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''VESICLE TETHERING'' Presentation By KATE, Wisdom Deebeke

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Wisdom Deebeke Kate
Wisdom Deebeke Kate

Following protein biogenesis, proteins are trafficked to different cellular compartments within the cell from either the endoplamsic reticulum, or (mostly) the golgi apparatus. This assessed presentation was delivered to a group of fellow students during the stage 2 (in April 2013) of my studies.

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Presentation By Kate, Wisdom Deebeke on Vesicle Tethering Objective To understand the mechanism of Vesicle Tethering.
Vesicle Tethering, what is it? Formation of physical links between v- and t-SNARES Formed before TRANS SNARE complex formation Aimed at promoting specificity between two membranes Growing number of factors involved now identified, all recruited by Rab GTPase.
Tethering factors Two broad classes of molecules identified: a) Long coiled-coil proteins Form homodimeric coiled-coils E.g. Uso1p (in yeast) and P115 (in mammals) Essential for tethering ER-derived vesicles b) Multisubunit tethering complexes 7 large conserved complexes proposed Initially identified in yeasts E.g. include exocyst, COG complex, TRAPP, etc. TRAPP-1 involved in ER-Golgi anterograde transport Image from: Whyte, J. R. C. and S. Munro (2002). "Vesicle tethering complexes in membrane traffic." Journal of Cell Science 115(13): 2627-2637.
Summary Vesicle tethering important for specific localisation of transport vesicles on membranes Many factors involved have been identified and characterised Rab GTPase plays important role in Tethering factor recruitment References: 1. Whyte, J. R. C. and S. Munro (2002). "Vesicle tethering complexes in membrane traffic." Journal of Cell Science 115(13): 2627-2637 2. Olkkonen, V. M. and E. Ikonen (2006). "When intracellular logistics fails - genetic defects in membrane trafficking." Journal of Cell Science 119(24): 5031-5045.
THANK YOU FOR YOUR ATTENTION ANY QUESTIONS...?

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''VESICLE TETHERING'' Presentation By KATE, Wisdom Deebeke

  • 1. Presentation By Kate, Wisdom Deebeke on Vesicle Tethering Objective To understand the mechanism of Vesicle Tethering.
  • 2. Vesicle Tethering, what is it? Formation of physical links between v- and t-SNARES Formed before TRANS SNARE complex formation Aimed at promoting specificity between two membranes Growing number of factors involved now identified, all recruited by Rab GTPase.
  • 3. Tethering factors Two broad classes of molecules identified: a) Long coiled-coil proteins Form homodimeric coiled-coils E.g. Uso1p (in yeast) and P115 (in mammals) Essential for tethering ER-derived vesicles b) Multisubunit tethering complexes 7 large conserved complexes proposed Initially identified in yeasts E.g. include exocyst, COG complex, TRAPP, etc. TRAPP-1 involved in ER-Golgi anterograde transport Image from: Whyte, J. R. C. and S. Munro (2002). "Vesicle tethering complexes in membrane traffic." Journal of Cell Science 115(13): 2627-2637.
  • 4. Summary Vesicle tethering important for specific localisation of transport vesicles on membranes Many factors involved have been identified and characterised Rab GTPase plays important role in Tethering factor recruitment References: 1. Whyte, J. R. C. and S. Munro (2002). "Vesicle tethering complexes in membrane traffic." Journal of Cell Science 115(13): 2627-2637 2. Olkkonen, V. M. and E. Ikonen (2006). "When intracellular logistics fails - genetic defects in membrane trafficking." Journal of Cell Science 119(24): 5031-5045.
  • 5. THANK YOU FOR YOUR ATTENTION ANY QUESTIONS...?
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