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Presentation Sirion Web V7 Spoken

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biofrost

Sirion offers genetically modified cell services including cell line optimization, immortalization, and gene overexpression or silencing using viral vectors like adenovirus and lentivirus. They guarantee delivery of any gene to any mammalian cell type and greater than 90% knockdown maintained over experimental timeframes. Sirion claims to provide customized genetically modified cells, viruses, and services in weeks rather than the months needed for in-house solutions.

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Experts in Gene Silencing and Over-expressionDelivering ANY Gene to ANY Cell
Sirion OffersShorter Timelines andImproved QualityCustom development of genetically modified mammalian cellsCell line optimization servicesCell line immortalization services
Sirion DeliversShorter Timelines andImproved QualityFunctionally tested, ready-to-use tailor-made genetically modified cells, Guaranteed gene delivery into virtually any mammalian cell typeGuaranteed > 90% knockdown maintained over the required experimental time frameResults in weeks instead of the months needed for in-house solutions
Our expertiseViral vectors ─ Adenovirus, AAV, Lentivirus For cell research and vaccine developmentGenetically modified cell systems for target validation Cellular models for oncology
Ready-to use frozen cells
Immortalization of primary human cells Optimization of production cells Rational target identification by dynamic mRNA profilingOur expertise: Virus technologyAAVAdenovirusLentivirusBasic ResearchVaccine DevelopmentSafe, replication defective vectorsCustom construction and production, or direct supply from stockHigh titer stocks for in vivo use available
Guaranteed custom virus vectorsCustom virus vector constructionshRNA expression with certified knockdown efficiency
Flexible choice of promoters and expression cassettes
Expression up to 4 genes in one vector possible
QC, RCA-tested vector preparationsHigh titer virus stocks for in vivo Your BenefitsAll vectors are guaranteed for functionS1/BSL1 ready-to-use, pre-transduced, frozen cell stocks availableFast time lines ─ 4 weeks from gene to vectorSIRION-Viral vectorsSIRION BIOTECHState of the art transfectionMolecular Myology, LMU Munich
20 to 50 fold higher gene transferTransMAX Transduction ReagentGFP Transduction of NIH-3T3 without with TransMAX20 to 50-fold enhanced adenovirus-mediated gene expression
Increased gene transfer in vivo
Accessibility of cell types MOI 200 MOI 200NIH-3T3 cells were transduced with an Adenovirus Serotype 5 expressing EGFP with a MOI of 200 without and in the presence of TransMax.
Our expertise: Cell technologyHigh performance shRNAs with >90% knockdown efficiency

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Presentation Sirion Web V7 Spoken

  • 1. Experts in Gene Silencing and Over-expressionDelivering ANY Gene to ANY Cell
  • 2. Sirion OffersShorter Timelines andImproved QualityCustom development of genetically modified mammalian cellsCell line optimization servicesCell line immortalization services
  • 3. Sirion DeliversShorter Timelines andImproved QualityFunctionally tested, ready-to-use tailor-made genetically modified cells, Guaranteed gene delivery into virtually any mammalian cell typeGuaranteed > 90% knockdown maintained over the required experimental time frameResults in weeks instead of the months needed for in-house solutions
  • 4. Our expertiseViral vectors ─ Adenovirus, AAV, Lentivirus For cell research and vaccine developmentGenetically modified cell systems for target validation Cellular models for oncology
  • 5. Ready-to use frozen cells
  • 6. Immortalization of primary human cells Optimization of production cells Rational target identification by dynamic mRNA profilingOur expertise: Virus technologyAAVAdenovirusLentivirusBasic ResearchVaccine DevelopmentSafe, replication defective vectorsCustom construction and production, or direct supply from stockHigh titer stocks for in vivo use available
  • 7. Guaranteed custom virus vectorsCustom virus vector constructionshRNA expression with certified knockdown efficiency
  • 8. Flexible choice of promoters and expression cassettes
  • 9. Expression up to 4 genes in one vector possible
  • 10. QC, RCA-tested vector preparationsHigh titer virus stocks for in vivo Your BenefitsAll vectors are guaranteed for functionS1/BSL1 ready-to-use, pre-transduced, frozen cell stocks availableFast time lines ─ 4 weeks from gene to vectorSIRION-Viral vectorsSIRION BIOTECHState of the art transfectionMolecular Myology, LMU Munich
  • 11. 20 to 50 fold higher gene transferTransMAX Transduction ReagentGFP Transduction of NIH-3T3 without with TransMAX20 to 50-fold enhanced adenovirus-mediated gene expression
  • 13. Accessibility of cell types MOI 200 MOI 200NIH-3T3 cells were transduced with an Adenovirus Serotype 5 expressing EGFP with a MOI of 200 without and in the presence of TransMax.
  • 14. Our expertise: Cell technologyHigh performance shRNAs with >90% knockdown efficiency
  • 15. Inducible knockdown via partnership with TET systemsshRNA design and cloning into Sirion screening systemDetermination ofknockdown efficiency by QPCRConfirmation of knockdown on the protein levelby Western blotAd368-highperformance shRNA>90% KD
  • 16. >90% knockdown – Guaranteed! Shorter Timelines andImproved QualityHigh performance viral vectors with guaranteed >90% gene knockdownStable or inducible knockdown cells for reliable resultsShort timelines save money compared to long in house development Sirion’s experienced team can quickly generate the cell model you need
  • 17. More predictive screeningPrimary cell line generationPermanent Human Primary Cell immortalization
  • 18. Polyclonal or monoclonal cell line generation
  • 19. Phenotypic and functional characterizationConditional primary cell immortalizationThe immortalizing gene is active at 33°C / inactive at 37°C
  • 20. Conditionally immortalized cells terminally differentiate with close to primary cell type phenotype and function for more reliable results.* hTERT requires a license from Geron Inc.
  • 21. Validated frozen cells direct to your labValidated gene knock-down or over expression
  • 23. Standardized product (cell passage number, cell physiology, gene expression / knock-down) ApplicationsInfectious disease research
  • 25. Target validation in vivo (tumor models)
  • 26. Production cell line optimizationOur expertise: Cell line optimizationOptimization of production cell lines for vaccines and antibodies Target GeneIdentificationMission® CellsFunctionalTestingProductionCell Line Target ValidationTarget IdentificationCellular AssaysOptimizedProduction cellRational choiceof target genes by dynamic mRNA profiling
  • 27. Identification ofcritical cellular pathways for productivity
  • 28. Over-expression or knockdown of target genes
  • 35. Gene SilencingSirion DeliversShorter Timelines andImproved QualityFunctionally tested, ready-to-use tailor-made genetically modified cells, Guaranteed gene delivery into virtually any mammalian cell typeGuaranteed >90% knockdown maintained over the required experimental time frameResults in weeks instead of the months needed for in-house solutions
  • 36. Experts in Gene Silencing and Over-expressionDelivering ANY Gene to ANY CellSIRION BIOTECH GmbH Tel: +49 89 700 961 999Am Klopferspitz 19 Fax: +49 89 700 961 99882152 Martinsried info@sirion-biotech.comGermany www.sirion-biotech.com

Editor's Notes

  1. Virus vectors for basic research and vaccine development include Adenovirus vectors, Adeno-associated virus vectors, and lentivirus vectors for experimental gene transfer and target validation in cell culture and in vivo. Viral vectors produced by Sirion are safe and replication defective and can be used in vitro or supplied as high titer stocks for use in vivo. Sirion specializes in customized viral vector construction and production, but also supplies vectors direct from stock.. Each vector type has its individual benefits and application spectrum: