Snblotting marianela
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This document discusses Southern and Northern blotting techniques. It describes the principles of Southern blotting where DNA is separated by gel electrophoresis, transferred to a membrane, and probed with a labeled DNA probe to detect complementary DNA sequences. It also mentions different fluorogenic substrates that can be used like ECF and DDAO for detecting fluorescence of labeled DNA probes bound to membranes in Southern or Northern blots. Images can then be captured and fluorescence quantified for analysis.
Snblotting marianela
- 1. 5.4 SOUTHERN AND NORTHERN BLOTTING PROGRAMA DE INMUNOLOGÍA MOLECULAR MICROBIANA DRA. YOLANDA LÓPEZ VIDAL DRA. MARIANELA PAREDES MENDOZA 2012
- 2. SOUTHERN Y NORTHERN BLOTTING Figura 1. Principios de Southern blotting
- 3. SOUTHERN Y NORTHERN BLOTTING Figura 1. Principios de Southern blotting
- 4. SUSTRATOS FLUOROGENICOS PARA SOUTHERN Y NORTHER BLOTS Sustrato Exitación max (nm) Emisión max (nm) Aplicación ECF 440 560 Sustrato para la Fosfatasa alcalina DDAO fosfatato 646 660 Sustrato para la Fosfatasa alcalina
- 5. Re p r e s e n t a c i ó n e s q u e m a t i c a p a r a l a d e t e c c i ó n d e l a f l u o r e s c e í n a u n i d a a l a D N A s o n d a e n u n S o u t h e r b l o t
- 6. COMPATIBILIDAD DE LOS SISTEMAS USANDO DIFERENTES SUSTRATOS DDAO, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)
- 7. S O U T H E R N B L O T D E L A D I G E S T I Ó N D E L G E N D E AC T I N A P O R E c o R 1 D E D N A G E N O M I C O H U M A N O. 10.4 µg 0.32 µ CAPTACIÓN DE LA IMAGEN Hibridación Southern ó Northern blots Sustrato (DDAO ó EFC) Imagen
- 8. C UA N T I F I C AC I Ó N D E D N A BA S A D O E N L A M E D I C I Ó N D E L A F L U O R E S C E N C I A D E L O S S O U T H E R M B L O T S