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rnaseq gene prediction disease genes genome nextgen sequencing library preparation genome sequencing fasta blast scoring matrices multiple sequence alignment barcode of life species identification indian biodiversity databases eol gencode human genome encode 2012 bioinformatics workshop gene ontology structural annotation primer design pattern profile motif genome online database plant and animal databases nucleotide and protein databases bar code of life biological databases parametric non-parametric statistics disease genome tools cufflink sequencing chi square test light weight eumicrobedb genetics and genomics gene prediction in eukaryotes restriction mapping primer designing positional cloning