This document provides instructions for making yeast culture medium and growing yeast cultures. It includes a list of ingredients for the yeast culture medium, such as sugar, agar agar, meat extract, and yeast extract. It also provides a step-by-step protocol for making and storing the yeast culture medium and petri dishes. Safety rules and required lab equipment are outlined as well, including tying back hair, washing hands, wearing protective gear, and cleaning work spaces. Inspiration sources and image credits are listed at the end.
3. biotechnology
noun bio·tech·nol·o·gy ˌbī-ō-tek-ˈnä-lə-jē
: the use of living cells, bacteria, etc., to make useful products (such as
crops that insects are less likely to destroy or new kinds of medicine)
Merriam-Wbster’s Learners’s Dictionary
7. Yeast culture medium
20g Sugar
15g Agar Agar
requirement for 1l
protocol
Weigh the ingriedents | Put everything, except the Agar Agar, in a Beaker | Add the
water | S@r | Pour the mix into a boDle | Add the Agar Agar | Screw the lid of the
boDle half way | Autoclave for 20’(using a pressure cooker) | Let it cool down not below
50 C°, as it will become gel | Cast the Petri Dishes | Wait for them to dry
10g Meat Extract (e.g. Liebig)
10g Yeast Extract (e.g. Marmite)
12g Food dye
1 l Water
note
The boDle can be stored at room temperature, remember to screw the lid.
The Petri dishes can be sealed and stored in the fridge.