Yadav
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Centrifugation is the separation technique commonly used in clinical and research laboratories.
It is based on the behavior of particles in an applied centrifugal field.
More dense components of the mixture move away from the axis of the centrifuge while less dense components of the mixture move towards the axis.
Centrifugation principle and types by Dr. Anurag Yadav
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concept of cnetrifugation,
basic Principle
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application of the each type of centrifuge
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CentrifugationShri Shankaracharya College, Bhilai,Junwani
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Centrifugation is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures to separate the two miscible substances ,and also to analyze the hydrodynamic properties of macro molecule with a centrifuge , used in industry and in laboratory setting.
Analytical Ultracentrifugation
Analytical UltracentrifugationAkumpaul
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Includes concepts of Centrifugation; Types of centrifugations; Analytical Ultracentrifugation in detail; Marker enzymes overviewUltra centrifuge
Ultra centrifugeSuganyaPaulraj
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Ultracentrifugation is a specialized technique that spins samples at extremely high speeds, up to 150,000 rpm, allowing separation of smaller particles like viruses and proteins. It operates in a vacuum chamber to eliminate air friction and attain high speeds with little energy. Modern ultracentrifuges have optical devices to observe and photograph sedimentation and are used to separate organelles, membranes, and purify proteins and nucleic acids.centrifuge principle and application
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Centrifuge centrifugal force centrifugal working of centrifuge type of centrifuge RPM rotation pre minutes rcf MLT laboratory Differential centrifugation
Differential centrifugationSuganyaPaulraj
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Differential centrifugation is a technique used to separate cellular components like organelles based on sedimentation rate differences caused by varying density, size, shape when spun at increasing centrifugal forces; it involves homogenizing a sample, centrifuging sequentially at low speeds to pellet larger components then higher speeds to further separate smaller ones, allowing fractionation of components from nuclei to ribosomes into pellets and supernatants. Differential centrifugation has applications in separating various mixtures and purifying biomolecules, cells, and subcellular structures.Centrifugation
CentrifugationRachana Choudhary
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Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The larger the size and the larger the density of the particles, the faster they separate from the mixture.Principles and applications of centrifugation ppt
Principles and applications of centrifugation pptpoojakamble1609
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This document discusses the principles and applications of centrifugation. It defines centrifugation as using centripetal force to separate substances of different densities. There are three main types of centrifuges: low-speed centrifuges which operate at speeds up to 5000rpm; high-speed centrifuges which allow more control over speed and temperature; and ultracentrifuges, the most sophisticated, which operate at very high speeds and require vacuum and temperature control. The main applications of centrifugation are preparative techniques like sedimentation and differential centrifugation, and analytical techniques like density gradient and zonal centrifugation which are used to separate and analyze viruses, organelles, and other particles.centrifugation
centrifugationSanjay Sinhmar
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Centrifugation uses centrifugal force to separate particles in a solution based on properties like size, shape, density. There are several types of centrifugation including density gradient centrifugation, differential centrifugation, and ultracentrifugation. Density gradient centrifugation separates particles based on buoyant density into zones, while differential centrifugation separates organelles from cells. Ultracentrifugation uses very high speeds and forces particle separation. Centrifugation has many applications in areas like water treatment, biomedical research, and industries like sugar and oil production.Centrifugation
Centrifugationfarzeen javaid
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The document discusses centrifuges and centrifugation. It begins by summarizing the early history of centrifuges, including inventions in the 18th and 19th centuries. It then provides definitions and explanations of key terms like centrifuge, centrifugation, and relative centrifugal force. The rest of the document details different types of centrifuges, components of centrifuges, separation techniques, and rotors and tubes used in centrifugation.Centrifugation techniques
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1. Instrumental analysis involves using instruments to convert physical or chemical characteristics of analytes into interpretable information. This includes clinical chemistry which aims to facilitate accurate diagnostic testing.
2. Centrifugation techniques use high centrifugal force to separate particles or molecules in a suspension more quickly than gravity alone. This involves spinning samples at high revolutions per minute in different types of centrifuges.
