SAAI SURE .pptx
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This document describes Enterococcus faecalis, a common cause of infections in humans. E. faecalis is a gram-positive bacterium found in the gastrointestinal tracts of humans and animals. It is responsible for 80% of enterococcal infections and can cause urinary tract infections, endocarditis, bacteremia, and wound infections. E. faecalis is frequently resistant to multiple antibiotics, making infections difficult to treat. The document introduces an E. faecalis detection kit that can identify pathogenic strains and virulence genes directly from samples like water or food within 6 hours.
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Molecular Biology - Seminary
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The document discusses Enterococcus faecium, a gram-positive bacterium that can be used as an animal probiotic but has also been identified as a cause of hospital-acquired infections. It evaluates 14 potential probiotic E. faecium strains based on guidance from EFSA to demonstrate their safety. Specifically, it examines the strains for a lack of resistance to ampicillin and possession of genetic elements like esp, hylEfm, and IS16 that are associated with hospital-acquired E. faecium. Through laboratory techniques such as PCR and PFGE, the researchers aim to precisely characterize the strains to better inform treatment of infectious outbreaks and public health.Enterococci ppt mahadi
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The document discusses Enterococci, including its natural habitats, morphology, general properties, medically important species, culture characteristics, virulence factors, pathogenicity, laboratory diagnosis, biochemical tests, Lancefield grouping, antibiotic resistance, and treatment options. Key points include that Enterococci normally inhabit the intestinal tract, oral cavity, and vaginal canal of humans and animals. The most common medically important species that cause nosocomial infections are Enterococcus faecalis and E. faecium. Laboratory identification involves culture, biochemical tests like catalase and sugar fermentation reactions, and determining Lancefield grouping. Antibiotic resistance is a concern, especially for vancomycin and penicillin.Enterococcus faecalis: Its Role in Root Canal Treatment Failure and Current C...
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Background- Enterocins are antimicrobial peptides produced by Enterococcus species, which have inhibitory activity on closely
related genera. Streptococcus mutans has been implicated as a pivotal organism to initiate dental caries ensuing, serious
impairment to structure and functions of tooth beside the mental health of humans. Hence we investigated the possibility of using
enterocins in caries treatment and prophylaxis.
Methods- S. mutans was isolated from the saliva of 50 caries-prone humans. Enterococcus faecalis were isolated from the stool
samples of humans. The species identity of the isolated organisms was confirmed using conventional biochemical methods. The
inhibitory activity of enterocins on S. mutans isolates and their active concentrations was identified by spot-on-lawn assay.
Inhibitory activity of 3 enterocins on their target S. mutans isolates were further analyzed by time-kill assay and colony forming
units (CFU)/ml over 0, 4, 8 and 12 h time interval was determined.
Results- Enterocins produced by three E. faecalis isolates demonstrated inhibitory activity on more than 75% of S. mutans isolates.
Enterocins SF101, enterocin SF118 and enterocin PF98 showed 100% inhibition of their target S. mutans at 1:2, 1:4, and undiluted
concentrations. The viable count of enterocin treated S. mutans isolates declined to mean log10 CFU/ml of 4.92 after 12 h time
interval while the untreated control showed the increase to 9.11.
Conclusion- Enterocins exerts bactericidal activity against S. mutans, thus validating the possibility of enterocin to be used for
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- E. coli is also a common cause of urinary tract infections. Certain strains like K1 can cause neonatal meningitis.
- The document outlines techniques for identifying E. coli, including culture media, biochemical tests like IMViC and TSI reactions, and antibiotic treatment options depending on the site of infection.Enterobacteriacae
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This document provides information on Enterobacteriaceae and pathogenic E. coli:
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- Various pathogenic E. coli are described that can cause diseases like urinary tract infections, neonatal meningitis, and intestinal illnesses. Specific strains like ETEC, EIEC, EHEC, EPEC, and EAggEC are mentioned.
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ihsanalsaimary@gmail.com
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The document discusses the Enterobacteriaceae family of bacteria, which includes many pathogens. It focuses on the genus Enterobacter, describing it as a rod-shaped, facultatively anaerobic, Gram-negative bacterium that can cause opportunistic infections. Common media used to culture Enterobacter include Nutrient Agar, EMB agar, and MacConkey agar. Biochemical tests like the indole, citrate, and TSI tests can help identify Enterobacter species.E.coli Dr. Mahadi 
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This document discusses Enterobacteriaceae, a family of Gram-negative bacteria that includes many common pathogens. It provides details on their characteristics, identification, classification based on lactose fermentation, and important genera such as Escherichia coli. Reasons why E. coli is commonly used for gene cloning are described, including its genetic simplicity, rapid growth, safety, extensive prior study, and ability to host foreign DNA. Identification of Enterobacteriaceae involves examining biochemical reactions and growth on selective media like MacConkey agar.Enterobacteriaceae
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Enterobacteria are Gram-negative bacteria in the family Enterobacteriaceae, including pathogenic E. coli. E. coli can cause urinary tract infections, neonatal meningitis, and intestinal diseases. There are five classes of pathogenic E. coli that cause diarrhea - enterotoxigenic E. coli, enteroinvasive E. coli, enterohemorrhagic E. coli, enteropathogenic E. coli, and enteroaggregative E. coli - each with distinct pathogenic mechanisms. E. coli is diagnosed through culture, biochemical tests, and examining reactions on media like MacConkey agar and triple sugar iron agar. Treatment depends on the site and strain of infection.Enterococcus faecalis
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Enterococcus faecalis is a gram-negative bacterium that causes nosocomial infections as well as infections of the GI tract and root canals. It ferments glucose but does not produce catalase, and can cause diseases such as bacteremia, endocarditis, periodontitis, meningitis, UTIs, and wound infections. Enterococcus faecalis is resistant to some antibiotics but sensitive to others, so combined drug therapy is often used to treat infections.Enterobacteriaceae
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Most medically important family of nonspore-forming gram-negative rods.
