Pt2 nanopore
- 1. 弌亠仄舒 仗仂仂从仂仍舒 Rapid Sequencing 丐舒亞仄亠仆舒亳 亳亳于舒仆亳亠 舒亟舒仗亠仂于 亠从于亠仆亳仂于舒仆亳
- 2. 丼仂 仆亢仆仂 亟仍 亰舒仗从舒 Rapid?
- 3. 丼仂 仆亢仆仂 亟仍 亰舒仗从舒 Rapid?
- 4. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation
- 5. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation Covaris g-Tube 亳仍亳 仗亳
- 6. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation Covaris g-Tube 亳仍亳 仗亳 21G 20 舒亰 亳仍亳 26G 3-5 舒亰
- 7. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation (FFPE Repair Mix) End repair / dA-tailing module Blunt/TA Ligase Mix AMPure beads
- 8. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation (FFPE Repair Mix) End repair / dA-tailing module Blunt/TA Ligase Mix AMPure beads
- 9. 弌亠仄舒 仗仂仂从仂仍舒 1D Genomic DNA by ligation
- 10. 亳从舒 弍亳弍仍亳仂亠从亳 SPRI bead technology: Solid Phase Reversible Immobilization
- 11. 亳从舒 弍亳弍仍亳仂亠从亳
- 12. 仂仂从仂仍 1D^2 by ligation
- 13. 弌亠仄舒 仗仂仂从仂仍舒 1D^2 by ligation
- 14. 弌亠仄舒 仗仂仂从仂仍舒 1D^2 by ligation
- 15. 舒从仂亟亳仂于舒仆亳亠 弍亳弍仍亳仂亠从 亳亞亳仂于舒仆亳亠 弍舒从仂亟仂于 Native barcoding kit 亟仍 1D
- 16. 舒从仂亟亳仂于舒仆亳亠 弍亳弍仍亳仂亠从 亳亞亳仂于舒仆亳亠 弍舒从仂亟仂于 Native barcoding kit 亟仍 1D 亳仂亠亟亳仆亠仆亳亠 舒仆仗仂亰仂仄仆仄 从仂仄仗仍亠从仂仄 Rapid Barcoding Sequencing Kit
- 17. 舒从仂亟亳仂于舒仆亳亠 弍亳弍仍亳仂亠从 亳亞亳仂于舒仆亳亠 弍舒从仂亟仂于 Native barcoding kit 亟仍 1D 亳仂亠亟亳仆亠仆亳亠 舒仆仗仂亰仂仄仆仄 从仂仄仗仍亠从仂仄 Rapid Barcoding Sequencing Kit 舒从仂亟 仆舒 丶-仗舒亶仄亠舒 PCR Barcoding Kit
- 18. 仂仄于舒仆亳亠 亠亶从亳 Flow Cell Wash Kit 舒于仂 仂亟亠仍磳 弍亳弍仍亳仂亠从 仂 仄亠仄弍舒仆 舒于仂 仗仂仄于舒亠 亠亶从 亟仍 仆亠仄亠亟仍亠仆仆仂亞仂 亳仗仂仍亰仂于舒仆亳 舒于仂 S 仗仂仄于舒亠 亠亶从 亳 仗仂亰于仂仍磳 舒仆亳 亠亠
- 19. 弌亠仄舒 仗仂仂从仂仍舒 1D Amplicon by ligation
- 20. 弌亠仄舒 仗仂仂从仂仍舒 1D Amplicon by ligation
- 21. 仂仄于舒仆亳亠 亠亶从亳 Flow Cell Wash Kit
- 22. 丐亠弍仂于舒仆亳 从 从舒亠于 磌亳仂舒 仗仂 Nanodrop: OD 260/280 于 仗亠亟亠仍舒 1,8 2,0 OD 260/230 仆亠 仆亳亢亠 2.0 亠亰 亟亠亠亞亠仆仂于 亳 亟亞亳 舒亰仄亠 舒亞仄亠仆仂于 >30kb 碻仂仂仆仂亠仆亳亠 从仂仆亠仆舒亳亶 仂亞仍舒仆仂 Qubit 亳 Nanodrop 亟仂仍亢仆仂 弍 仆亠 弍仂仍亠, 亠仄 1 : 1,5
- 23. 丐亠弍仂于舒仆亳 从 从舒亠于 丼亳仂舒 仗仂 Nanodrop: OD 260/280 于 仗亠亟亠仍舒 1,8 2,0 OD 260/230 仆亠 仆亳亢亠 2.0
- 24. 亟仂仍亢仆舒 弍 于仂从仂仄仂仍亠从仍仆仂亶 https://twitter.com/baptistemayjon1/status/818484473780203520 仍亳仆舒 仗仂亠仆亳 == 亟仍亳仆舒 舒亞仄亠仆舒
- 25. 弌从仂仍从仂 仆舒亟仂 亟仍 仗仂仂从仂仍仂于 弍亠亰 丶? Rapid 1D 1D^2 Direct cDNA Direct RNA 400 仆亞 1 - 2 仄从亞 1 - 2 仄从亞 250 仆亞 500 仆亞 仂亳亠仆仆仂亶 仂
- 26. 弌从仂仍从仂 仆舒亟仂 亟仍 仗仂仂从仂仍仂于 弍亠亰 丶?
- 27. 丼舒 3.亳仄亠仆亠仆亳 仆舒仆仂仗仂仆仂亞仂 亠从于亠仆亳仂于舒仆亳. 仆仂于仆亠 舒亟亳亳 亠从于亠仆亳仂于舒仆亳 仆舒 Oxford Nanopore 仂仍仆仂亞亠仆仂仄仆仂亠 亠从于亠仆亳仂于舒仆亳亠: 仂亠亟亳仆磺 亳 于仍舒于亶 弌亠从于亠仆亳仂于舒仆亳亠 舒仄仗仍亳从仂仆仂于: 16S, 18S, 亳 亟亞亳亠 弌亠从于亠仆亳仂于舒仆亳亠 从 亳 仗礆仂亠 亠从于亠仆亳仂于舒仆亳亠 亠仂亳亳舒仍仆亠 仗仂亟从舒亰从亳: 从舒从 仗仂于亳 于舒亳 舒仆 仆舒 仗亠?
- 28. 3.1. 仆仂于仆亠 舒亟亳亳 亠从于亠仆亳仂于舒仆亳 仆舒 Oxford Nanopore
- 29. 亟亠仍亠仆亳亠 弌 仗仂仄仂 从仂仍仂仆仂从 30 kb
- 30. 亟亠仍亠仆亳亠 弌 仗仂仄仂 从仂仍仂仆仂从 Genomic tips 50-100 kb30 kb
- 32. 亟亠仍亠仆亳亠 弌 仗仂仄仂 亟亳舒仍亳亰舒 亟亠仍亠仆亳亠 于 舒亞舒仂亰亠 ~ 1000 kb150-250 kb
- 34. MinKNOW: 亠仆 仗舒于仍亠仆亳 亠从于亠仆舒仂仂仄
- 35. 仂于亠从舒 仗亳弍仂舒
- 36. 弌亠从于亠仆亳仂于舒仆亳亠