3. Centrifuges vary in size from microcentrifuges suitable for small volumes to preparative or analytical ultracentrifuges capable of very high speeds. The type used depends on the separation or analysis needed. All aim to control variables like speed, radius, and temperature to achieve reliable separations.Centrifugation notes slideshare 
Centrifugation notes slideshare strawverrymilktae
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Centrifugation is a technique that uses centrifugal force to separate components of a solution based on properties like density, size, and shape. It works by spinning the solution at high speeds, allowing heavier components to sediment to the bottom. There are two main types - density gradient centrifugation, where particles separate through a density gradient, and differential centrifugation, which separates organelles from cells. Centrifugation has many applications, including separating cream from milk, clarifying wine, and purifying proteins and cells.Centrifugation
CentrifugationOyshe Ahmed
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Centrifugation is a process that uses centrifugal force to separate particles or molecules based on their size, shape, or density. It involves spinning a sample in a centrifuge to separate it into its components. There are various types of centrifugation classified based on speed, temperature, or separation method including differential, isopycnic, sucrose gradient, and ultracentrifugation. Centrifugation has many applications in industries like pharmaceuticals, water treatment, and oil extraction as well as in research areas like biochemistry and molecular biology.Chromatography and Its Types
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Chromatography is a technique used to separate and identify components of a mixture. It works by distributing molecules between a stationary and mobile phase. Molecules that spend more time in the mobile phase move faster through the column. There are several types of chromatography classified by mobile phase or separation mechanism, including gas chromatography which uses gases, thin layer chromatography which uses adsorbents on plates, and liquid chromatography which uses liquids. Chromatography is used in various applications such as pharmaceutical analysis, environmental monitoring, and forensic analysis.Centrifugation
CentrifugationDN Vaisnavi
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This document discusses centrifugation and provides details about its history, basic principles, types of centrifuges and rotors, and applications. Centrifugation uses centrifugal force to separate mixtures based on density. It has been used since the 1400s and important developments include the first analytical ultracentrifuge in 1920. Common centrifuges include low-speed, high-speed, and ultracentrifuges, which separate particles based on mass and density. Rotors include swinging bucket, fixed angle, and vertical designs. Centrifugation has many clinical and laboratory applications, including separating blood components and subcellular particles.Electrophoresis and its application
Electrophoresis and its applicationDIPANKAR SAIKIA
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Electrophoresis:
Electrophoresis is separation technique based on movement of charge particle in an electric field.
Movement of charge particles can be determined by following formula--
V= Eq/f
Where,
V= Velocity of the charged particle;
E= electric field of the molecule;
q= Net charge of the molecule; and
f= Frictional co-efficient of the molecule
Types of electrophoresis:
1. Agarose gel electrophoresis ;
2. Poly-acryl amide gel electrophoresis [PAGE];
3. Sodium do-decyl sulphate Poly- acrylamide gel electrophoresis [SDS-PAGE] ;
4. Two dimensional Poly-acrylamide gel electrophoresis [2D-PAGE];
5. Pulse field gel electrophoresis [PFGE];
6. Capillary gel electrophoresis [CGE]; and
7. Disc electrophoresis for Protein.
Application of electrophoresis:
1. Estimation of the DNA molecule.[ Agarose , PAGE ]
2. Analysis of PCR product. [ Agarose ]
3. Separation of restricted genomic DNA and RNA. [Agarose and PAGE respectively]
4. Conformation of newly isolated DNA .[Agarose]
5. Separation of most small fragments of DNA. [PAGE]
6. In forensic science.[Agarose , PAGE, SDS-PAGE, 2D PAGE ,Capillary gel electrophoresis , PFGE]
8. In determining molecular wt. of protein.[SDS-PAGE].etc
Types of centrifuges
Types of centrifugesShilpa Bhat
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The document describes different types of centrifuges based on their design features and intended applications. Centrifuges vary in maximum speed, capacity, temperature control, and sample volume capabilities. Small benchtop centrifuges are used in clinical labs for blood separation and can hold around 100 tubes. Microcentrifuges are very common in biology labs, can hold small tube volumes, and generate forces up to 15,000g. High speed centrifuges spin at 15,000-20,000 RPM and are used for research applications requiring separation of cellular components. Ultracentrifuges provide the highest speeds and forces but are expensive and require special rotors and cooling due to heat generation.Polyacrylamide gel electrophoresis
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PAGE is a subtype of the gel electrophoresis whereby the normal gel is replaced with polyacrylamide gels use as the support matrix.
widely used and has very much importance.
COMPLETE PROCEDURE & USES are described in the slide.
Phase contrast & Flurosence microscope
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This document provides an introduction to phase contrast microscopy and fluorescent microscopy. It discusses that phase contrast microscopy, developed in 1934, uses optical techniques to produce high-contrast images of transparent samples like living cells. It works by converting differences in refractive index to intensity differences visible to the eye. Fluorescent microscopy illuminates samples with high-energy light, causing fluorophores to emit lower-energy light, which can then be filtered and observed. Both techniques allow viewing of unstained, living samples like cells in more detail.Ultracentrifugation 
Ultracentrifugation Aswathi K S
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The document discusses ultracentrifugation, which uses high centrifugal forces to separate particles in solutions based on size, shape, and density. It describes:
1) How particles experience centrifugal, buoyant, and frictional forces when spun in an ultracentrifuge.
2) Key terms like sedimentation rate, sedimentation coefficient, and angular velocity.
3) Types of ultracentrifugation experiments like sedimentation velocity and equilibrium experiments.
4) Types of preparative ultracentrifugation like differential, density gradient, zonal, and isopycnic centrifugation used to separate cell components.
5) Components of an ultracentrifuge like rotors, buckets,Centrifugation (Extraction Technique)
Centrifugation (Extraction Technique)Zoologist Pakistan
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際際滷s about Centrifugation
際際滷s include Definition, History, Basic Principle, Instrumentation, Low Speed Centrifuges, High Speed Centrifuges, Ultra centrifuges, Types of Rotor, Preparative Techniques, Analytical Techniques, Applications of Centrifugation & Significance of centrifugationPAGE- Electrophoresis
PAGE- ElectrophoresisTapeshwar Yadav
油
It is a subtype of the gel electrophoresis whereby the normal gel is replaced with polyacrylamide gels used as support media.
Gels are made by free radical-induced polymerization of acrylamide and N,N-Methylenebisacrylamide.
It is the most widely used technique of electrophoresis.
Principles and application of chromatography
Principles and application of chromatographysuniu
油
Chromatography is a laboratory technique used to separate mixtures by distributing components to separate between two phases, one stationary and one mobile. It works based on how substances partition between the phases and move through the system at different rates. Common techniques include column chromatography, thin layer chromatography, gas chromatography, and high performance liquid chromatography. Chromatography is used in analytical chemistry to identify unknown substances and quantify components in a mixture.PHASE MICROSCOPY
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This document discusses phase contrast microscopy. It begins by defining a microscope and microscopy. It then describes the main types of microscopes, including optical, electron, and scanning probe microscopes. It focuses on the light microscope, explaining that it uses visible light and is commonly used in biology to view structures like cells. It defines magnification and resolution. It describes the different modes of light microscopy - bright field, dark field, and phase contrast - and explains that phase contrast microscopy allows observation of living cells by enhancing contrast between structures with small refractive index differences. It notes that phase contrast microscopy was invented by Frits Zernike, for which he won the Nobel Prize in Physics in 1953.Centrifugation 
Centrifugation Sajad Rather
油
Centrifugation is a procedure that uses centrifugal force to separate mixtures. Denser components move away from the axis of rotation while less dense components move towards the axis. The document discusses the principles, types (low speed, high speed, ultracentrifuges), applications, and techniques (preparative, differential, density gradient) of centrifugation. It provides details on rotor types, speeds, uses for separating organelles, macromolecules, and more. Diagrams illustrate basic centrifuge components and a table compares characteristics of different centrifuge types.Centrifugation techniques by kk sahu
Centrifugation techniques by kk sahuKAUSHAL SAHU
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Introduction
Definition
Components of centrifuge
Types of centrifugation
Application
Conclusion
References
A centrifugation is a technique for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed.
