Prasetation
Biotransformation is a biological process where an organic compound is modified into a reversible product through simple, chemically defined reactions catalyzed by enzymes in microbial, plant, or animal cells. Microbial cells have advantages for biotransformation due to their high surface area to volume ratio, rapid growth, and high metabolic rates that allow for efficient substrate transformation. Biotransformation can use growing, resting, or dried cells, or isolated enzymes, with substrates transformed in agitated fermentation vessels or packed columns for continuous operation. Immobilizing cells on solid supports can increase stability and prolong operation. Biotransformation has also been used successfully with plant cells for various novel compounds.
Prasetation
- 2. biotransformation Biotransformation is biological process whereby an organic compound is modified into reversible product. These involve simple, chemically defined reaction catalysed by enzyme present in the cell. ( eg, microbial, plant and animal)
- 3. Microbial cell have advantage over plant and animal cell due to their high surface volume ratio, rapid growth high rates of metabolism for the efficient transformation of the substetem added. The substrate to be transfarmed can be growing cell, resting cell, dried cell, or to ezyme isolate from these cell. Forming meadium shoud be agitated. Aerated fermentir or packed in column for continous opration.
- 4. The can be immoblisedon solid support increase there stability and prolonger operation. Biotransformation were carried with plant cell successfully for a varieties of novel compounds, eg cardiac glycoside, digoxin, benzyl isoquinoline etc. Biotransformatn in plant cell congugated reaction, these cellore superior to microoranism