More Related Content
What's hot (16)
Similar to RPDResume 20Apr2016 (20)
RPDResume 20Apr2016
- 1. Raymond P Damien rpdamien@gmail.com 30 Glen Ave Fitchburg, MA 01420 mobile: (978) 790-7742 Continued SUMMARYOF EXPERIENCE  HPLC/UPLC (Reverse Phase,SPE,GPC)  Flash Column Chromatography (Purification)  TLC (Thin Layer Chromatography)  UV-Vis, Florescence (Florescamine), BCA,Micro BCA  Gel and Capillary Electrophoresis  DLS (Dynamic Light Scattering)  Equilibrium Dialysis  Cell line culture and maintenance  Method Development, and Validation  DoE (Design of Experiments)  In vitro Release Studies and Data Interpretation  Biologic and Drug Substance Encapsulation and Formulation  Rheology  Gel and Capillary Electrophoresis (SDS-PAGE and Agarose)  DNA Extraction and PCR  cGMP/GLP/GXP Environments  Automation and System Integration  SOP and Development Report writing  Trained in MA RCRA and DOT hazardous materials and chemical hygiene requirements EXPERIENCE Selecta Biosciences Research Associate Analytical Development Watertown, MA, July 2011 – February 2016  Developed and improved sample preparation and analysis methods of complex Synthetic Vaccine Particle components (Peptides and Proteins) by HPLC  Developed and improved sample preparation and analysis methods of single strand synthetic DNA (CpG) encapsulated nanoparticles by HPLC  Experienced with using gradient reversed-phase separations,creation of HPLC methods, preparations of mobile phases, and (Agilent 1100, 1260, and 1290 with Chemstation) HPLC maintenance  Used DLS (Dynamic Light Scattering) to determine particle size and polydispersity  Determined molecular weight by GPC (Gel Permeation Chromatography)  Protein analysis by SDS-PAGE and antibody binding affinity through Equilibrium Dialysis  Wrote SOP's and Reports to document test methods, procedures, OOT/OOS results, and technical documents  Trained in MA DOT regulations of 49 CFR 100-185, MA RCRA,and member of the Safety Committee  Served as analytical representative for 2 project teams Ocular Therapeutix (I-Therapeutix) Research Associate Research and Development/ Drug Delivery Bedford, MA, April 2008 – September 2010  Developed methods of encapsulating drugs to be delivered Zero Order using biocompatible polymers  Built MS Access database used to control chemical inventory, and synchronized it with electronic versions of all MSDS sheets  Provided initial preclinical work that changed company focus from stand-alone medical devices to incorporate drug delivery  Designed and developed hydrogel-based products using novel polymers and cross-linking technologies  Provided technical documents detailing the design and analysis to support design control
- 2. Raymond P Damien rpdamien@gmail.com Continued  Developed correlational In Vivo/In Vitro studies to document efficacy of drug candidates and determined the necessary response outputs  Coordinated removal of lab waste per OSHA/DOT requirements and, trained and certified for 49 CFR 172 Subpart H Xcellerex Research Associate Process Development Marlborough, MA, September 2007 – April 2008  Performed High Throughput Design of Experiments to design both Basal Growth Media and Feed Media for a CHO cell line  Cultured and maintained cell lines  Automated methods for conducting a Design of Experiments (DOE) using robots from Tecan and Caliper  Developed robotic Hit Picking methods for the selection of clones  Assisted in the development of a mini bioreactor  Integrated multiple robots to reduce the need for constant human interface in GMP environments Caliper Life Sciences Senior Product Test Scientist Research and Development Hopkinton, MA, December 2006 – July 2007  Developed qualification documents, IQ, OQ,and PQ for a robotic liquid handling system (Sciclone ALH 3000, Affymetrix Genechip Array Station, and Zephyr) and a capillary electrophoresis system (Lab Chip 90)  Worked closely with R&D and Manufacturing to develop and troubleshoot new hardware and software  Provided expertise to assist in applications development, and technical support  Developed test plans to document crucial changes necessary to successfully launch a product  Performed statistical analyses regarding product reliability  Researched and prepared a technical report on a high volume head with all associated arrays for a robotic liquid handler  Developed methods using UV-VIS detection and Fluorescence to determine a robotic liquid handler's ability to perform serial dilutions  Researched methods to effectively automate PCR of Affymetrix's Genome-Wide Human SNP Anika Therapeutics Research Associate Research and Development Woburn,MA, April 2004 – October 2006  Performed purifications through Flash column chromatography  Analyzed purity and concentration using HPLC (gradient and isocratic methods)  Performed Design-of Experiments (DOE) on both the synthesis and purification of a novel cross linker  Experienced in using rheology to determine viscosity and enzymatic degradation of viscoelastic gels  Performed TLC to determine proper specs for UV absorbance and detection criteria.  Developed proper mobile phase components for chromatographic purification  Developed Batch Records and Manufacturing Directions for multiple development products and prepared technical reports based on original research and data  Initiated and performed screening studies for the use of antioxidants in a formulation  Developed and performed accelerated stability studies to determine the longevity of a product Avecia Biotechnology QC Technician (Contract) Quality Control Milford, MA, January 2004 – February 2004  Responsible for gathering data on TOC and Conductivity for the qualification of a purified water system according to cGMP and USP guidelines, and organizing data for qualification and validation AstraZeneca Research Assistant Cancer Bioscience R&D Waltham, MA, March 2002 - November 2002  Performed DNA Sequencing by Capillary Electrophoresis  Analyzed and reported sequencing data (contig alignments and description of any mutations)
- 3. Raymond P Damien rpdamien@gmail.com  Performed transforming of chemically competent cells for TOPO insertion cloning  Performed PCR to amplify and ligate DNA and its specific primer  Extracted DNA from cultures via spin column methods (Qiagen Mini Prep and Maxi Prep)  Performed sub-cloning for site-directed mutagenesis reactions Millennium Pharmaceuticals Research Assistant Sequencing QA/QC Cambridge, MA, June 1999 – February 2000  Performed DNA extraction via vacuum manifold methods  Performed DNA Sequencing by gel and capillary electrophoresis  Worked with robotic liquid handling devices (Tecan Genesis and a custom build from Cyberlab)  Performed Single Stranded DNA reactions using the M13 bacteriophage, and Double Stranded Reactions on plasmid DNA  Designed Excel Macros for detailing raw data,and developd SOP’s for Documentation Control EDUCATION American International College Springfield, MA  Bachelors of Science degree in Biology, and Chemistry  Awarded May 1999; Honors: GPA 3.3, Dean’s list, United Paper Worker’s Scholarship COMPUTER SKILLS UNIX Sequencher Microsoft Office Suite Photoshop DNA Star Maestro Mantis Bug Tracker Merant Tracker MS SQL iLink Pro PDM Works Quick Test Professional Visio Oracle Agilent Chemstation Minitab Waters Empower Shimadzu OTHER  Vice President of Operations for the Central Mass Cycling Club  Fluent in French/Canadian French