Centrifugation
CentrifugationBibatsu
油
Centrifugation is a process that uses centrifugal force to separate mixtures of particles based on density. It works by spinning a sample rapidly, causing denser particles to migrate outward while less dense particles move inward. There are various types of centrifuges and rotors that can be used for different applications like separating blood components, purifying proteins, or clarifying wine. Common techniques include differential centrifugation to separate cell components, density gradient centrifugation to separate mixtures based on buoyant density, and ultracentrifugation for high-speed separations. Centrifugation is widely used in industries like water treatment and laboratories for analytical purposes.More Related Content
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Differential centrifugation is a technique used to separate cellular components like organelles based on sedimentation rate differences caused by varying density, size, shape when spun at increasing centrifugal forces; it involves homogenizing a sample, centrifuging sequentially at low speeds to pellet larger components then higher speeds to further separate smaller ones, allowing fractionation of components from nuclei to ribosomes into pellets and supernatants. Differential centrifugation has applications in separating various mixtures and purifying biomolecules, cells, and subcellular structures.Centrifugation
CentrifugationRachana Choudhary
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Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The larger the size and the larger the density of the particles, the faster they separate from the mixture.Principles and applications of centrifugation ppt
Principles and applications of centrifugation pptpoojakamble1609
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This document discusses the principles and applications of centrifugation. It defines centrifugation as using centripetal force to separate substances of different densities. There are three main types of centrifuges: low-speed centrifuges which operate at speeds up to 5000rpm; high-speed centrifuges which allow more control over speed and temperature; and ultracentrifuges, the most sophisticated, which operate at very high speeds and require vacuum and temperature control. The main applications of centrifugation are preparative techniques like sedimentation and differential centrifugation, and analytical techniques like density gradient and zonal centrifugation which are used to separate and analyze viruses, organelles, and other particles.centrifugation
centrifugationSanjay Sinhmar
油
Centrifugation uses centrifugal force to separate particles in a solution based on properties like size, shape, density. There are several types of centrifugation including density gradient centrifugation, differential centrifugation, and ultracentrifugation. Density gradient centrifugation separates particles based on buoyant density into zones, while differential centrifugation separates organelles from cells. Ultracentrifugation uses very high speeds and forces particle separation. Centrifugation has many applications in areas like water treatment, biomedical research, and industries like sugar and oil production.Centrifugation
Centrifugationfarzeen javaid
油
The document discusses centrifuges and centrifugation. It begins by summarizing the early history of centrifuges, including inventions in the 18th and 19th centuries. It then provides definitions and explanations of key terms like centrifuge, centrifugation, and relative centrifugal force. The rest of the document details different types of centrifuges, components of centrifuges, separation techniques, and rotors and tubes used in centrifugation.Centrifugation techniques
Centrifugation techniquesAmany Elsayed
油
1. Instrumental analysis involves using instruments to convert physical or chemical characteristics of analytes into interpretable information. This includes clinical chemistry which aims to facilitate accurate diagnostic testing.
2. Centrifugation techniques use high centrifugal force to separate particles or molecules in a suspension more quickly than gravity alone. This involves spinning samples at high revolutions per minute in different types of centrifuges.
3. Centrifuges vary in size from microcentrifuges suitable for small volumes to preparative or analytical ultracentrifuges capable of very high speeds. The type used depends on the separation or analysis needed. All aim to control variables like speed, radius, and temperature to achieve reliable separations.Centrifugation notes slideshare
Centrifugation notes slideshare strawverrymilktae
油
Centrifugation is a technique that uses centrifugal force to separate components of a solution based on properties like density, size, and shape. It works by spinning the solution at high speeds, allowing heavier components to sediment to the bottom. There are two main types - density gradient centrifugation, where particles separate through a density gradient, and differential centrifugation, which separates organelles from cells. Centrifugation has many applications, including separating cream from milk, clarifying wine, and purifying proteins and cells.Centrifugation
CentrifugationOyshe Ahmed
油
Centrifugation is a process that uses centrifugal force to separate particles or molecules based on their size, shape, or density. It involves spinning a sample in a centrifuge to separate it into its components. There are various types of centrifugation classified based on speed, temperature, or separation method including differential, isopycnic, sucrose gradient, and ultracentrifugation. Centrifugation has many applications in industries like pharmaceuticals, water treatment, and oil extraction as well as in research areas like biochemistry and molecular biology.Chromatography and Its Types
Chromatography and Its TypesDr. Shabana Naz Shah
油
Chromatography is a technique used to separate and identify components of a mixture. It works by distributing molecules between a stationary and mobile phase. Molecules that spend more time in the mobile phase move faster through the column. There are several types of chromatography classified by mobile phase or separation mechanism, including gas chromatography which uses gases, thin layer chromatography which uses adsorbents on plates, and liquid chromatography which uses liquids. Chromatography is used in various applications such as pharmaceutical analysis, environmental monitoring, and forensic analysis.Centrifugation
CentrifugationDN Vaisnavi
油
This document discusses centrifugation and provides details about its history, basic principles, types of centrifuges and rotors, and applications. Centrifugation uses centrifugal force to separate mixtures based on density. It has been used since the 1400s and important developments include the first analytical ultracentrifuge in 1920. Common centrifuges include low-speed, high-speed, and ultracentrifuges, which separate particles based on mass and density. Rotors include swinging bucket, fixed angle, and vertical designs. Centrifugation has many clinical and laboratory applications, including separating blood components and subcellular particles.Electrophoresis and its application
Electrophoresis and its applicationDIPANKAR SAIKIA
油
Electrophoresis:
Electrophoresis is separation technique based on movement of charge particle in an electric field.
