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Study of Antioxidants of Strawberry and
their effects on human health

Introduction
? Strawberry is an accessory fruit
? Strawberries belong to the Rosaceae family
? Perennial herb stimulated by winter weather

Phytochemicals
? Phenolic acids
? Flavonols
? Vitamin c
? Ellagic acid
? Carotenoids
? Anthocyanins
? Folate

Health Benefits
? Anti-Cancer
? Anti-Diabetic
? Anti-Ageing
? Fight bad cholesterol
? Anti-Inflammatory
? Boost Immune system
? Anti-Bacterial
? Anti-Viral
? Regulate Blood Pressure
? Lower the risk of cardiovascular diseases

Aim of project
? Figure out antioxidants activity and phenolic contents of
strawberry.
? Explore anti-diabetic & anticancer activities of mature
strawberry fruit.
? Investigate the folate effects of strawberry on
cardiovascular diseases.

Antioxidant action of Vitamin C,
carotenoids & Phenols.

Anticancerous activity of
polyphenols.

Folate’s effects on cardiovascular
diseases.
.
? Folate helps maintain normal levels of homocysteine,
an amino acid that may be an independent risk factor
for cardiovascular disease. Low dietary folate intakes,
low circulating folate levels or high plasma total
homocysteine concentrations have been associated
with an increased risk of coronary artery disease.

Material and Methods
? The experiment will be carried out in laboratory. First of all
sample will be prepared and extract will be prepared of
fresh strawberry fruit. Fractionation will be done by Solid
Phase extraction (SPE).
? Folin ciocalteu reagent will be used for the measurement of
total phenolic contents of strawberry fruit.
? Liquid chromatography and HPLC will be done for
extraction of Vitamin C & Folate present in strawberry
fruit.
? Anthocyanins contents will be determined by PH
Differential method.

Continue..
? Stable radical assay DPPH (1,1-diphenile-2-picrylhydrazile
radical) and FRAP (Ferric reducing antioxidant power)
techniques will be performed for evaluation of free radical
scavenging activity.
? To check the anti-diabetic activities of strawberry fruit,
Alpha-amylase inhibition assay and alpha-Glucosidase
inhibition assay will be used.

Statistical Analysis
? The results are expressed as mean ± standard
deviation of three replicates. The data was analyzed
for comparison by applying Tuky’s multiple range test
at 95% confidence level (p ≤ 0.05) using statistical
software SPSS version 19.0.

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