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This document summarizes the characterization of a bacteriophage isolated from a soil sample against Mycobacterium smegmatis. Plaques formed by the phage on bacterial lawns exhibited bull's eye morphology. Electron microscopy revealed the phage belongs to the Siphoviridae family with an icosahedral capsid and tail fibers. Gel electrophoresis of restriction enzyme digests estimated the phage genome size to be approximately 70,000 bp. Future experiments will determine the phage's infectivity through a kill curve analysis.
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This study used scanning electron microscopy to examine the protoscoleces and hooks of Echinococcus granulosus collected from infected organs in Libya. SEM images showed morphological variations in the protoscoleces and differences between large and small hooks. Measurements of the hooks found significant statistical differences between hook sizes from different organs and hosts. While some hooks appeared normal, others showed abnormalities in shape, notches or guard structure. This suggests a need to further study relationships between hook morphology and E. granulosus strains to help with identification and epidemiological investigations.Chlorella Vulgaris Alleviates Lead-induced Testicular Toxicity Better than Zi...
Chlorella Vulgaris Alleviates Lead-induced Testicular Toxicity Better than Zi...Prof. Dr. Hesham N. Mustafa 🩺
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SEA Poster!!!
- 1. Introduction Wild-type Methods •Plaques on Plate are bull’s eye, 2-3mm after 24hrs •Electron microscopy- pure population •Size of Genome: about 70,000 bp •Gel electrophoresis: cluster assignment H •Future Directions: Kill curve determination Results Discussion Adsorption of the phagedegradative enzymes or contraction of the sheath & tail @ cell wall  Enzymes degrade bacterial DNA,  genetic recombination  lytic or a lysogenic cycle Michaelangelo temperate phage model: Mycobacterium smegmatis (M. smeg) We hypothesize that a bacteriophage can be isolated and purified from a soil sample; that it can be characterized into a cluster; and that infectivity can be determined in a kill curve soil sample enrichment Purification: streak test, and titer assay. Acknowledgments: Sea faculty Sea Teaching Assistants, Lee Graham, The Hatfull laboratory At Pittsburgh, HHMI, Department of Biological Sciences Figure 1) Agar plate with plaques in M.smeg bacterial lawn. Shown is a Bull’s eye morphology. Note the clear sterile center and the turbid outer rings Figure 2) a typical titer assay with plaques in the M.smeg bacterial lawn. Note the density of the plaques- web pattern Figure 4) Michaelangelo bacteriophage electron microscopy image. At the tip of the tail there is a clover shaped attachment site. Figure 3) Michaelangelo electron microscopy. Note the curved tail characteristic of siphoviridae, icosahedral capsid and 2.5:1 Head:tail ratio Figure 5) Restriction digest. Bands left to right: 1-kb ladder, undigested DNA, BamH1, Cla1, EcoR1, HaeIII, HindIII. Bam H1 did not cut, while HaeII, EcoR1 and HindIII cut. In particular, HaeIII cut into such small fragments it ran off the gel. Cla1 had the clearest bands Stock solution: Medium and high titer lysates DNA extraction: alcohol precipitation Restriction digest and gel eletrophoresis M. Smeg colony formation % survival and Kill curve claI Ìý Ìý Ìý DistanceÌý traveledÌý (cm) logÌý fragmentÌý length FragmentÌý lengthÌý(bd) estimateÌýofÌý genomeÌýsizeÌý (bp) 1.5 4.05 11220 79452 1.6 4.01 10232Ìý 1.6 4.01 10232Ìý 1.8 3.9 7943Ìý 2.2 3.8 6309Ìý 2.5 3.7 5011Ìý 2.7 3.7 5011Ìý 2.9 3.6 3981Ìý 3 3.6 3981Ìý 3.3 3.5 3162Ìý 3.4 3.4 2511Ìý 3.5 3.4 2511Ìý 3.6 3.4 2511Ìý 3.7 3.3 1995Ìý 4 3.2 1584Ìý 4.5 3.1 1258Ìý Bull’s eye morphology Web pattern Gel electrophoresis Siphoviridae Attachment site