Movement of charge particles can be determined by following formula--
V= Eq/f
Where,
V= Velocity of the charged particle;
E= electric field of the molecule;
q= Net charge of the molecule; and
f= Frictional co-efficient of the molecule
Types of electrophoresis:
1. Agarose gel electrophoresis ;
2. Poly-acryl amide gel electrophoresis [PAGE];
3. Sodium do-decyl sulphate Poly- acrylamide gel electrophoresis [SDS-PAGE] ;
4. Two dimensional Poly-acrylamide gel electrophoresis [2D-PAGE];
5. Pulse field gel electrophoresis [PFGE];
6. Capillary gel electrophoresis [CGE]; and
7. Disc electrophoresis for Protein.
Application of electrophoresis:
1. Estimation of the DNA molecule.[ Agarose , PAGE ]
2. Analysis of PCR product. [ Agarose ]
3. Separation of restricted genomic DNA and RNA. [Agarose and PAGE respectively]
4. Conformation of newly isolated DNA .[Agarose]
5. Separation of most small fragments of DNA. [PAGE]
6. In forensic science.[Agarose , PAGE, SDS-PAGE, 2D PAGE ,Capillary gel electrophoresis , PFGE]
8. In determining molecular wt. of protein.[SDS-PAGE].etc
Types of centrifuges
Types of centrifugesShilpa Bhat
油
The document describes different types of centrifuges based on their design features and intended applications. Centrifuges vary in maximum speed, capacity, temperature control, and sample volume capabilities. Small benchtop centrifuges are used in clinical labs for blood separation and can hold around 100 tubes. Microcentrifuges are very common in biology labs, can hold small tube volumes, and generate forces up to 15,000g. High speed centrifuges spin at 15,000-20,000 RPM and are used for research applications requiring separation of cellular components. Ultracentrifuges provide the highest speeds and forces but are expensive and require special rotors and cooling due to heat generation.Polyacrylamide gel electrophoresis
Polyacrylamide gel electrophoresisUniversity of Mumbai
油
PAGE is a subtype of the gel electrophoresis whereby the normal gel is replaced with polyacrylamide gels use as the support matrix.
widely used and has very much importance.
COMPLETE PROCEDURE & USES are described in the slide.
Phase contrast & Flurosence microscope
Phase contrast & Flurosence microscopemicrobiology Notes
油
This document provides an introduction to phase contrast microscopy and fluorescent microscopy. It discusses that phase contrast microscopy, developed in 1934, uses optical techniques to produce high-contrast images of transparent samples like living cells. It works by converting differences in refractive index to intensity differences visible to the eye. Fluorescent microscopy illuminates samples with high-energy light, causing fluorophores to emit lower-energy light, which can then be filtered and observed. Both techniques allow viewing of unstained, living samples like cells in more detail.Ultracentrifugation
Ultracentrifugation Aswathi K S
油
The document discusses ultracentrifugation, which uses high centrifugal forces to separate particles in solutions based on size, shape, and density. It describes:
1) How particles experience centrifugal, buoyant, and frictional forces when spun in an ultracentrifuge.
2) Key terms like sedimentation rate, sedimentation coefficient, and angular velocity.
3) Types of ultracentrifugation experiments like sedimentation velocity and equilibrium experiments.
4) Types of preparative ultracentrifugation like differential, density gradient, zonal, and isopycnic centrifugation used to separate cell components.
5) Components of an ultracentrifuge like rotors, buckets,Centrifugation (Extraction Technique)
Centrifugation (Extraction Technique)Zoologist Pakistan
油
際際滷s about Centrifugation
際際滷s include Definition, History, Basic Principle, Instrumentation, Low Speed Centrifuges, High Speed Centrifuges, Ultra centrifuges, Types of Rotor, Preparative Techniques, Analytical Techniques, Applications of Centrifugation & Significance of centrifugationPAGE- Electrophoresis
PAGE- ElectrophoresisTapeshwar Yadav
油
It is a subtype of the gel electrophoresis whereby the normal gel is replaced with polyacrylamide gels used as support media.
Gels are made by free radical-induced polymerization of acrylamide and N,N-Methylenebisacrylamide.
It is the most widely used technique of electrophoresis.
