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apoptosis toxicity hca preclinical screening cancer cell line tumor phospholipidosis primary cell model mtor akt her2 erlotinib expression egfr genotype growth inhibition biomarker gene proliferation mrna sanger lapatinib igfr cytotoxicity braf genetic correlation resistant kinase inhibitors biomarkers mek cellular assays in vitro phenotypic drug discovery anti-cancer personalized medicine preclinical sensitivity micronuclei micronucleus genotoxicity genetic toxicity steatosis luminex primary human cells hepatotoxicity mutation