Prasetation
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Biotransformation is a biological process where an organic compound is modified into a reversible product through simple, chemically defined reactions catalyzed by enzymes in microbial, plant, or animal cells. Microbial cells have advantages for biotransformation due to their high surface area to volume ratio, rapid growth, and high metabolic rates that allow for efficient substrate transformation. Biotransformation can use growing, resting, or dried cells, or isolated enzymes, with substrates transformed in agitated fermentation vessels or packed columns for continuous operation. Immobilizing cells on solid supports can increase stability and prolong operation. Biotransformation has also been used successfully with plant cells for various novel compounds.
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Bio- catalysis.pptxNitin Pandey
油
The document discusses bio-catalysis and the use of enzymes in organic synthesis. It notes that bio-catalysts are derived from renewable resources, are biodegradable, and allow reactions to proceed under mild conditions. Examples are given of green bio-catalytic processes developed by Pfizer and Codexis for manufacturing pharmaceuticals. The types of bio-catalyst enzymes are described along with their advantages over traditional chemical catalysts. Methods of immobilizing enzymes on supports are summarized, including entrapment, cross-linking, and attachment to porous or nano-structured materials.MEDICINAL PLANT BIOTECHNOLOGY-IMMOBILIZATION,MICROPROPAGATION-M.PHARM-II-SEME...
MEDICINAL PLANT BIOTECHNOLOGY-IMMOBILIZATION,MICROPROPAGATION-M.PHARM-II-SEME...jeeven 427
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raditionally, four methods are used for enzyme immobilization, namely (1) adsorption (2) physical entrapment, (3) covalent attachment (4) encapsulation
(5)crosslinking. Micropropagation is the artificial process of producing plants vegetatively through tissue culture or cell culture techniques.Meristem Culture
Callus Culture
Suspension Culture
Embryo Culture
Protoplast Culture these are discussed.
An overview of the animal & plant cell reactors used in laboratories
An overview of the animal & plant cell reactors used in laboratoriesErin Davis
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This is a power point presentation which illustrates a brief idea about the cell culturing in the animal and plant cell reactors.Cell Immobilization Methods and Applications.pptx
Cell Immobilization Methods and Applications.pptxCherry
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Cell Immobilization Methods and Applications.pptxPlant Cell Immobilization.pptx
Plant Cell Immobilization.pptxChhavi Singh
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Plant cells can be immobilized through various methods like surface attachment, entrapment in porous matrices, containment behind barriers, and self-aggregation. This allows maintaining high cell densities to increase productivity of secondary metabolites. Immobilization provides advantages like easier product separation, continuous processing, and protecting cells from shear forces. However, limitations include additional costs, complexity in understanding plant cell pathways, and potential loss of biosynthetic capacity. Applications of immobilized plant cells include production of high-value compounds, biotransformations, and synthetic seed technology.Embryo rescue, Somaclonal Variation, Cryopreservation
Embryo rescue, Somaclonal Variation, CryopreservationAbhinava J V
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This document discusses various techniques in plant biotechnology including embryo rescue, somaclonal variation, and cryopreservation. Embryo rescue involves culturing immature or weak embryos on artificial nutrient media to allow their development. Somaclonal variation refers to genetic and phenotypic changes that can occur in plants regenerated from tissue culture. Cryopreservation aims to preserve plant cells and tissues in a frozen state at ultra-low temperatures like liquid nitrogen. The key steps involve adding cryoprotectants, freezing, storage, thawing, and regeneration of plants. These techniques have various applications for breeding programs and conservation of plant genetic resources.microbialtransformation-Class.ppt Biotechnologyx
microbialtransformation-Class.ppt Biotechnologyxrakeshbarik8
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This document discusses microbial biotransformation, which uses microorganisms like bacteria and fungi to enzymatically modify organic compounds. Biotransformation is advantageous because the enzymes act specifically under mild conditions. The process typically involves growing microbes in a growth phase, then adding the target compound in a transformation phase. Common reactions include oxidation, reduction, and hydrolysis. Biotransformation is used to produce steroids, prostaglandins, modified antibiotics, and break down pollutants like petroleum for environmental remediation.Plant tissue culture
Plant tissue culturePraveen Garg
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This document provides information about plant tissue culture techniques. It discusses that plant tissue culture involves culturing small explant tissues in a sterile nutrient medium under controlled conditions. With the addition of hormones, new shoots and roots can be induced to grow from explants. Tissue culture is used for micropropagation to produce clones of plants. Single plant cells also have the ability to regenerate into whole plants given the right conditions. The document outlines the composition of solid and liquid culture media, different tissue culture techniques, types of culture, advantages, applications and nutritional requirements for plant tissue culture.Immobilization of cells 
Immobilization of cells Dr Suresh Solleti
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Immobilization of cells involves encapsulating cells in polymers to prevent division while maintaining viability. This allows increasing cell density in bioreactors for higher metabolite production. Methods include entrapment in gels, on fiber mats, or behind semi-permeable membranes. Alginate is commonly used for gel entrapment. Immobilized systems offer advantages like prolonged biomass use, higher product yields, and genetic stability. They also simplify downstream processing when products are extracellular. Various bioreactor designs are used depending on the immobilization method. Applications show enhanced production of metabolites like serpentine, anthraquinones, and capsaicin using immobilized plant cells.Suspension Culture and Single Cell Cultures, Culturing methods, maintenance a...
Suspension Culture and Single Cell Cultures, Culturing methods, maintenance a...Ananya Sinha
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Suspension Culture and Single Cell Cultures, Culturing methods, maintenance and application
Generally, suspension culture is a one stop technology to produce secondary metabolites on a large scale in-vitro, irrespective of the climatic condition or nutrient availability (as required in field plants).
In this presentation, we will see the importance of suspension culture, culturing methods and it's application (mostly with respect to plants) and also focus on what exactly is a single cell culture.Prasetation
- 2. biotransformation Biotransformation is biological process whereby an organic compound is modified into reversible product. These involve simple, chemically defined reaction catalysed by enzyme present in the cell. ( eg, microbial, plant and animal)
- 3. Microbial cell have advantage over plant and animal cell due to their high surface volume ratio, rapid growth high rates of metabolism for the efficient transformation of the substetem added. The substrate to be transfarmed can be growing cell, resting cell, dried cell, or to ezyme isolate from these cell. Forming meadium shoud be agitated. Aerated fermentir or packed in column for continous opration.
- 4. The can be immoblisedon solid support increase there stability and prolonger operation. Biotransformation were carried with plant cell successfully for a varieties of novel compounds, eg cardiac glycoside, digoxin, benzyl isoquinoline etc. Biotransformatn in plant cell congugated reaction, these cellore superior to microoranism