際際滷

際際滷Share a Scribd company logo

Small interfering RNA's based detection and diagnosis of plant pathogens

Download as PPTX, PDF
H
HARISH J

RNA interference (RNAi) is a cellular mechanism that recognizes and destroys double stranded RNA. It uses small interfering RNAs (siRNAs) to specifically destroy single stranded and double stranded RNA homologous to the inducer. The host enzymes cut viral RNA into pieces as an antiviral defense mechanism without requiring amplification of the viral nucleic acid. RNAi recognizes dsRNA and eliminates homologous RNAs by cleavage using the enzyme dicer to produce siRNAs, which then bind to ribonuclease proteins to detect and cleave homologous ssRNA, amplifying the process.

1 of 1
Download to read offline
RANI LAKSHMI BAI CENTRAL AGRICULTURAL UNIVERSITY, JHANSI, UTTAR PRADESH-284003 DEPARTMENT OF PLANT PATHOLOGY Submitted to: Dr. Shubha Trivedi Dr. Meenakshi Arya By: Harish J Ag/Pg/0020/19 It is a cytoplasmic surveillance system to recognize double stranded RNA. It specifically destroy ssRNA & dsRNA homologous to the inducer, using small interfering RNAs (siRNA) as a guide. It constitutes antiviral defense mechanism in which host enzymes cut viral RNA into pieces. It does not require any specific amplification or enrichment of Viral nucleic acid. It recognizes dsRNA and eliminates RNAs homologous to the inducer RNA by cleavage using RNase III endonucleases called dicers. siRNAs bind to ribonuclease Hlike proteins in the RNA induced silencing complex(RISC). Used to detect homologous ssRNA molecules for cleavage, producing more siRNAs. RNAi becomes amplified when the cleaved RNA recruits an RNA-directed RNA polymerase to generate more dsRNA. It is again cleaved by a dicer protein to produce secondary si RNAs. Small interfering RNAs(siRNA) based detection and diagnosis of Plant Pathogens

More Related Content

Small interfering RNA's based detection and diagnosis of plant pathogens

  • 1. RANI LAKSHMI BAI CENTRAL AGRICULTURAL UNIVERSITY, JHANSI, UTTAR PRADESH-284003 DEPARTMENT OF PLANT PATHOLOGY Submitted to: Dr. Shubha Trivedi Dr. Meenakshi Arya By: Harish J Ag/Pg/0020/19 It is a cytoplasmic surveillance system to recognize double stranded RNA. It specifically destroy ssRNA & dsRNA homologous to the inducer, using small interfering RNAs (siRNA) as a guide. It constitutes antiviral defense mechanism in which host enzymes cut viral RNA into pieces. It does not require any specific amplification or enrichment of Viral nucleic acid. It recognizes dsRNA and eliminates RNAs homologous to the inducer RNA by cleavage using RNase III endonucleases called dicers. siRNAs bind to ribonuclease Hlike proteins in the RNA induced silencing complex(RISC). Used to detect homologous ssRNA molecules for cleavage, producing more siRNAs. RNAi becomes amplified when the cleaved RNA recruits an RNA-directed RNA polymerase to generate more dsRNA. It is again cleaved by a dicer protein to produce secondary si RNAs. Small interfering RNAs(siRNA) based detection and diagnosis of Plant Pathogens