This document outlines the presentation for a thesis committee on a study of okra landraces. It includes an introduction providing an overview and statement of the problem being studied. The objectives, hypotheses and significance of the study are then stated. The materials and methods section describes the study site, experimental design, data collection and analysis. Results are then presented and discussed, including correlations between traits. The conclusions identify the most diverse accessions and associations between traits. Recommendations include utilizing certain accessions for breeding and further characterization, genetic mapping of traits, sequencing the okra genome and additional analysis of secondary metabolites and nutritional constituents.
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Senior Thesis Presentation Format
1. COLLEGE OF AGRICULTURE &
SUSTAINABLE DEVELOPMENT
DEPARTMENT OF PSS, NRM OR ASH
17/10/2016 CASD Thesis Committee 1
BY
? Candidate Name (ID´´´.)
? Advisor (s)
´´´´´´´´´´
´´´´´´´´´´
? TITLE OF STUDY
2. OUTLINE OF PRESENTATION
? INTRODUCTION
?Problem Statement
?Hypothesis
?Objectives of Study
?Significance of Study
? MATERIALS AND METHODS
? RESULTS AND DISCUSSION
? CONCLUSIONS AND RECOMMENDATIONS
17/10/2016 CASD THESIS COMMITTEE 2
10. CONCLUSION 1/2
There was intense variability in agro-morphological characters studied among all 29 accessions of okra.
The most diverse and distantly related accessions with outstanding traits were Mamolega, Wune mana
and Cs-Legon and could be potential sources of variability for future breeding work.
Moderate to strong association exists between pairs of quantitative yield traits in the okra landraces
studied.
The output of the PCA revealed that different characters contributed differently to the total variation.
The ISSR primers yielded low level of polymorphism among the 29 accessions; 25 accessions however,
were identified as possible duplicates
Indiana, Yeji-Local, Asante type II and Atomic were detected as distinct unique genotypes and could be
unique sources of genes for improvement programmes.
17/10/2016 CASD THESIS COMMITTEE 10
11. RECOMMENDATIONS
Topmost accessions with desirable qualities (Indiana, Yeji-Local, Asante type II and Atomic) could be utilised for developing
commercial variety for the export market.
Collections should be extended to other regions and base collections in the genebank of PGRRI at Bunso, should be
thoroughly characterised and for further conservation
Genes linked to agronomically important traits in okra should be genetically mapped through Quantitative Traits Loci (QTLs)
Okra genome should be sequenced to develop SSRs
screening for secondary metabolites (HPLC, TLC, LCMS ) should be done
Protein, antioxidants, TFC, TPC of leaves, crude fibre, total carbohydrate, other nutritional constituents (elemental, vitamins
etc) in both pods and leaves should be studied.
17/10/2016 CASD THESIS COMMITTEE 11