Most species are normal flora of the GI tract. Salmonella, Shigella, and Yersinia are not normal GI flora.
Major cause of nosocomial infections
Diseases include UTIs, gastroenteritis, septicemia, food poisoning, wound infections, peritonitis, pneumonia, and meningitis
The family exhibits four serological characteristics:
O (somatic) antigen-A cell wall antigen-LPS (heat stable), Used for serological grouping of Salmonella & Shigella.
K (envelope) antigen-Capsular antigen (heat labile)
H (flagellar) antigen-Flagellar antigen-protein (heat labile), Used to serotype Salmonella.
Vi antigen-Capsular antigen of Salmonella Typhi-polysaccharide (heat labile), Role in preventing phagocytosis, may mask O Ag, removed by heating.
Enterobacteriaceae are facultative anaerobes, ferment glucose. Positive nitrate and catalase, non-hemolytic. Except for Plesiomonas, they are oxidase negative.
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Most medically important family of nonspore-forming gram-negative rods.
Most species are normal flora of the GI tract. Salmonella, Shigella, and Yersinia are not normal GI flora.
Major cause of nosocomial infections
Diseases include UTIs, gastroenteritis, septicemia, food poisoning, wound infections, peritonitis, pneumonia, and meningitis
The family exhibits four serological characteristics:
O (somatic) antigen-A cell wall antigen-LPS (heat stable), Used for serological grouping of Salmonella & Shigella.
K (envelope) antigen-Capsular antigen (heat labile)
H (flagellar) antigen-Flagellar antigen-protein (heat labile), Used to serotype Salmonella.
Vi antigen-Capsular antigen of Salmonella Typhi-polysaccharide (heat labile), Role in preventing phagocytosis, may mask O Ag, removed by heating.
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油
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SAAI SURE .pptx
- 2. Enterococcus faecalis E. faecalis is an aerobic, non motile, gram positive coccus (0.5-1 袖m in diameter). Cells are arranged in pairs or as short chains. Responsible for approximately 80% of cases of human infection. Cause many serious human infections, including Urinary Tract Infections, Endocarditis, Bacteremia, and wound infections
- 3. Enterococcus faecalis Commensal , commonly present in the gastrointestinal tracts of humans and other mammals. Urinary Tract Infections are the most common, responsible for approximately 110,000 cases yearly, many of which are nosocomial. Infections with E. faecalis can be especially troublesome to treat because of their frequent resistance to multiple antibiotics.
- 4. Sources of E. faecalis E. faecalis are common in environments, contaminated by human and animal faecal materials. Everyday objects such as telephones, doorknobs, towels, and soaps can also contain E. faecalis. Urban sewage, recipient water, and soil receiving fertilizers of animal origin as well as in food products derived from animals.
- 5. Ecology of E. faecalis Capable of survival at 60 degree celsius for 30 minutes. Able to grow in high pH, salts and acidic condition. Having large amount of natural antibiotic resistant. Alter the nature of the water leads to various kinds of infections.
- 6. Impacts E. faecalis contaminated water is not fit for Human consumption. Cannot be used for healthcare and food processing Industries.
- 7. SAAI Sure E. faecalis Multiplex PCR detection Kit E. faecalis Multiplex PCR detection Kit. Rapid detection for pathogenic E. faecalis and its virulent genes in single step from water, food and other samples
- 8. Description NIOT- E. faecalis kit is an all inclusive, ready to use kit which simplifies and expedite the process of detection of virulent genes in pathogenic E. faecalis (gelE-213 bp, asa1-713 bp, hyl-276 bp ) within 6 hours. 713 bp 276 bp 213 bp
- 9. SAAI Sure - E. faecalis kit contains SAAI Sure - E. faecalis enrichment medium To attain sufficient cell growth within 3 hours Digestion Buffer To extract the crude DNA from the pure and enriched cultures SAAI Sure - E. faecalis Multiplex PCR master mix For specified amplification and detection of virulent genes. Positive and Negative controls To identify and interpret the virulence range of the pathogen
- 10. Applications Agriculture Industries Food Processing Industries Water Industries Healthcare Industries
- 11. Advantages of SAAI Sure First kit to identify the E. faecalis contamination from water, food and other samples. One step detection. Reduced operation handling Increased sensitivity and specificity. Achieve results within 6 hours. Eliminate unintended contamination. Simplified standard operating procedure. Also detect 1-5 CFU/mL
- 12. Prospect Cost Summary Production cost = 8000/Kit Market Price = 15000/Kit Total No. companies in India = 12000 Total turnover of kit = 12000*15000 = 18 crores Total Profit = 8.4 crores (Approximate projection exclusive of all taxes)
- 13. Investment Facility Machinery Manpower Raw materials Regulatory & Statutory Compliance Marketing