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Work done a Centre for Soft and Condensed

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Adwaith P. Suratkar
Adwaith P. Suratkar

This document summarizes work done at a Centre for Soft and Condensed Matter, including the fabrication and characterization of glass nano-pipettes and micro-pipettes with diameters ranging from 74.36nm to 176.1nm. Conductance measurements were taken for the pipettes using salt buffer solutions of varying molarity. Experiments visualized the oscillation and translocation of polystyrene beads through pipettes when subjected to alternating electric fields and pressure gradients, allowing the measurement of bead velocity and trajectory. Imaging of single bead translocations provide insight into transport phenomena.

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Work done a Centre for Soft and Condensed Matter.
Fabrication of glass nano-pipettes and micro-pipettes
Pore diameter: 104.7 nm Shrunk diameter: 74.36 nm Pore diameter: 88.62 nm Shrunk diameter: 54.78 nm Shrinking of glass nanopores
Device characterization I-V curves for 5 salt buffer concentrations. Using the I-V curves calculate the conductance. Experimental Set up and reading Pore diameter = 176.1 nm Molarity Resistance (MΩ) Irms (Noise) pA Beta value (β) Alpha Value (α) 1M 2.13 8.47 0.1 0.5 0.1M 7.75 3.94 0.1 0.5 50mM 11.34 4.33 0.1 0.5 1mM 87.53 3.91 0.1 0.5 100µM 95.99 3.41 0.1 0.5 1µM 98.15 3.87 0.1 0.5
Results
Work done a Centre for Soft and Condensed
Flow cell designed for imaging pipettes under 20X and 40X objective
Micro fluidic cell designed for pressure and diffusion experiments through pipettes.
Flow cell designed for visualizing electrical and pressure driven translocation events through pipettes.
Oscillation of 1 micron polystyrene beads in alternating electric field. Position A Position B Polystyrene bead Function generator
Calculating the velocity of 1 micron polystyrene beads oscillating in alternating electric field.
1a 1b 1c 1d 5a 5b 5c 5d 5e Translocation of Polystyrene beads through sub micron glass pipettes Fig (1a-1d) translocation of 15 micron beads through 50 micron pipette Fig (5a-5e) translocation of fluorescent 15 micron beads through 30 micron pipette
2a 2b 2c 2d 3a 3b 3c 3d 3e 4a 4b 4c 4d Fig (2a-2d); (3a-3e); (4a-4d) – Translocation of 3 micron beads through 5 micron pipette
Pressure driven Translocation of 450 nm polystyrene beads through a 900 nm pipette, image taken under 40X objective.
Pressure driven diffusion through pipettes to track the trajectory of polystyrene beads at the mouth of the micropipette
Pore diameter: 1.6 µm Translocation and visualization of polystyrene beads (500 nm and 1 µm) under alternating electrical stimulus. Flow cell used for visualizing and performing translocation events.
1 2 3 4 5 6 7 8 Visualizing a single electrical translocation event, of a 1 micron polystyrene bead oscillating under alternating field at 3V.
1 5 2 4 3 6 7

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Work done a Centre for Soft and Condensed

  • 1. Work done a Centre for Soft and Condensed Matter.
  • 2. Fabrication of glass nano-pipettes and micro-pipettes
  • 3. Pore diameter: 104.7 nm Shrunk diameter: 74.36 nm Pore diameter: 88.62 nm Shrunk diameter: 54.78 nm Shrinking of glass nanopores
  • 4. Device characterization I-V curves for 5 salt buffer concentrations. Using the I-V curves calculate the conductance. Experimental Set up and reading Pore diameter = 176.1 nm Molarity Resistance (MΩ) Irms (Noise) pA Beta value (β) Alpha Value (α) 1M 2.13 8.47 0.1 0.5 0.1M 7.75 3.94 0.1 0.5 50mM 11.34 4.33 0.1 0.5 1mM 87.53 3.91 0.1 0.5 100µM 95.99 3.41 0.1 0.5 1µM 98.15 3.87 0.1 0.5
  • 7. Flow cell designed for imaging pipettes under 20X and 40X objective
  • 8. Micro fluidic cell designed for pressure and diffusion experiments through pipettes.
  • 9. Flow cell designed for visualizing electrical and pressure driven translocation events through pipettes.
  • 10. Oscillation of 1 micron polystyrene beads in alternating electric field. Position A Position B Polystyrene bead Function generator
  • 11. Calculating the velocity of 1 micron polystyrene beads oscillating in alternating electric field.
  • 12. 1a 1b 1c 1d 5a 5b 5c 5d 5e Translocation of Polystyrene beads through sub micron glass pipettes Fig (1a-1d) translocation of 15 micron beads through 50 micron pipette Fig (5a-5e) translocation of fluorescent 15 micron beads through 30 micron pipette
  • 13. 2a 2b 2c 2d 3a 3b 3c 3d 3e 4a 4b 4c 4d Fig (2a-2d); (3a-3e); (4a-4d) – Translocation of 3 micron beads through 5 micron pipette
  • 14. Pressure driven Translocation of 450 nm polystyrene beads through a 900 nm pipette, image taken under 40X objective.
  • 15. Pressure driven diffusion through pipettes to track the trajectory of polystyrene beads at the mouth of the micropipette
  • 16. Pore diameter: 1.6 µm Translocation and visualization of polystyrene beads (500 nm and 1 µm) under alternating electrical stimulus. Flow cell used for visualizing and performing translocation events.
  • 17. 1 2 3 4 5 6 7 8 Visualizing a single electrical translocation event, of a 1 micron polystyrene bead oscillating under alternating field at 3V.