Principles and application of chromatography
Principles and application of chromatographysuniu
油
Chromatography is a laboratory technique used to separate mixtures by distributing components to separate between two phases, one stationary and one mobile. It works based on how substances partition between the phases and move through the system at different rates. Common techniques include column chromatography, thin layer chromatography, gas chromatography, and high performance liquid chromatography. Chromatography is used in analytical chemistry to identify unknown substances and quantify components in a mixture.PHASE MICROSCOPY
PHASE MICROSCOPYRajpal Choudhary
油
This document discusses phase contrast microscopy. It begins by defining a microscope and microscopy. It then describes the main types of microscopes, including optical, electron, and scanning probe microscopes. It focuses on the light microscope, explaining that it uses visible light and is commonly used in biology to view structures like cells. It defines magnification and resolution. It describes the different modes of light microscopy - bright field, dark field, and phase contrast - and explains that phase contrast microscopy allows observation of living cells by enhancing contrast between structures with small refractive index differences. It notes that phase contrast microscopy was invented by Frits Zernike, for which he won the Nobel Prize in Physics in 1953.Centrifugation
Centrifugation Sajad Rather
油
Centrifugation is a procedure that uses centrifugal force to separate mixtures. Denser components move away from the axis of rotation while less dense components move towards the axis. The document discusses the principles, types (low speed, high speed, ultracentrifuges), applications, and techniques (preparative, differential, density gradient) of centrifugation. It provides details on rotor types, speeds, uses for separating organelles, macromolecules, and more. Diagrams illustrate basic centrifuge components and a table compares characteristics of different centrifuge types.Similar to Ppt of centrifugation (20)
Centrifugation techniques by kk sahu
Centrifugation techniques by kk sahuKAUSHAL SAHU
油
Introduction
Definition
Components of centrifuge
Types of centrifugation
Application
Conclusion
References
A centrifugation is a technique for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed.
Centrifugation
CentrifugationBibatsu
油
Centrifugation is a process that uses centrifugal force to separate mixtures of particles based on density. It works by spinning a sample rapidly, causing denser particles to migrate outward while less dense particles move inward. There are various types of centrifuges and rotors that can be used for different applications like separating blood components, purifying proteins, or clarifying wine. Common techniques include differential centrifugation to separate cell components, density gradient centrifugation to separate mixtures based on buoyant density, and ultracentrifugation for high-speed separations. Centrifugation is widely used in industries like water treatment and laboratories for analytical purposes.Centrifugation
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Centrifugation is a process that uses centrifugal force to separate mixtures based on density. It works by spinning a liquid sample at high speeds, causing denser components to migrate outward and separate from less dense components. There are several types of centrifugation including density gradient centrifugation, differential centrifugation, and ultracentrifugation. Centrifugation has many applications such as separating immiscible liquids, purifying cells, and separating blood components.Centrifugation Presentation.pptx
Centrifugation Presentation.pptxAnishaKazi
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A centrifuge operates by using the sedimentation principle- Here the substances are separated based on their density under the influence of gravitational force. When spun rapidly, lighter particles stay at the top and heavier particles go to the bottom during centrifugation.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.The components of heterogeneous mixtures are detached by centrifugation. That comprises liquids in liquids, solids in fluids, and gases in solids and liquids. In order to transfer bulky sections to the outside of the pipe, centrifugation uses centrifugal energy. It allows the solid to settle more easily and completely.
What is the centrifuge used for?
Centrifuges work by separating out two materials with different densities. Centrifuges are used in various laboratories to separate fluids, gases or liquids based on density like the separation of different constituents of blood, immiscible liquids, wastewater sludge etc.centrifugation ( terminology,introduction).pptx
centrifugation ( terminology,introduction).pptxGaurangiMahla
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centrifugation
( terminology,introduction)Centrifuge.pptx
Centrifuge.pptxsaraso888
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Centrifugation is a process that uses centrifugal force to separate mixtures based on density. Particles of different masses will settle at different rates in response to gravity when placed in a centrifuge. There are several types of centrifuges including low-speed, high-speed, and ultracentrifuges that can generate different centrifugal forces. Centrifugation techniques like density gradient centrifugation and differential centrifugation are used to separate components of a mixture based on properties like size, shape, and density. Centrifugation has many applications in clinical and research laboratories, such as separating blood components and isolating organelles or other cellular components.Centrifugation
CentrifugationPharmacy Universe
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Centrifugation is a technique used for the separation of particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. The particles are suspended in a liquid medium and placed in a centrifuge tube. The tube is then placed in a rotor and spun at a define speed.Centrifugation-Basic study
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This PPt is useful for the people who want to study the basics and the types of centrifugation and parts of the centrifugeExtraction techniques
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I AM HAFIZ MUHAMMAD WASEEM from mailsi vehari
BSc from science college Multan
MSC university of education Lahore
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i am HAFIZ M WASEEM from mailsi vehari
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msc univesity of education lahore pakistan
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This document summarizes a presentation on centrifugation given by Bhavika B. Katariya. It defines centrifugation as a process that uses centrifugal force to separate materials of different densities in a suspension. The key principles of centrifugation are explained, including how centrifuges use very strong centrifugal forces compared to gravity alone to separate particles based on density differences. The main types of centrifugation are described as density gradient, differential, and ultra centrifugation. Applications of centrifugation in various fields like biology, chemistry and forensics are also listed.Centrifugation
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Centrifugation is a technique used to separate particles in a solution based on their density. It works by applying centrifugal force using a centrifuge. There are different types of centrifuges that separate particles at varying speeds, including low-speed centrifuges up to ultracentrifuges that can spin at over 65,000 RPM. The principle involves particles of higher density sinking to the bottom while lower density particles float to the top. Centrifugation is used in clinical and research applications to separate blood components, subcellular organelles, proteins, and more.Centrifugation
CentrifugationShahedShadin
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It's a topic of pharmaceutical engineering. In pharma industry,we need to separate each and every particles.Centrifugation is the process and this process will happen through centrifuge machine.That time,centrifugation process is hardly needed.There has different type of centrifuge machine.Centrifugation basic principle & theory
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Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. It is a method to separate molecules based on their sedimentation rate under the centrifugal field. It involves the use of centrifugal force for the sedimentation of molecules.centrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-16012509360...
centrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-16012509360...sv197679
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centrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copy-160125093608 A laboratory screening method used in plant breeding is marker-assisted selection, which uses molecular markers or DNA fingerprinting to identify genes that are responsible for a desired trait. This method allows plant breeders to screen large populations of plants for the desired trait, rather than visually identifying it.Field screening is a plant breeding technique that involves evaluating varieties for resistance to pests and diseases in natural fields. Here are some ways to conduct field screening:Greenhouse screening is a plant breeding technique that involves testing plants for pest and disease resistance in a controlled environment:Sow or plant earlyInterplant susceptible plantsUse insect attractantsUse cagesDate:
Page No
feld Screening
field Screening is a plan technique Plant breeding evaluting varieties evaluting
resistance to pests and Conduct field Screening. diseases to
Create an infested environment
use insect attractants.
use Cages
Interplant susceptible plants
Low ar plant earlyCreate an infested environmentA laboratory screening method used in plant breeding is marker-assisted selection, which uses molecular markers or DNA fingerprinting to identify genes that are responsible for a desired trait. This method allows plant breeders to screen large populations of plants for the desired trait, rather than visually identifying it.Field screening is a plant breeding technique that involves evaluating varieties for resistance to pests and diseases in natural fields. Here are some ways to conduct field screening:(1).pdfcentrifugationanu-copy-160125093608centrifugationanu-copy-160125093608 (1).pdfGreenhouse screening is a plant breeding technique that involves testing plants for pest and disease resistance in a controlled environment:A laboratory screening method used in plant breeding is marker-assisted selection, which uses molecular markers or DNA fingerprinting to identify genes that are responsible for a desired trait. This method allows plant breeders to screen large populations of plants for the desired trait, rather than visually identifying it.Field screening is a plant breeding technique that involves evaluating varieties for resistance to pests and diseases in natural fields. Here are some ways to conduct field screening:centrifugationanu-copy-160125093608 (1).pdf(1).pdfcentrifugationanu-copy-160125093608(1).pdfcentrifugationanu-copy-160125093608 (1).pdfcentrifugationanu-copBioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...The University of Lahore
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This document provides an overview of centrifugation as a method to separate particles in a solution. It discusses the basic principles, including that centrifugation uses centrifugal force to accelerate the sedimentation of particles based on properties like density, size, and shape. It separates components faster than simple gravitational sedimentation. The document also outlines the interacting forces involved, such as buoyant force and frictional force, and provides examples of calculating centrifugal force and field.Centrifugation
CentrifugationNishant kumar
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Centrifugation is a separation technique that uses centrifugal force to separate mixtures based on density differences. It works by spinning samples at high speeds, causing denser components to sediment faster and separate out from less dense components. The basic components of a centrifuge are an electric motor to spin the samples and a rotor to hold the sample containers. There are different types of rotors and centrifugation techniques that separate samples based on factors like density, size, or sedimentation rate. Proper use and maintenance of centrifuges and rotors is important for safety and instrument lifespan.Centrifugation
Centrifugationkhadeeja ikram01
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Centrifugation is a process that uses centrifugal force to separate mixtures of substances based on density differences. It involves spinning a sample in a centrifuge which causes denser components to migrate outward while less dense components migrate inward. There are various types of centrifugation techniques used for separation in industrial and laboratory settings, including differential centrifugation, density gradient centrifugation, and ultracentrifugation. Centrifugation has many applications such as separating solids from liquids in water treatment, separating blood components, and separating particles in industrial processes.Centrifugation amirtham
