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The Dos and Don'ts of Environmental Sampling

ABC Research Laboratories
ABC Research Laboratories

The document provides instructions for proper environmental sampling. It outlines the supplies that will be provided in a sampling kit from ABC Research Laboratories, including sample bags, submission forms, and freeze packs. It details the proper procedures for filling out forms, wearing gloves, labeling samples, swabbing or wiping target areas, securing samples, and shipping in a cooler to maintain refrigeration. Following these steps ensures reliable and defensible environmental monitoring data.

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The Dos and Donts of Environmental Sampling By Ivy Cho 1.866.233.5884 ivyc@abcr.com
What is Environmental Sampling? ≒ It is a risk assessment used to monitor the quality of the environment ≒ The use of proper sampling methods is crucial for ensuring reliable and defensible data.
What do I do? ≒ ABC Research Laboratories will provide you with a kit for environmental sampling, which includes the following supplies: Sponges or swabs Freezie packs Sample submission form Written instructions for sampling
The Dos ≒ Fill out the sample submission form correctly with the analyses requested and clear descriptions of the samples Note: The way the sample submission form is completed will reflect your Certificate of Analysis ≒ Place your sample submission form in a re-sealable bag to prevent it form getting wet
The Donts ≒ Do not use the same sterile gloves for all environmental sampling New sterile gloves must be used between samples ≒ Do not ship your samples ambient, warmer conditions will allow for higher microbial counts Place enough freeze packs to keep the samples in refrigerated conditions
Steps to Proper Sampling YouTube Video: How to Conduct Environmental Sampling click here Environmental Sampling Video
STEP 1 - Labeling ≒ Label all sample bags with a sample number of the sampling site with a permanent marker ≒ The sample number on the sample bag should match the sampling site description on the sample submission form E.g. Sample #1 = large drain in facility room 4
Step 2 - Hygiene ≒ What does the sampler need to do before wiping the sampling site? Wear clean laboratory wear (recommended) Must wash hands to mid-arms with warm water and antibacterial soap to mid forearm (see image)
Step 3 & 4 ≒ How to open the sample bag to swab Keep the sample bag closed Push the sponge up towards the opening ≒ Be sure not to open the bag or touch the sponge with your fingers to prevent contamination. ≒ Use the red tabs at the top of the sample bag to open (this will help keep the bag open) ≒ Place the opened bag on a clean surface
Step 5 ≒ With sterile gloves on (provided with the sampling bag), remove the sponge from the bag ≒ Avoid touching the surface of the sponge with anything other than the sterile glove and the sampling site to be tested
Step 6 For Sponges ≒ Wipe the sponge over the target area described in your environmental monitoring plan E.g. 10by 10 target area, 10 tens vertically (on one side) and 10 times horizontally (on the other side) ≒ Avoid putting strong pressure while swabbing to prevent the sponge from crumbling
Step 7 For Swabs ≒ Wipe the swab over the target area described in your environmental monitoring plan E.g. 2by 2 target area, vertically (on one side) and horizontally (on the other side)
Step 8 ≒ Place the sponge back in the sampling bag after wiping the target area Avoid having the sponge touch the outside of the bag to prevent contamination ≒ Secure the swab in its plastic tube without touching the edges or exterior of the tube
Step 9 ≒ After placing the sample back in the sampling bag, expel excess air from the bag ≒ Take the wire on top of the sampling bag and fold over 3 or 4 times to close ≒ Secure the bag by folding the attached wire in the middle and twist the end wires together For extra security, place the sample bag in a re- sealable plastic bag
Step 10 - Shipping ≒ Ship the sample overnight to ABC Research Laboratories in a sturdy insulated cooler ≒ Samples must arrive in refrigerated conditions
Questions? ≒ For additional information, please contact us at 352.372.0436 or visit our website at abcr.com

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The Dos and Don'ts of Environmental Sampling

  • 1. The Dos and Donts of Environmental Sampling By Ivy Cho 1.866.233.5884 ivyc@abcr.com
  • 2. What is Environmental Sampling? ≒ It is a risk assessment used to monitor the quality of the environment ≒ The use of proper sampling methods is crucial for ensuring reliable and defensible data.
  • 3. What do I do? ≒ ABC Research Laboratories will provide you with a kit for environmental sampling, which includes the following supplies: Sponges or swabs Freezie packs Sample submission form Written instructions for sampling
  • 4. The Dos ≒ Fill out the sample submission form correctly with the analyses requested and clear descriptions of the samples Note: The way the sample submission form is completed will reflect your Certificate of Analysis ≒ Place your sample submission form in a re-sealable bag to prevent it form getting wet
  • 5. The Donts ≒ Do not use the same sterile gloves for all environmental sampling New sterile gloves must be used between samples ≒ Do not ship your samples ambient, warmer conditions will allow for higher microbial counts Place enough freeze packs to keep the samples in refrigerated conditions
  • 6. Steps to Proper Sampling YouTube Video: How to Conduct Environmental Sampling click here Environmental Sampling Video
  • 7. STEP 1 - Labeling ≒ Label all sample bags with a sample number of the sampling site with a permanent marker ≒ The sample number on the sample bag should match the sampling site description on the sample submission form E.g. Sample #1 = large drain in facility room 4
  • 8. Step 2 - Hygiene ≒ What does the sampler need to do before wiping the sampling site? Wear clean laboratory wear (recommended) Must wash hands to mid-arms with warm water and antibacterial soap to mid forearm (see image)
  • 9. Step 3 & 4 ≒ How to open the sample bag to swab Keep the sample bag closed Push the sponge up towards the opening ≒ Be sure not to open the bag or touch the sponge with your fingers to prevent contamination. ≒ Use the red tabs at the top of the sample bag to open (this will help keep the bag open) ≒ Place the opened bag on a clean surface
  • 10. Step 5 ≒ With sterile gloves on (provided with the sampling bag), remove the sponge from the bag ≒ Avoid touching the surface of the sponge with anything other than the sterile glove and the sampling site to be tested
  • 11. Step 6 For Sponges ≒ Wipe the sponge over the target area described in your environmental monitoring plan E.g. 10by 10 target area, 10 tens vertically (on one side) and 10 times horizontally (on the other side) ≒ Avoid putting strong pressure while swabbing to prevent the sponge from crumbling
  • 12. Step 7 For Swabs ≒ Wipe the swab over the target area described in your environmental monitoring plan E.g. 2by 2 target area, vertically (on one side) and horizontally (on the other side)
  • 13. Step 8 ≒ Place the sponge back in the sampling bag after wiping the target area Avoid having the sponge touch the outside of the bag to prevent contamination ≒ Secure the swab in its plastic tube without touching the edges or exterior of the tube
  • 14. Step 9 ≒ After placing the sample back in the sampling bag, expel excess air from the bag ≒ Take the wire on top of the sampling bag and fold over 3 or 4 times to close ≒ Secure the bag by folding the attached wire in the middle and twist the end wires together For extra security, place the sample bag in a re- sealable plastic bag
  • 15. Step 10 - Shipping ≒ Ship the sample overnight to ABC Research Laboratories in a sturdy insulated cooler ≒ Samples must arrive in refrigerated conditions
  • 16. Questions? ≒ For additional information, please contact us at 352.372.0436 or visit our website at abcr.com