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Рестрикцийн
ферментүүдийн ангилал
  /Types of Restriction
     Endonucleases/
Type I




                           Type II


Рестрикцийн   Ангилал
ферментүүд    /хэлбэр/



                           Type III




                         Artificial RE
рестрикцийн ферментүүдийн ангилал
рестрикцийн ферментүүдийн ангилал
ДНХ палиндром

                           палиндром нь ДНХ-ийн дан
                     гинжин дэх урд болон ардаасаа
                     ижил уншигддаг -GTAATG

                                           палиндром
                     нь мөн л урд ардаасаа уншигдах ,
                     нуклеотидийн дараалал боловч энэ
                     дараалал нь комплементар ДНХ-
                     ийн утсан дээр байрладагаараа
                     дээрхээс ялгаатай (GTATAC
                     CATATG-тэй комплементар)
                                          палиндром
                     нь        палиндром-с харьцангуй
                     түгээмэл байхын зэрэгцээ биологид
                     маш чухал үүрэгтэй.


Рестрикцийн сайт?
рестрикцийн ферментүүдийн ангилал
рестрикцийн ферментүүдийн ангилал
- Шулуун төгсгөл гэдэг РФ-ийн нөлөөгөөр
  зүсэгдсэн ДНХ молекулын илүү гарсан суурь
  агуулаагүй хэсэг
- Наалданги төгсгөл гэдэг нь РФ-ийн зүсэлтийн
   үр дүнд үүссэн ДНХ-ийн илүү суурьтай
   богино дан гинжний хэсэг юм.
HindIII




 EcoRI




   AluI




 HaeIII
рестрикцийн ферментүүдийн ангилал
Example: EcoR1
 Genus: Escherichia
 Species: coli
 Strain: R
 Order discovered: 1
рестрикцийн ферментүүдийн ангилал
рестрикцийн ферментүүдийн ангилал
рестрикцийн ферментүүдийн ангилал
Мөн 2 дугаар хэлбэр дараах
 бүлгүүдэд хуваагдана.
Type IIB (BcgI, BplI)
Type IIE (NaeI)
Type IIF (NgoMIV)
Type IIG (Eco57I)
Type IIM (DpnI)
Type IIS (FokI)
Type IIT (Bpu10I, BslI)
 EC 3.1.21.5
 Large enzymes
 Combination restriction-and-modification
 Cleave outside of their recognition
  sequences /20-30 base pairs after the
  recognition site/
 Require two recognition sequences in
  opposite orientations within the same
  DNA molecule
 Cleave only normal and modified DNA
  (methylated, hydroxymethylated and
  glucosyl-hydroxymethylated bases).
 Recognition sequences have not been well
  defined
 Cleavage takes place ~30 bp away from one
  of the sites
 generated by fusing a natural or engineered DNA
  binding domain to a nuclease domain
 can target large DNA sites (up to 36 bp)
 can be engineered to bind to desired DNA
  sequences
Ашигласан эх
                            сурвалжууд
1. Roche Applied Science Restriction Enzymes FAQS
   and Ordering Guide
2. Стент Г. Молекулярная биология вирусов
   бактерий. Пер. с англ.
   1965. Твердый переплет. 468 с.
   Предварительный заказ.
3. http://www.slideshare.net/ezzymo9/newsfeed
4. http://en.wikipedia.org/wiki/Restriction_enzyme
5. http://www.dnai.org/b/index.html
рестрикцийн ферментүүдийн ангилал

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рестрикцийн ферментүүдийн ангилал

  • 2. Type I Type II Рестрикцийн Ангилал ферментүүд /хэлбэр/ Type III Artificial RE
  • 5. ДНХ палиндром палиндром нь ДНХ-ийн дан гинжин дэх урд болон ардаасаа ижил уншигддаг -GTAATG палиндром нь мөн л урд ардаасаа уншигдах , нуклеотидийн дараалал боловч энэ дараалал нь комплементар ДНХ- ийн утсан дээр байрладагаараа дээрхээс ялгаатай (GTATAC CATATG-тэй комплементар) палиндром нь палиндром-с харьцангуй түгээмэл байхын зэрэгцээ биологид маш чухал үүрэгтэй. Рестрикцийн сайт?
  • 8. - Шулуун төгсгөл гэдэг РФ-ийн нөлөөгөөр зүсэгдсэн ДНХ молекулын илүү гарсан суурь агуулаагүй хэсэг - Наалданги төгсгөл гэдэг нь РФ-ийн зүсэлтийн үр дүнд үүссэн ДНХ-ийн илүү суурьтай богино дан гинжний хэсэг юм.
  • 9. HindIII EcoRI AluI HaeIII
  • 11. Example: EcoR1 Genus: Escherichia Species: coli Strain: R Order discovered: 1
  • 15. Мөн 2 дугаар хэлбэр дараах бүлгүүдэд хуваагдана. Type IIB (BcgI, BplI) Type IIE (NaeI) Type IIF (NgoMIV) Type IIG (Eco57I) Type IIM (DpnI) Type IIS (FokI) Type IIT (Bpu10I, BslI)
  • 16.  EC 3.1.21.5  Large enzymes  Combination restriction-and-modification  Cleave outside of their recognition sequences /20-30 base pairs after the recognition site/  Require two recognition sequences in opposite orientations within the same DNA molecule
  • 17.  Cleave only normal and modified DNA (methylated, hydroxymethylated and glucosyl-hydroxymethylated bases).  Recognition sequences have not been well defined  Cleavage takes place ~30 bp away from one of the sites
  • 18.  generated by fusing a natural or engineered DNA binding domain to a nuclease domain  can target large DNA sites (up to 36 bp)  can be engineered to bind to desired DNA sequences
  • 19. Ашигласан эх сурвалжууд 1. Roche Applied Science Restriction Enzymes FAQS and Ordering Guide 2. Стент Г. Молекулярная биология вирусов бактерий. Пер. с англ. 1965. Твердый переплет. 468 с. Предварительный заказ. 3. http://www.slideshare.net/ezzymo9/newsfeed 4. http://en.wikipedia.org/wiki/Restriction_enzyme 5. http://www.dnai.org/b/index.html