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Can Aspargaus
inhibit population
 growth of yeast
      cells?
           Tiffany Sato
  Experium Science Academy
 Marlborough School, Ninth Grade
Hypothesis and Goals
 During digestion, asparagus produces sulfur
   compounds, which is beneficial in the reduction of
   the growth of cancer cells (Mitchell, 2001).
   Therefore, I decided to investigate this further which
   led me to the information that asparagus produced
   high levels of sulfur. From this, I decided to start
   studying the effect of asparagus on yeast activity.

 I wanted to see a decrease in yeast activity
   compared to a negative and positive control. A
   decrease in growth meant that the asparagus, the
   high amounts of sulfur, was inhibiting the growth of
   the yeast cells.
Why I used Yeast Cells
  and the Similarities
 Yeast Cells                Cancer Cells
   (Saccharomyces              Typical eukaryotic
   cerevisiae)                   structure
    Typical eukaryotic        Fast and
      structure                  incontrollable cell
    Fast and                    division
      incontrollable cell
      division
    Economically
      accessible
    Genetic material is
      known
Yeast Cells as a Model
        Organism
 I realized that scientific studies have shown
   thatSaccharomyces cerevisiae could be used as an
   alternative to cancer cells when doing research.
   (Hartwell, 2001)         (Mortimer, 1973)
Research Methods
Can Asparagus
        inhibit population
      growth in yeast cells?


  Yeast Activity       Viable Colony
   Growth with        Counts with Agar
Spectrophotometer         Plates
Recipes
Positive (YPD+)

 1% Yeast Extract
 2% Peptone
 1% Dextrose

Negative (YPD-)

 Water

Experimental (YPDAsp)

   1% Yeast Extract
   2% Peptone
   1% Dextrose
   50mL of Filtered Asparagus
Yeast Activity Growth
with Spectrophotometer
Grow S. cerevisiae                                         Centrifuge all
                              Incubated all
   in YPD+, YPD-                                           the different
                          cultures for 24 hours
 , YPDAsp Broths                                             mediums




      Measured the
    absorbance of each
          using a                                  Compared the Yeast
   spectrophotometer at                           Activity Growth Results
          600nm
Viable Colony Counts
     using Agar Plates
 For my agar
    plates, I                                          Streak onto my
 inoculated a                                          three different
                          Incubate for 48
                                                            plates:
colony from a              hours at 30oC
                                                      positive, negative
  colony off a                                        and experimental
positive plate.




  Incubate for 24 hours                     Record Viable Colony
        at 30o C                               Count Results
Results for Yeast Growth
         Activity
Absorbance of Yeast
                             Activity Sample
                      1
                     0.9
                     0.8
Absorbance (600nm)




                     0.7
                     0.6
                     0.5                              POSITIVE
                     0.4                              NEGATIVE
                     0.3                              EXPL

                     0.2
                     0.1
                      0
                           0    5           10   15
                                Sample Numbers
Data Table
Data

 Average for Negative (without any nutrients) :
   0.189 abs

 Average for Experimental (with asparagus):
   0.488 abs

 Average for Positive (with nutrients, without
   asparagus):0.633 abs
Conclusions for Yeast
     Growth Activity
 My results from my experiment with the liquid
   medium with asparagus had the lowest absorbance
   (0.488 abs) compared to my positive control
   (0.633 abs ) and higher absorbance compared to
   my negative control (0.189 abs). This indicates a
   decrease in yeast activity upon addition of
   asparagus indicating minimal cell growth.
Results for Viable
   Colonies
Experimental
Results
Comparison of the
       Controls
Negative Experiment   Positive Experiment
Conclusions from Viable
       Colonies
 Although viable colonies have limited
   quantification, the pictures show the apparent
   difference of structural colonies between the
   Positive, Experimental, and Negative.


       Positive         Experimental        Negative

    Raised, Corvex    Flat Surface     Produced no
     Surface                              colonies
Acknowledgements
 Thank you to Sigma Xi for this opportunity.
 Thank you to RaudhahRahman, Experium
Science Academy, my parents, and those who
      have supported me throughout.

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  • 1. Can Aspargaus inhibit population growth of yeast cells? Tiffany Sato Experium Science Academy Marlborough School, Ninth Grade
  • 2. Hypothesis and Goals During digestion, asparagus produces sulfur compounds, which is beneficial in the reduction of the growth of cancer cells (Mitchell, 2001). Therefore, I decided to investigate this further which led me to the information that asparagus produced high levels of sulfur. From this, I decided to start studying the effect of asparagus on yeast activity. I wanted to see a decrease in yeast activity compared to a negative and positive control. A decrease in growth meant that the asparagus, the high amounts of sulfur, was inhibiting the growth of the yeast cells.
  • 3. Why I used Yeast Cells and the Similarities Yeast Cells Cancer Cells (Saccharomyces Typical eukaryotic cerevisiae) structure Typical eukaryotic Fast and structure incontrollable cell Fast and division incontrollable cell division Economically accessible Genetic material is known
  • 4. Yeast Cells as a Model Organism I realized that scientific studies have shown thatSaccharomyces cerevisiae could be used as an alternative to cancer cells when doing research. (Hartwell, 2001) (Mortimer, 1973)
  • 6. Can Asparagus inhibit population growth in yeast cells? Yeast Activity Viable Colony Growth with Counts with Agar Spectrophotometer Plates
  • 7. Recipes Positive (YPD+) 1% Yeast Extract 2% Peptone 1% Dextrose Negative (YPD-) Water Experimental (YPDAsp) 1% Yeast Extract 2% Peptone 1% Dextrose 50mL of Filtered Asparagus
  • 8. Yeast Activity Growth with Spectrophotometer Grow S. cerevisiae Centrifuge all Incubated all in YPD+, YPD- the different cultures for 24 hours , YPDAsp Broths mediums Measured the absorbance of each using a Compared the Yeast spectrophotometer at Activity Growth Results 600nm
  • 9. Viable Colony Counts using Agar Plates For my agar plates, I Streak onto my inoculated a three different Incubate for 48 plates: colony from a hours at 30oC positive, negative colony off a and experimental positive plate. Incubate for 24 hours Record Viable Colony at 30o C Count Results
  • 10. Results for Yeast Growth Activity
  • 11. Absorbance of Yeast Activity Sample 1 0.9 0.8 Absorbance (600nm) 0.7 0.6 0.5 POSITIVE 0.4 NEGATIVE 0.3 EXPL 0.2 0.1 0 0 5 10 15 Sample Numbers
  • 13. Data Average for Negative (without any nutrients) : 0.189 abs Average for Experimental (with asparagus): 0.488 abs Average for Positive (with nutrients, without asparagus):0.633 abs
  • 14. Conclusions for Yeast Growth Activity My results from my experiment with the liquid medium with asparagus had the lowest absorbance (0.488 abs) compared to my positive control (0.633 abs ) and higher absorbance compared to my negative control (0.189 abs). This indicates a decrease in yeast activity upon addition of asparagus indicating minimal cell growth.
  • 15. Results for Viable Colonies
  • 17. Comparison of the Controls Negative Experiment Positive Experiment
  • 18. Conclusions from Viable Colonies Although viable colonies have limited quantification, the pictures show the apparent difference of structural colonies between the Positive, Experimental, and Negative. Positive Experimental Negative Raised, Corvex Flat Surface Produced no Surface colonies
  • 19. Acknowledgements Thank you to Sigma Xi for this opportunity. Thank you to RaudhahRahman, Experium Science Academy, my parents, and those who have supported me throughout.