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A centrifuge uses centrifugal force to separate fluids or particles of different densities. It works by applying centrifugal acceleration, which causes denser particles to move outward in the radial direction while less dense particles move to the center. There are different types of rotors and centrifuges that separate particles based on properties like size, shape, density or sedimentation rate. Ultracentrifuges spin at very high speeds of over 100,000g to separate small particles like organelles, viruses and macromolecules.prvnppt-141230053156-conversion-gate02.pdf
prvnppt-141230053156-conversion-gate02.pdfDevPanda5
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Centrifugation is a process that uses centrifugal force to separate particles in a solution based on their density. It can be used to sediment particles, isolate cellular components like organelles, and separate molecules and complexes. Different types of centrifuges include low-speed, high-speed, and ultracentrifuges, which separate particles through differential centrifugation or density gradient centrifugation. Analytical centrifugation allows observation of fractionation processes and is used to study macromolecules. Centrifugation has various applications including concentration, separation, isolation of organelles, and separation of blood components or cream from milk.BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...The University of Lahore
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- 1. CENTRIFUGATION Submitted by SHOBHANA SAHU M.Sc. 4th semester 1
- 2. SYNOPSIS Introduction Definition Components of centrifuge Types of centrifugation Application Conclusion References 2
- 3. INTRODUCTION Centrifugation is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, used in industry and laboratory settings. This process is used to separate two immiscible liquids. 3
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- 5. DEFINITION A centrifugation is a technique for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. 5
- 6. CENTRIFUGE A centrifuge is a part of equipment that puts an object in rotation around a fixed axis (spin in a circle), applying a potentially strong force perpendicular to the axis of spin. 6
- 7. PRINCIPLE In a solution, particles whose density is higher than that of the solvent sink (sediment), and particles that are lighter than it float to the top. The greater the difference in density, the faster they move. If there is no difference in density (isopycnic conditions), the particles stay steady. To take advantage of even tiny differences in density to separate various particles in a solution, gravity can be replaced with the much more powerful centrifugal force provided by a centrifuge. 7
- 8. COMPONENTS OF CENTRIFUGE Motor- which give rotator motion. Drive shaft- attached to motor. Rotor- placed on drive shaft having space to place tubes. Centrifuge rotor: a rotor is the rotating unit of the centrifuge, which has fixed holes drilled at an angle. Test tubes are placed inside these holes and the rotor spins to aid in the separation of the materials. 8
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- 12. TYPES OF CENTRIFUGATION Preparative centrifugation: it is a preliminary step to separate component for further separation and analysis. It can handle large amount of samples. Its divide 2 types: Density gradient centrifugation Differential velocity centrifugation Analytical centrifugation: we analyze the sample so small amount is used few micro-gram/ L to 1 mL Mostly ultracentrifuge is used 12
- 13. DENSITY GRADIENT CENTRIFUGATION Under centrifugal force, the particles will begin sedimenting through the density gradient. Two type of this- 1. Rate zonal centrifugation 2. Isopycnic centrifugation 13
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- 20. ULTRACENTRIFUGATION The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 19600 km/s族 (around 50000 rpm). Example: Red cells may be separated from plasma of blood, nuclei from mitochondria in cell homogenates, and one protein from another in complex mixtures. 20
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- 25. APPLICATION OF CENTRIFUGATION Isolating suspensions Isotope separation Aeronautics and astronautics Geotechnical centrifuge modeling: Commercial applications: 25
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- 29. CONCLUSION The centrifugation is a modern and easy technique of separation. Due to centrifugation it is easy to separate cellular and sub cellular components. It is used to study the effects of centrifugal forces on cells. But it is costly and problem to maintenance. 29
- 30. REFERENCES /poojakamble1609/principles-and-applications-of-centrifugation /poojakamble1609/principles-and-applications-of-centrifugation https://www.britannica.com/technology/centrifuge https://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-separations.html http://www.centrifuge.guide/significant-applications-of-centrifuges/ http://centrifuges.hitachi-koki.com/application/pdf/red153e.pdf http://www.phys.sinica.edu.tw/TIGP- NANO/Course/2007_Spring/Class%20Notes/AC_Chapter%203%20Centrifugation%200321.pdf 30